Developmental effects of exposure to ultraviolet B radiation on the freshwater prawn Macrobrachium olfersi: Mitochondria as a target of environmental UVB radiation

Quadros, Thaline de; Schramm, Heloísa; Zeni, Eliane Cristina; Simioni, Carmen; Allodi, Silvana; Müller, Yara Maria Rauh; Ammar, Dib; Nazari, Evelise Maria

doi:10.1016/j.ecoenv.2016.06.018

Cited by 16 publications

(4 citation statements)

References 53 publications

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“…In addition, the master sensor of cellular energetic status, Ampk, was also upregulated by exposure to 0.2 W/m 2 UVB (Figure A), indicating cell-wide energy shortage. The effects of UVB on mitochondrial energetic functions have not been well investigated in crustaceans, albeit a study on the freshwater prawn Macrobrachium olfersi reported dramatic morphological changes in the mitochondria, such as disrupted outer and inner membranes, mitochondrial fission, and reduction in the number of mitochondrial crests after 30 min of exposure to a high level (irradiance: 3100 W/m 2 , total dose: 5.58 kJ/m 2 ) of UVB . ATP depletion is known to also affect ATP-dependent processes such as membrane ion transport , and trigger necrotic cell death, which may contribute to organ dysfunctions and mortality in D.…”

Section: Resultsmentioning

confidence: 99%

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Shang

Xie

Lee

et al. 2020

Environ. Sci. Technol.

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Ultraviolet B (UVB) radiation is a natural nonchemical stressor posing potential hazards to organisms such as planktonic crustaceans. The present study was conducted to revisit the lethal effects of UVB on crustaceans, generate new experimental evidence to fill in knowledge gaps, and develop novel quantitative adverse outcome pathways (qAOPs) for UVB. A combination of laboratory and computational approaches was deployed to achieve the goals. For targeted laboratory tests, Daphnia magna was used as a prototype and exposed to a gradient of artificial UVB. Targeted bioassays were used to quantify the effects of UVB at multiple levels of biological organization. A toxicity pathway network was assembled based on the new experimental evidence and previously published data extracted using a novel computational tool, the NIVA Risk Assessment Database (NIVA RAdb). A network of AOPs was developed, and weight of evidence was assessed based on a combination of the current and existing data. In addition, quantitative key event relationships in the AOPs were developed by fitting the D. magna data to predefined models. A complete workflow for assembly and evaluation of qAOPs has been presented, which may serve as a good example for future de novo qAOP development for chemical and nonchemical stressors.

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Section: Resultsmentioning

confidence: 99%

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Shang

Xie

Lee

et al. 2020

Environ. Sci. Technol.

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“…We show that exposure to environmental radionuclides impacts mitochondrial homeostasis in the brains of bank voles via an increase in (1) mtDNA copy number and (2) mtDNA damage, and (3) can impact the association between PGC-1α expression and mtDNA copy number. Exposure to genotoxic stressors such as radiation, pollutants, oxidative stress or DNA damaging chemicals induce an increase in mitochondrial biogenesis (Lin et al 2013;Correia-Melo et al 2014;Kaur et al 2014;de Quadros et al 2016). In addition, damage to mitochondria can alter mitochondrial function and cellular energy metabolism (Salin et al 2012;Jayasundara 2017).…”

Section: Discussionmentioning

confidence: 99%

Exposure to environmental radionuclides alters mitochondrial DNA maintenance in a wild rodent

et al. 2020

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Mitochondria are sensitive to oxidative stress, including that derived from ionizing radiation. To quantify the effects of exposure to environmental radionuclides on mitochondrial DNA (mtDNA) dynamics in wildlife, bank voles (Myodes glareolus) were collected from the chernobyl exclusion zone (CEZ), where animals are exposed to elevated levels of radionuclides, and from uncontaminated areas within the CEZ and elsewhere in Ukraine. Brains of bank voles from outside the CEZ were characterized by low mtDNA copy number and low mtDNA damage; by contrast, bank voles within the CEZ had high mtDNA copy number and high mtDNA damage, consistent with putative damaging effects of elevated radiation and a compensatory response to maintain sufficient functioning mitochondria. In animals outside the CEZ, the expression levels of PGC-1α gene and mtDNA copy number were positively correlated as expected from this gene's prominent role in mitochondrial biogenesis; this PGC-1α-mtDNA copy number association is absent in samples from the CEZ. Our data imply that exposure to radionuclides is associated with altered mitochondrial dynamics, evident in level of mtDNA and mtDNA damage and the level of activity in mitochondrial synthesis.

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“…To investigate the cellular responses to UVB, several models have been used including monolayer cells, skin equivalent, skin explant, and mouse models (Cario‐Andre et al, 2000; Cario‐Andre et al, 2002; Duval et al, 2001; Mahfouf et al, 2019; Raad et al, 2017; Rezvani et al, 2006; Rezvani et al, 2007; Scott, 2002). Besides mouse as the favorite mammalian model, several aquatic species, ranging from crustacean and echinoderm to different species of fish and amphibian, have been used to investigate the effects of UV radiation on development and behavior (Blaustein et al, 1997; Bonaventura et al, 2021; de Quadros et al, 2016; Dong et al, 2007; Hurem et al, 2018; Mahmoud et al, 2009; Nahon et al, 2009; Sayed et al, 2013; Sayed et al, 2016; Smith et al, 2000). Of note, increasing UVB radiation due to ozone layer depletion and consequently persistent UVB‐induced damage have been proposed as a potential cause for the disappearance of amphibian populations (Blaustein & Belden, 2003).…”

Section: Introductionmentioning

confidence: 99%

Xenopus: An in vivo model for studying skin response to ultraviolet B irradiation

et al. 2023

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Ultraviolet B (UVB) in sunlight cause skin damage, ranging from wrinkles to photoaging and skin cancer. UVB can affect genomic DNA by creating cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidine (6-4) photoproducts (6-4PPs). These lesions are mainly repaired by the nucleotide excision repair (NER) system and by photolyase enzymes that are activated by blue light. Our main goal was to validate the use of Xenopus laevis as an in vivo model system for investigating the impact of UVB on skin physiology. The mRNA expression levels of xpc and six other genes of the NER system and CPD/6-4PP photolyases were found at all stages of embryonic development and in all adult tissues tested. When examining Xenopus embryos at different time points after UVB irradiation, we observed a gradual decrease in CPD levels and an increased number of apoptotic cells, together with an epidermal thickening and an increased dendricity of melanocytes. We observed a quick removal of CPDs when embryos are exposed to blue light versus in the dark, confirming the efficient activation of photolyases. A decrease in the number of apoptotic cells and an accelerated return to normal proliferation rate was noted in blue light-exposed embryos compared with their control counterparts. Overall, a gradual decrease in CPD levels, detection of apoptotic cells, thickening of epidermis, and increased dendricity of melanocytes, emulate human skin responses to UVB and support Xenopus as an appropriate and alternative model for such studies.

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Developmental effects of exposure to ultraviolet B radiation on the freshwater prawn Macrobrachium olfersi: Mitochondria as a target of environmental UVB radiation

Cited by 16 publications

References 53 publications

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Exposure to environmental radionuclides alters mitochondrial DNA maintenance in a wild rodent

Xenopus: An in vivo model for studying skin response to ultraviolet B irradiation

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