Developmental expression and distribution of opioid receptors in zebrafish

Sánchez-Simón, Fátima Macho; Rodrı́guez, Raquel E.

doi:10.1016/j.neuroscience.2007.09.086

Cited by 43 publications

(47 citation statements)

References 54 publications

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“…9,10 Finally, spatiotemporal expression of the zebrafish opioid receptors shows a wide distribution in the central nervous system at 24 and 48 hpf. 11,12 The early establishment of the main neurotransmission systems before and by the time of PMR initiation is in accordance with the phenotypical clusters we could detect. Moreover, the early aminergic innervation of the spinal cord by the hindbrain, which is described in a study by McLean and Fetcho, 9 is in line with the sudden shift in motor behavior from low-frequency touch responses until 26 hpf to high-frequency swimming from 28 hpf onward.…”

Section: Discussionsupporting

confidence: 86%

A KNIME-Based Analysis of the Zebrafish Photomotor Response Clusters the Phenotypes of 14 Classes of Neuroactive Molecules

Copmans

Meinl²,

Dietz

et al. 2016

SLAS Discovery

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Recently, the photomotor response (PMR) of zebrafish embryos was reported as a robust behavior that is useful for high-throughput neuroactive drug discovery and mechanism prediction. Given the complexity of the PMR, there is a need for rapid and easy analysis of the behavioral data. In this study, we developed an automated analysis workflow using the KNIME Analytics Platform and made it freely accessible. This workflow allows us to simultaneously calculate a behavioral fingerprint for all analyzed compounds and to further process the data. Furthermore, to further characterize the potential of PMR for mechanism prediction, we performed PMR analysis of 767 neuroactive compounds covering 14 different receptor classes using the KNIME workflow. We observed a true positive rate of 25% and a false negative rate of 75% in our screening conditions. Among the true positives, all receptor classes were represented, thereby confirming the utility of the PMR assay to identify a broad range of neuroactive molecules. By hierarchical clustering of the behavioral fingerprints, different phenotypical clusters were observed that suggest the utility of PMR for mechanism prediction for adrenergics, dopaminergics, serotonergics, metabotropic glutamatergics, opioids, and ion channel ligands.

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Section: Discussionsupporting

confidence: 86%

A KNIME-Based Analysis of the Zebrafish Photomotor Response Clusters the Phenotypes of 14 Classes of Neuroactive Molecules

Copmans

Meinl²,

Dietz

et al. 2016

SLAS Discovery

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show abstract

“…Molecularly, the zebrafish opioid receptors and the peptide precursors share close identity with those from humans, mice, or rats [35]. There are both body and brain mean weight loss, after receiving long-term food containing TH, but the relationship between loss of weight and tramadol administration has been seldom documented.…”

Section: Discussionmentioning

confidence: 99%

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Zhuo

Huang

et al. 2012

Journal of Proteomics

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“…Behavioral assays define changes induced by drug exposure and enable differential molecular analysis of the resulting transcriptional (i.e., gene expression) and epigenetic states (Kane, Konu, Ma, & Li, 2004;Kumar et al, 2005;Levine et al, 2005;Nestler, 2008;Renthal et al, 2007;Romieu et al, 2008;Shen et al, 2008). As the reinforcing properties of addictive drugs are highly conserved, behavioral assays have been adapted to zebrafish to study addiction to cocaine (Darland & Dowling, 2001), ethanol (Lockwood, Bjerke, Kobayashi, & Guo, 2004;Peng et al, 2009), amphetamines (Ninkovic & Bally-Cuif, 2006;Webb et al, 2009), and opiates (Bretaud et al, 2007;Lau, Bretaud, Huang, Lin, & Guo, 2006;Sanchez-Simon & Rodriguez, 2008). A broad perspective review of zebrafish use in the study of addiction and other neuropsychiatric disorders has been published (Mathur & Guo, 2010).…”

Section: Drug Addiction and Dependencementioning

confidence: 99%

Zebrafish for the Study of the Biological Effects of Nicotine

Klee

Ebbert

Schneider

et al. 2011

Nicotine & Tobacco Research

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Introduction: Zebrafish are emerging as a powerful animal model for studying the molecular and physiological effects of nicotine exposure. The zebrafish have many advantageous physical characteristics, including small size, high fecundity rates, and externally developing transparent embryos. When combined with a battery of molecular-genetic tools and behavioral assays, these attributes enable studies to be conducted that are not practical using traditional animal models. Methods:We reviewed the literature on the application of the zebrafish model as a preclinical model to study the biological effects of nicotine exposure. Results:The identified studies used zebrafish to examine the effects of nicotine exposure on early development, addiction, anxiety, and learning. The methods used included green fluorescent protein-labeled proteins to track in vivo nicotine-altered neuron development, nicotine-conditioned place preference, and locomotive sensitization linked with high-throughput molecular and genetic screens and behavioral models of learning and stress response to nicotine. Data are presented on the complete homology of all known human neural nicotinic acetylcholine receptors in zebrafish and on the biological similarity of human and zebrafish dopaminergic signaling.Conclusions: Tobacco dependence remains a major health problem worldwide. Further understanding of the molecular effects of nicotine exposure and genetic contributions to dependence may lead to improvement in patient treatment strategies. While there are limitations to the use of zebrafish as a preclinical model, it should provide a valuable tool to complement existing model systems. The reviewed studies demonstrate the enormous opportunity zebrafish have to advance the science of nicotine and tobacco research.

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Developmental expression and distribution of opioid receptors in zebrafish

Cited by 43 publications

References 54 publications

A KNIME-Based Analysis of the Zebrafish Photomotor Response Clusters the Phenotypes of 14 Classes of Neuroactive Molecules

A KNIME-Based Analysis of the Zebrafish Photomotor Response Clusters the Phenotypes of 14 Classes of Neuroactive Molecules

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Zebrafish for the Study of the Biological Effects of Nicotine

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