Developmental expression of B cell molecules in equine lymphoid tissues

Prieto, Jennifer M.; Tallmadge, Rebecca L.; Felippe, M. Julia B.

doi:10.1016/j.vetimm.2016.12.004

Cited by 13 publications

(3 citation statements)

References 104 publications

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“…There are three major subsets of B cells, B1 B cells, marginal zone (MZ) B cells and follicular (FO) B cells (Hoffman et al, 2016;Sebina and Pepper, 2018;Cyster and Allen, 2019), which are also present in horse PBMC (Prieto et al, 2017). Similarly, in this study, genes associated with B1 B cell homeostasis and MZ B cell differentiation (Biological process) were up-regulated during all of the rAHSV4 proteins primary and secondary immune responses (Fig.…”

Section: B Cell Responsessupporting

confidence: 69%

The impact of Escherichia coli contamination products present in recombinant African horse sickness virus serotype 4 proteins on the innate and humoral immune responses

Faber

Tshilwane

Kleef

et al. 2022

Molecular Immunology

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Section: B Cell Responsessupporting

confidence: 69%

The impact of Escherichia coli contamination products present in recombinant African horse sickness virus serotype 4 proteins on the innate and humoral immune responses

Faber

Tshilwane

Kleef

et al. 2022

Molecular Immunology

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“…IGHM transcripts were readily detectable in the KLH-sorted and influenza-sorted B cells of all foals, regardless of group. This finding suggests that the equine neonate produces in utero a repertoire of B cells with Ig variable regions that bind to diverse antigens, including KLH and influenza proteins, and these cells may reflect low-affinity polyreactive natural IgM, perhaps generated by the B-1 cells prevalent in fetal and neonatal periods [ 61 , 62 ]. In addition, a proportion of IgM-expressing B cells are spared from the Ig hypermutation and selection mechanisms performed in lymphoid tissue germinal centers that improve Ig affinity before their isotype switching into IgG [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate

et al. 2017

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The value of prophylactic neonatal vaccination is challenged by the interference of passively transferred maternal antibodies and immune competence at birth. Taken our previous studies on equine B cell ontogeny, we hypothesized that the equine neonate generates a diverse immunoglobulin repertoire in response to vaccination, independently of circulating maternal antibodies. In this study, equine neonates were vaccinated with 3 doses of keyhole limpet hemocyanin (KLH) or equine influenza vaccine, and humoral immune responses were assessed using antigen-specific serum antibodies and B cell Ig variable region sequencing. An increase (p<0.0001) in serum KLH-specific IgG level was measured between days 21 and days 28, 35 and 42 in vaccinated foals from non-vaccinated mares. In vaccinated foals from vaccinated mares, serum KLH-specific IgG levels tended to increase at day 42 (p = 0.07). In contrast, serum influenza-specific IgG levels rapidly decreased (p≤0.05) in vaccinated foals from vaccinated mares within the study period. Nevertheless, IGHM and IGHG sequences were detected in KLH- and influenza- sorted B cells of vaccinated foals, independently of maternal vaccination status. Immunoglobulin nucleotide germline identity, IGHV gene usage and CDR length of antigen-specific IGHG sequences in B cells of vaccinated foals revealed a diverse immunoglobulin repertoire with isotype switching that was comparable between groups and to vaccinated mares. The low expression of CD27 memory marker in antigen-specific B cells, and of cytokines in peripheral blood mononuclear cells upon in vitro immunogen stimulation indicated limited lymphocyte population expansion in response to vaccine during the study period.

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“…Immune cell populations undergo marked expansion in early life before settling to levels found in adult horses [ 33 ]. Similar to human neonates, foal peripheral blood mononuclear cells (PBMCs) are comprised of fewer DCs (CD14 - CD1b + CD86 + ), more regulatory T cells (CD4 + CD25 high FoxP3 + ), and more B1-like CD5 hi cells than adult PBMCs [ 34 – 37 ]. Toll-like receptors are expressed by foal APCs, and IL12p40 and IL12p35 expression is inducible when foal DCs are infected by Rhodococcus equi ; yet, TLR stimulation may not modulate cytokine expression [ 37 – 42 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

et al. 2018

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During the neonatal period, the ability to generate immune effector and memory responses to vaccines or pathogens is often questioned. This study was undertaken to obtain a global view of the natural differences in the expression of immune genes early in life. Our hypothesis was that transcriptome analyses of peripheral blood mononuclear cells (PBMCs) of foals (on day 1 and day 42 after birth) and adult horses would show differential gene expression profiles that characterize natural immune processes. Gene ontology enrichment analysis provided assessment of biological processes affected by age, and a list of 897 genes with ≥2 fold higher (p<0.01) expression in day 42 when compared to day 1 foal samples. Up-regulated genes included B cell and T cell receptor diversity genes; DNA replication enzymes; natural killer cell receptors; granzyme B and perforin; complement receptors; immunomodulatory receptors; cell adhesion molecules; and cytokines/chemokines and their receptors. The list of 1,383 genes that had higher (p<0.01) expression on day 1 when compared to day 42 foal samples was populated by genes with roles in innate immunity such as antimicrobial proteins; pathogen recognition receptors; cytokines/chemokines and their receptors; cell adhesion molecules; co-stimulatory molecules; and T cell receptor delta chain. Within the 742 genes with increased expression between day 42 foal and adult samples, B cell immunity was the main biological process (p = 2.4E-04). Novel data on markedly low (p<0.0001) TLR3 gene expression, and high (p≤0.01) expression of IL27, IL13RA1, IREM-1, SIRL-1, and SIRPα on day 1 compared to day 42 foal samples point out potential mechanisms of increased susceptibility to pathogens in early life. The results portray a progression from innate immune gene expression predominance early in life to adaptive immune gene expression increasing with age with a putative overlay of immune suppressing genes in the neonatal phase. These results provide insight to the unique attributes of the equine neonatal and young immune system, and offer many avenues of future investigation.

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Developmental expression of B cell molecules in equine lymphoid tissues

Cited by 13 publications

References 104 publications

The impact of Escherichia coli contamination products present in recombinant African horse sickness virus serotype 4 proteins on the innate and humoral immune responses

The impact of Escherichia coli contamination products present in recombinant African horse sickness virus serotype 4 proteins on the innate and humoral immune responses

Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate

Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

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