Developmental expression of Pitx2c in Xenopus trigeminal and profundal placodes

Jeong, Yeon Ho; Park, Byung Keon; Saint-Jeannet, Jean Pierre; Lee, Young Ho

doi:10.1387/ijdb.140254js

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…Therefore, we investigated whether neurogenesis was altered in vgll3-depleted embryos using markers of early trigeminal placode and the postmitotic neuronal marker N-tubulin . In stage 14 embryos, vgll3 expression does not colocalize with expression of the trigeminal placode genes islet1 , neuroD , pax3 and foxi1c ( Jeong et al, 2014 ), while in stage 20 embryos, their expression domains become closer ( ).…”

Section: Resultsmentioning

confidence: 99%

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration

et al. 2017

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Drosophila Vestigial is the founding member of a protein family containing a highly conserved domain, called Tondu, which mediates their interaction with members of the TEAD family of transcription factors (Scalloped in Drosophila). In Drosophila, the Vestigial/Scalloped complex controls wing development by regulating the expression of target genes through binding to MCAT sequences. In vertebrates, there are four Vestigial-like genes, the functions of which are still not well understood. Here, we describe the regulation and function of vestigial-like 3 (vgll3) during Xenopus early development. A combination of signals, including FGF8, Wnt8a, Hoxa2, Hoxb2 and retinoic acid, limits vgll3 expression to hindbrain rhombomere 2. We show that vgll3 regulates trigeminal placode and nerve formation and is required for normal neural crest development by affecting their migration and adhesion properties. At the molecular level, vgll3 is a potent activator of pax3, zic1, Wnt and FGF, which are important for brain patterning and neural crest cell formation. Vgll3 interacts in the embryo with Tead proteins but unexpectedly with Ets1, with which it is able to stimulate a MCAT driven luciferase reporter gene. Our findings highlight a critical function for vgll3 in vertebrate early development.

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Section: Resultsmentioning

confidence: 99%

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration

et al. 2017

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“…1I). We compared the expression of cyp26c1 with that of pitx2c, which is expressed in a similar region of the ectoderm (Jeong et al, 2014). We found that pitx2c expression is confined to this crescent-shaped domain and temporally precedes cyp26c1 in this region that later maps to the prospective cement gland (Fig.…”

Section: Cyp26c1 Is Expressed Anteriorly and Is A Target Of Zic1mentioning

confidence: 95%

“…To understand the developmental dynamics of cyp26c1 expression, we used qRT-PCR to compare its temporal expression to that of six1, a PPR gene, and of pitx2c (Jeong et al, 2014), a transcription factor that is a putative Zic1 target (Bae et al, 2014;Jaurena et al, 2015). cyp26c1 is activated shortly after mid-blastula transition and progressively increases until stage 22, followed by a decrease in expression over time (Fig.…”

Section: Cyp26c1 Is Expressed Anteriorly and Is A Target Of Zic1mentioning

confidence: 99%

“…Whole-mount in situ hybridization (Saint-Jeannet, 2017) was carried out in 4 ml glass vials on a standard bench top nutator. Zic1 (Mizuseki et al, 1998), Foxi4.1 (Schlosser andAhrens, 2004;Pohl et al, 2002), Six1 (Pandur and Moody, 2000), Snai2 (Mayor et al, 1995), Dmrta1 (Huang et al, 2005), Hnf1b (Demartis et al, 1994), Sox2 (Mizuseki et al, 1998), Pitx2c (Jeong et al, 2014), Cyp26c1 (Tanibe et al, 2008) and Fgf8a (Christen and Slack, 1997) digoxygenin (DIG)-or fluorescein isothiocyanate (FITC)-labeled antisense RNA probes were synthesized using the Genius Kit (Roche, 1175025). The hybridization step was performed overnight at 60°C.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

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Retinoic acid production, regulation and containment through Zic1, Pitx2c and Cyp26c1 control cranial placode specification

Dubey

Liu

et al. 2021

Development

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All paired sensory organs arise from a common precursor domain called the pre-placodal region (PPR). In Xenopus, Zic1 non-cell autonomously regulates PPR formation by activating retinoic acid (RA) production. Here we identified two Zic1 targets, the RA catabolizing enzyme Cyp26c1 and the transcription factor Pitx2c, expressed in the vicinity of the PPR as crucially required for maintaining low RA levels in a spatially restricted, PPR-adjacent domain. Morpholino- or CRISPR/Cas9-mediated Cyp26c1 knockdown abrogated PPR gene expression, yielding defective cranial placodes. Direct measurement of RA levels revealed that this is mediated by a mechanism involving excess RA accumulation. Furthermore, we show that pitx2c is activated by RA and required for Cyp26c1 expression in a domain-specific manner through induction of FGF8. We propose that Zic1 anteriorly establishes a program of RA containment and regulation through activation of Cyp26c1 and Pitx2c that cooperates to promote PPR specification in a spatially restricted domain.

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Anterior patterning genes induced by Zic1 are sensitive to retinoic acid and its metabolite, 4‐oxo‐RA

Dubey

Saint-Jeannet

2021

Developmental Dynamics

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Background: Development of paired sensory organs is a highly complex and coordinated process. These organs arise from ectodermal thickenings in the cephalic region known as cranial placodes. We have previously shown that Zic1 is a critical regulator for the formation of the pre-placodal region (PPR), the common territory for the development of all cranial placodes in Xenopus laevis.Results: In this study, we have analyzed a number of Zic1 targets for their expression during PPR patterning, as well as their regulation by retinoic acid (RA) and one of its major metabolites, 4-oxo-RA. Our findings show that anteriorly Zic1 regulates several transcription factors, Crx, Fezf2, Nkx3-1, and Xanf1 as well as a serine/threonine/tyrosine kinase, Pkdcc.2. These factors are all expressed in the vicinity of the PPR and as such are candidate regulators of placode formation downstream of Zic1. In addition to their differential regulation by RA, we find that 4-oxo-RA is also capable of modulating the expression of these genes, as well as a broad array of RA-regulated genes. Conclusion: Our data highlight the complexity of retinoid-mediated regulation required for Zic1-activated anterior structure specification in Xenopus, and the potential physiological role of 4-oxo-RA in cranial placode development.

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Developmental expression of Pitx2c in Xenopus trigeminal and profundal placodes

Cited by 3 publications

References 23 publications

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration

Vestigial-like 3 is a novel Ets1 interacting partner and regulates trigeminal nerve formation and cranial neural crest migration

Retinoic acid production, regulation and containment through Zic1, Pitx2c and Cyp26c1 control cranial placode specification

Anterior patterning genes induced by Zic1 are sensitive to retinoic acid and its metabolite, 4‐oxo‐RA

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