Developmental eye motion plasticity after unilateral embryonic ear removal in Xenopus laevis

Gordy, Clayton

doi:10.1016/j.isci.2022.105165

Cited by 2 publications

(18 citation statements)

References 74 publications

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“…2 a , b ; slope = 0.92 ± 0.01, mean ± SD) and showed a gain of 0.28 ± 0.11 ( Fig. 2 a , b , blue, eye motion amplitude/stimulus motion amplitude) in line with previous studies in this species ( Gordy and Straka, 2022 ). To better understand the individual contributions of each eye to these bilateral control visuomotor behaviors, we next investigated OKR performance following acute loss of visual input on one side.…”

Section: Resultssupporting

confidence: 90%

“…One-eyed tadpoles were generated by removal of the left optic vesicle in early embryonic stages, similar to embryonic removal of the otic placode in previous studies ( Fig. 1 a ; stages 26–28; Nieuwkoop and Faber, 1994 ; Gordy and Straka, 2022 ).…”

Section: Resultsmentioning

confidence: 91%

“…Multiple studies back up the second hypothesis. First, embryonically generated one-eared animals compensate in a similar fashion in vestibular processing: At the cost of the normally preferred ipsiversive direction, the normally weak contraversive motion detection is increased to facilitate bidirectional motion sensing ( Gordy and Straka, 2022 ). Second, multiple studies suggest that homeostasis is a common occurrence in tadpole plasticity, rather than response maximization.…”

Section: Discussionmentioning

confidence: 99%

“…1 a , Movie 1 ) with tungsten needles (0.125 mm, Fine Science Tools, 10130-05). Embryos were transferred into 1× MMR at 22°C and anaesthetized with 0.02% benzocaine (Sigma-Aldrich, E1501; Gordy and Straka, 2022 ) prior to the surgical manipulations. The optic vesicle was visually identified, the overlaying ectoderm layer removed, and the optic vesicle excised along with the underlying optic stalk.…”

Section: Methodsmentioning

confidence: 99%

“…Dots that are connected to each other with lines indicate paired data. Circular statistics were performed in Oriana (version 4.02; Kovach Computing Services; Gordy and Straka, 2022 ). A mean vector was calculated from phase values yielding the mean direction as well as the strength of the vector as an indication of data clustering (scale 0–1).…”

Section: Methodsmentioning

confidence: 99%

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Bilateral Retinofugal Pathfinding Impairments Limit Behavioral Compensation in Near-Congenital One-EyedXenopus laevis

Forsthofer,

Gordy,

Kolluri

et al. 2023

eNeuro

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To generate a coherent visual percept, information from both eyes must be appropriately transmitted into the brain, where binocular integration forms the substrate for visuomotor behaviors. To establish the anatomical substrate for binocular integration, the presence of bilateral eyes and interaction of both optic nerves during retinotectal development play a key role. However, the extent to which embryonic monocularly derived visual circuits can convey visuomotor behaviors is unknown. In this study, we assessed the retinotectal anatomy and visuomotor performance of embryonically generated one-eyed tadpoles. In one-eyed animals, the axons of retinal ganglion cells from the singular remaining eye exhibited striking irregularities in their central projections in the brain, generating a non-canonical ipsilateral retinotectal projection. This data is indicative of impaired pathfinding abilities. We further show that these novel projections are correlated with an impairment of behavioral compensation for the loss of one eye.Significance StatementThis study suggests that embryonic sensory imbalance has more far-reaching effects on function compared to acute loss in already established circuits. More generally, bilaterality of sensory organs is the norm across all vertebrates as well as many invertebrates, and our results contribute to a general understanding of how developmental programs, as well as behaviours reliant on them, pivotally depend on the presence of sensory inputs from both body sides.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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