Developmental genetics of hexokinase isozymes in Drosophila melanogaster

Madhavan, Kornath; Fox, David J.; Ursprung, Heinrich W.

doi:10.1016/0022-1910(72)90231-4

Cited by 23 publications

(6 citation statements)

References 20 publications

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“…Previous studies have shown that genes involved in glycolysis are up-regulated at the end of embryogenesis, and that the encoded enzymes become active just prior to the onset of larval development (Wright and Shaw, 1970; Madhavan et al, 1972; Sun et al, 1988; Shaw-Lee et al, 1991; Roselli-Rehfuss et al, 1992; Shaw-Lee et al, 1992; Currie and Sullivan, 1994a; Currie and Sullivan, 1994b; Abu-Shumays and Fristrom, 1997). The observation that these genes are dependent on dERR function raises the possibility that this receptor coordinates their expression at the end of embryogenesis, directing a metabolic switch that promotes carbohydrate metabolism.…”

Section: Resultsmentioning

confidence: 99%

“…It has long been known that many glycolytic enzymes are induced at the onset of larval development, and that the PPP and Ldh are highly active at this stage (Wright and Shaw, 1970; Madhavan et al, 1972; Cochrane et al, 1983; Sun et al, 1988; Shaw-Lee et al, 1991; Roselli-Rehfuss et al, 1992; Shaw-Lee et al, 1992; Currie and Sullivan, 1994a; Currie and Sullivan, 1994b; Abu-Shumays and Fristrom, 1997). No evidence, however, has tied these pathways together.…”

Section: Discussionmentioning

confidence: 99%

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The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth

et al. 2011

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Summary Metabolism must be coordinated with development to provide the appropriate energetic needs for each stage in the life cycle. Little is know, however, about how this temporal control is achieved. Here we show that the Drosophila ortholog of the Estrogen-Related Receptor (ERR) family of nuclear receptors directs a critical metabolic transition during development. dERR mutants die as larvae with low ATP levels and elevated levels of circulating sugars. The expression of active dERR protein in mid-embryogenesis triggers a coordinate switch in gene expression that drives a metabolic program normally associated with proliferating cells, supporting the dramatic growth that occurs during larval development. This study shows that dERR plays a central role in carbohydrate metabolism, demonstrates that a proliferative metabolic program is used in normal developmental growth, and provides a molecular context to understand the close association between mammalian ERR family members and cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth

et al. 2011

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“…Except for hexokinase, most of the Drosophila melanogaster enzymes of the glycolytic pathway are present as a single isoform encoded by a single locus. In D. melanogaster three forms of hexokinases (HEX‐A, ‐B and ‐C) have been reported by zymographic studies using agar gel electrophoresis (Madhavan et al ., 1972). All three forms are monomers with molecular mass ranging from 40 000 to 50 000.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of Drosophila melanogaster hexokinase Hex‐A locus: multiple Initiator‐like elements enhance DPE containing promoter activity

Jayakumar¹,

Shouche²,

Patole³

2006

Insect Molecular Biology

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Flight muscle Hexokinase-A (HEX-A) is the most conserved and essential hexokinase isoenzyme among Drosophila species. In this study, the Hex-A locus, encoding the HEX-A isoenzyme, has been analysed for the elements regulating its expression. By sequencing the 5' ends of Hex-A cDNA amplified by 5' RACE, we identified a transcription start site that overlapped the Initiator and downstream promoter elements. A 214 bp sequence, encompassing transcription start sites and promoter elements, was required for minimal promoter activity. DNA sequence to the 5' end of the minimal promoter element did not demonstrate any promoter activity; however, its inclusion with the basal promoter element enhanced the promoter activity. Oligonucleotide competition and site-directed mutagenesis identified the Initiator-like sequences, TCAWT, present in this region that were responsible for enhancing the promoter activity. The Hex-A locus is expressed as a single protein in Drosophila cell line, whereas in pupae, larvae and adult flies, it is expressed as two distinct types.

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“…One solution has been the development of genetically independent isozymes via gene duplication (1). The lactate dehydrogenase isozymes in vertebrates and the hexokinase isozymes in Drosophila are the classic examples of such systems (2,3). Alternatively, many single genes are expressed in diverse tissues and developmental stages, after which they are post-transcriptionally modified in a tissue-specific manner (4).…”

mentioning

confidence: 99%

“…Inasmuch as sexual dimorphism presents a complex regulatory problem, it is not surprising that genetically independent isozymes constitute all known sex-limited enzyme systems. Thus testis-specific lactate dehydrogenase X in mammals (2) and testis-specific hexokinase t in Drosophila (3) Electrophoretic Assays. Crude extracts of one or more Gos!…”

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confidence: 99%

Biphasic expression and function of glucose dehydrogenase in Drosophila melanogaster.

Cavener¹,

MacIntyre²

1983

Proc. Natl. Acad. Sci. U.S.A.

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Glucose dehydrogenase (GO) was found to be expressed during the pupal stage in both sexes of Drosophila melanogaster, but is limited to the male ejaculatory duct at the adult stage. During copulation GO is transferred from males to females. Mutational analysis of the Go locus indicates that a single structural gene encodes the pupal and ejaculatory duct GO. Thus an example of an enzyme structural gene switching from non-sexlimited to sex-limited expression has been found. Go mutants are recessive lethals exhibiting a late pupal effective lethal phase. These mutants can be rescued by excising the anterior end of the pupal case 0-2 days prior to the normal adult emergence time. It appears that the function of GO in pupae is to aid in the degradation of the puparium cuticle in preparation for the eclosion of the adult.

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Developmental genetics of hexokinase isozymes in Drosophila melanogaster

Cited by 23 publications

References 20 publications

The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth

The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth

Functional analysis of Drosophila melanogaster hexokinase Hex‐A locus: multiple Initiator‐like elements enhance DPE containing promoter activity

Biphasic expression and function of glucose dehydrogenase in Drosophila melanogaster.

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