Developmental pathway of granular and beaded nuclear bodies from nucleoli

Dupuy-Coin, A.M.; Bouteille, M.

doi:10.1016/s0022-5320(72)80022-4

Cited by 46 publications

(7 citation statements)

References 27 publications

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“…However, Zu Rhein and Chou (1968) have expressed some reservations. Moreover, these bodies seem to be more common in non-pathological tissues than was generally appreciated in the past (Dupuy-Coin and Bouteille, 1972). Recently, Feldman and Peters (1972) have noted an increase in their fre- cytoplasmic aggregates quency in normal ageing rats and associated them with heightened cellular activity.…”

Section: Discussionmentioning

confidence: 92%

Cytoplasmic Tubular Aggregates and Nuclear Filamentous Bodies in Two Suspected Cases of Viral Encephalitis

Horoupian

Ross

Gurwith

et al. 1974

Can. j. neurol. sci.

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Section: Discussionmentioning

confidence: 92%

Cytoplasmic Tubular Aggregates and Nuclear Filamentous Bodies in Two Suspected Cases of Viral Encephalitis

Horoupian

Ross

Gurwith

et al. 1974

Can. j. neurol. sci.

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“…PIKAs show less resemblance to nuclear bodies, which are spherical, encapsulated structures that apparently bud off of the nucleolus and may contain nascent ribosomal RNA (Vagner-Capodano et al, 1982) . The structure of nuclear bodies varies considerably: both the granular and fibrillar components ofthe nucleolus can be seen in different proportions for different nuclear bodies (Dupuy-Coin and Bouteille, 1972). Autoantibodies that recognize nuclear bodies have recently been described (Fusconi et al, 1991) .…”

Section: Compartmentalization Within the Nucleusmentioning

confidence: 99%

Compartmentalization within the nucleus: discovery of a novel subnuclear region.

Saunders

Cooke

Earnshaw

1991

The Journal of Cell Biology

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. Antibodies to a set of structurally related autoantigens (p23-25) bind to a previously uncharacterized, large structural domain in the nucleus of a variety of human cell types. This subnuclear domain is visible by phase contrast alone as a region of decreased density after several different fixation protocols . The morphology of this region changes dramatically during the cell cycle and we have given it the name PIKA (for polymorphic interphase karyosomal association) based on preliminary evidence that the PIKA proteins may be associated with chromatin . The function of the PIKA is not yet known, but our immunolocalization B EYOND such obvious features as the nucleolus, heterochromatin, and the nuclear membrane, the structural organization of the nucleus is surprisingly poorly understood . While many aspects of nuclear physiology have proven amenable to analysis in vitro, a complete understanding of the cellular control of nuclear processes, which include transcription, nascent RNA splicing and processing, and transport of mature mRNA, may ultimately depend on our knowledge of the organization of the nucleoplasmic material surrounding the interphase chromosomes. At present, much about the organization ofthe nucleoplasm remains unclear. Specifically, it is unknown to what extent the nucleoplasm is functionally and structurally partitioned into separate domains .Efforts to understand the structure of the nucleoplasm by biochemical fractionation have met with only limited success. Many attempts at separating the nucleus into its constituent substructures have involved disruptive procedures such as nuclease digestion and/or detergent and salt extraction (Berezney and Coffey, 1975;Berezney, 1984;Vogelstein et al ., 1985) . Biochemical fractionation procedures have permitted identification of proteins associated with small nuclear RNA (snRNA) ' (Lerner and Steitz, 1979; Pettersson W . S. Saundees present address

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“…Indeed, inactive granulated re sidual nucleoli have been observed in the mouse oo cyte (Mirre and Stahl, 1978). Finally, it could belong to a poorly defined category of " nuclear-bodies" (Dupuy-Coin and Bouteille, 1972;Vagner-Capodano et al, 1980). It is known that some of these nuclear bodies are the result of nucleolar emissions (VagnerCapodano et al, 1980), whereas others are probably formed elsewhere in the genome (Le Goascogne and Beaulieu, 1977).…”

Section: Discussionmentioning

confidence: 99%

Nucleoli, micronucleoli, and nucleolus-like structures in human oocytes at meiotic prophase I studied by the silver-NOR technique

Hartung¹,

Keeling

Patel

et al. 1983

Cytogenet Genome Res

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Meiotic nuclei preparations obtained from human fetal ovaries were studied with the silver-NOR technique. At leptotene, the NOR’s were located at the periphery of the nucleoli. The mean number of NOR’s per nucleus was 6.4. Moreover, 2–12 micronucleoli, each containing an Ag-positive zone, were observed. At pachytene, each nucleolus was divided into two zones, one of which was Ag-positive. The mean number of Ag-positive zones was 2.1 per nucleus. The subterminal region of the short arm of nucleolar bivalents was in contact with the argyrophilic nucleolar zone. In most cells, micronucleoli were dispersed between the bivalents. A single rounded body which stained heavily with silver was consistently observed. At early diplotene, the NOR’s separated, appearing again as distinct rounded Ag-positive structures embedded in the peripheral part of the nucleoli. The silver-stained round body was still visible. Eight to twelve micronucleoli were observed. At a more advanced stage of diplotene, the number of micronucleoli increased, varying from 20 to 50 per nucleus. These observations suggest that the micronucleoli, already visible at the leptotene stage, are the morphological expression of an early transcription of amplified rDNA. The biological significance of the Ag-positive round body remains obscure, but the consistency of its appearance suggests that it has a specific function somehow related to nucleolar proteins.

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Developmental pathway of granular and beaded nuclear bodies from nucleoli

Cited by 46 publications

References 27 publications

Cytoplasmic Tubular Aggregates and Nuclear Filamentous Bodies in Two Suspected Cases of Viral Encephalitis

Cytoplasmic Tubular Aggregates and Nuclear Filamentous Bodies in Two Suspected Cases of Viral Encephalitis

Compartmentalization within the nucleus: discovery of a novel subnuclear region.

Nucleoli, micronucleoli, and nucleolus-like structures in human oocytes at meiotic prophase I studied by the silver-NOR technique

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