Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts

Abstract

ABSTRACT:The present study was aimed to investigate the developmental patterns of FASN (fatty acid synthase) and LIPE (lipase, hormone-sensitive) mRNA in adipose tissue in pigs of different breeds and the relation with carcass fat content. Subcutaneous adipose tissue was sampled and total RNA was extracted to determine FASN and LIPE mRNA levels by semi-quantitative RT-PCR. The results showed that the FASN mRNA level increased with age (P < 0.05) and Jinhua gilts expressed higher FASN mRNA compared with Landrace gilts at 80 and 125 days of age (P < 0.05). In addition, Jinhua gilts expressed lower LIPE mRNA compared with Landrace gilts at 80 days of age (P < 0.01). Furthermore, the ratio of FASN/LIPE mRNA had a similar model in the two breeds, and was higher in Jinhua gilts than that in Landrace gilts at 80 and 125 days of age (P < 0.05). The FASN mRNA level was positively related to carcass fat content in Jinhua and Landrace gilts (r = 0.802, P = 0.01; r = 0.734, P = 0.02; respectively), and the ratio of FASN/LIPE expression exhibited significantly positively related carcass fat content (r = 0.804, P = 0.01; r = 0.749, P = 0.02; respectively).

Expression analyses of candidate genes related to meat quality traits in squabs from two breeds of meat‐type pigeon

“…Consistent with previous studies performed by Ponsuksili, Murani, Walz, Schwerin, and Wimmers () and Miao et al. () on pigs differing in fatness traits, which revealed that the expression of FASN was up‐regulated in the fatter pig breed than in the leaner breed, our present study identified significantly increased expression of FASN in both the liver and the breast muscle in male SQ squabs characterized with significantly higher IMF content. The failure of detection of significant difference in the FASN expression in female squabs between two breeds might be due to by the relatively limited number of samples used in this study and the large between‐animal variance.…”

Effects of maternal vitamin D3 during pregnancy on FASN and LIPE mRNA expression in offspring pigs

“…In this present experiment, we found that the ratio of FASN/LIPE mRNA expression in adipose tissue was positively correlated with carcass fat content and ABFT, which suggested that carcass content and ABFT in pigs were affected by the ratio of FASN/LIPE mRNA expression. Similar results were reported by Miao et al (2010), who demonstrated that there was a positive relationship between the ratio of FASN/LIPE mRNA expression and carcass fat in pigs. Meanwhile, FASN mRNA expression was negatively correlated with AFBT, whereas, there was a positive correlation between the ratio of FASN/LIPE mRNA expression and AFBT.…”

“…These results suggested that lipid synthesis and degradation in offspring were impacted by maternal nutrition. Previous reports demonstrated that adipose tissue deposition depends on the balance between lipid synthesis and degradation (Qiao et al ., 2007; Miao et al ., 2010). This process is mainly regulated by fatty acids synthase ( FASN ) and hormone-sensitive lipase ( LIPE ).…”

Pig fatness in relation to FASN and INSIG2 genes polymorphism and their transcript level

“…ME , SCD and ACACA , which transcript levels were elevated in the SC of PL pigs when compared to other analyzed breeds [ 17 – 19 ]. Interestingly, Miao et al [ 20 ] claimed that the FASN transcript was more abundant in SC of Jinhua pigs (a fat Chinese breed) than in the Landrace pigs (a leaner breed). Furthermore, considering that gilts in our study were kept under identical conditions and slaughtered at the same body weight, one may conclude of a significant genetic impact on observed differences between breeds.…”