Developmental recording of the ghost-moth larvae after ex situ infection by Ophiocordyceps sinensis

Li, Wenjia; Xia, Jinming; Li, Quanping; Zhang, Zongyao; Zhang, Wei-Wei; Dong, Chao; Wei, Jiang-Chun; Liu, Xingzhong

doi:10.1007/s11427-020-1686-7

Cited by 4 publications

(7 citation statements)

References 5 publications

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“…Wildman and Zimmer encouraged discussions of the relevant scientific issues in the journal with the authors (Zhu et al . 2019; Li et al . 2020a).…”

Section: Discussionmentioning

confidence: 99%

“…(2023c) also identified GC-biased Genotype #13 in semiejected ascospores and GC-biased Genotype #14 in fully ejected ascospores collected from the same specimens of natural C. sinensis . Although Li et al . (2020a) amended their inoculant information, the inoculant used in the industrial cultivation project was O. sinensis , which was isolated from the ascospore of Chinese cordyceps ( i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Although Li et al [77] amended their inoculant information, the inoculant used in the industrial cultivation project was O. sinensis, which was isolated from the ascospores of Chinese cordyceps (i.e., natural C. sinensis) collected from an alpine meadow of Nyingchi, Tibet Autonomous Region, China, but not the 3 pure GC-biased Genotype #1 H. sinensis strains reported by Wei et al [20]. Li et al [9,32] reported that the fully ejected ascospores of natural C. sinensis contained GC-biased Genotypes #1 and #14 and AT-biased O. sinensis Genotypes #5-6 and #16 within AT-biased Cluster A (cf.…”

Section: Iv-61 Differential Occurrence Of At-biased Genotypes In Diff...mentioning

confidence: 98%

“…These studies indicated that the ascospores did not contain Genotypes #4 and #15 of AT-biased Cluster B. Thus, Wei et al [20] and Li et al [77] actually left an unsolved puzzle regarding the fungal source of the sole teleomorphic AT-biased Genotype #4 in cultivated C. sinensis.…”

Section: Iv-61 Differential Occurrence Of At-biased Genotypes In Diff...mentioning

confidence: 99%

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Genetic and phylogenetic features of the repetitive ITS copies in theHirsutella sinensisgenome and the genomically independent AT-biasedOphiocordyceps sinensisgenotypes

Wang

Zhu

2022

Preprint

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Li et al. (2013, 2020b) hypothesized that AT-biased genotypes of Ophiocordyceps sinensis are generated through repeat-induced point mutation (RIP) and exist as ITS pseudogenes in a single genome of GC-biased Genotype #1 Hirsutella sinensis. This study revealed multiple GC-biased repetitive ITS copies in the H. sinensis genome, including multiple insertion/deletion and transversion alleles, which might not be generated through RIP mutagenesis that theoretically causes C-to-T and G-to-A transitions. The repetitive ITS copies are genetically and phylogenetically distinct from the AT-biased genotypes of O. sinensis, which possess multiple scattered transition alleles. The sequences of GC- and AT-biased Genotypes #2-17 were absent in the genome assemblies ANOV00000000, JAAVMX000000000, LKHE00000000, LWBQ00000000 and NGJJ00000000 of H. sinensis strains but instead in the genomes of independent O. sinensis fungi. The GC- and AT-biased genotypes of O. sinensis were found to differentially occur in different combinations in different compartments of natural Cordyceps sinensis. Their abundances were dynamically altered in an asynchronous, disproportional manner during C. sinensis maturation. Metatranscriptomic analysis of natural C. sinensis revealed transcriptional silencing of the 5.8S genes of all C. sinensis-colonizing fungi, including H. sinensis. The problematic design of the 5.8S gene transcription assay and data analysis reported by Li et al. (2013) have provided controversial and incomplete evidence to support the notion that the 5.8S genes of AT-biased genotypes of O. sinensis are permanently nonfunctional ITS pseudogenes. These results demonstrate the genomic independence of 17 genotypes belonging to independent O. sinensis fungi. In parallel with GC-biased genotypes, AT-biased O. sinensis genotypes might have been generated during the long history of evolution from a common ancestor through RIP or other mutagenesis mechanisms in response to the extremely harsh environment of the Qinghai-Tibet Plateau.

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“…Wildman and Zimmer encouraged discussions of the relevant scientific issues in the journal with the authors (Zhu et al . 2019; Li et al . 2020a).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Iv-61 Differential Occurrence Of At-biased Genotypes In Diff...mentioning

confidence: 98%

Section: Iv-61 Differential Occurrence Of At-biased Genotypes In Diff...mentioning

confidence: 99%

See 2 more Smart Citations

Genetic and phylogenetic features of the repetitive ITS copies in theHirsutella sinensisgenome and the genomically independent AT-biasedOphiocordyceps sinensisgenotypes

Wang

Zhu

2022

Preprint

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“…Indeed, the Hepialus larva feeds on plant roots [27], and the O. sinensis fungus depends on microbial reserves in the soil [28,29]. After being infected (in the soil) by the fungus, the caterpillar remains alive for a long period (5-12 months in laboratory, may be longer in the wild) and keeps developing until O. sinensis mummi ed [30,31]. Upon its death, the caterpillar moves near the surface of the soil (2-5 cm depth) and places itself vertically, with the head facing upward, allowing the stromata to grow out of the soil and release the ascospores [32].…”

Section: Introductionmentioning

confidence: 99%

Building an Integrative and Testable Hypothesis on How Edaphic Factors Ultimately Influence the Occurrence and Quality of Ophiocordyceps Sinensis, the Vegetable Caterpillar

Deng¹,

Yang²,

Favre³

2021

Preprint

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BackgroundThe yield of commercially harvested “vegetable caterpillar” Ophiocordyceps sinensis has dramatically plummeted in the last few decades, while market demand has increased. Besides controlling the obvious overexploitation of this species, understanding how edaphic factors influence this system may improve the chances of successful cultivation and thus support the conservation of O. sinensis in the wild. Our study investigates how the presence/absence and the quality of O. sinensis may be linked to a series of edaphic factors pertinent to its microhabitat, including enzyme activity, nutrients, moisture, pH and nematode population. In order to provide a preliminary hypothesis on the relationships among edaphic factors and their influence on O. sinensis, we performed a principal component analysis and structural equation modelling despite limited replication. ResultsSoil samples containing O. sinensis were more moist and contained a higher concentration of nutrients and enzyme activity than control samples collected nearby, where the species was absent. Preliminary analyses indicated that enzyme activity may be crucial and appeared to be affected by a number of other soil factors. We found that O. sinensis would occupy microhabitats with a relatively higher soil fertility and a more persistent enzyme activity, where the values of total nitrogen and catalase are especially important. Otherwise, with the exception of organic matter and enzyme activity, mean values did not suggest any other factors potentially corresponding to a better quality of O. sinensis.ConclusionsBased on these preliminary findings and a further literature review, we formulated the first integrative hypothesis (network of interactions) on how soil factors may influence each other and O. sinensis. Finally, we indicate how this hypothesis may be tested in the future, in order to increase the chances for successful cultivation and thus promote the conservation and sustainable harvesting of O. sinensis.

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The intricate dance: Exploring the interactions between entomopathogenic fungi and insects with special focus on the formation/production of Chinese cordyceps

Khalid,

Han

et al. 2024

Fungal Biology Reviews

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Developmental recording of the ghost-moth larvae after ex situ infection by Ophiocordyceps sinensis

Cited by 4 publications

References 5 publications

Genetic and phylogenetic features of the repetitive ITS copies in theHirsutella sinensisgenome and the genomically independent AT-biasedOphiocordyceps sinensisgenotypes

Genetic and phylogenetic features of the repetitive ITS copies in theHirsutella sinensisgenome and the genomically independent AT-biasedOphiocordyceps sinensisgenotypes

Building an Integrative and Testable Hypothesis on How Edaphic Factors Ultimately Influence the Occurrence and Quality of Ophiocordyceps Sinensis, the Vegetable Caterpillar

The intricate dance: Exploring the interactions between entomopathogenic fungi and insects with special focus on the formation/production of Chinese cordyceps

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