1998
DOI: 10.1016/s0176-1617(98)80021-7
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Developmental-regulation and tissue-specific expression of two different seed promoter GUS-fusions in transgenic lines of Vicia narbonensis

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Cited by 15 publications
(4 citation statements)
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“…Afterwards the activity dropped to 0.01 µmol g −1 min −1 , but increased steadily in the wild‐type embryos to around 1 µmol g −1 min −1 at 32 DAP ( Figure 2a). This time course of AGP depression reflects the activity of the LeB4 promoter ( Pickardt et al ., 1998 ). Accordingly, the starch content of as1 and wild‐type embryos was similar up to 100 mg per embryo, but was different during the second part of seed development, when it was decreased by around 40–50% on a fresh weight basis ( Figure 2b).…”
Section: Resultsmentioning
confidence: 99%
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“…Afterwards the activity dropped to 0.01 µmol g −1 min −1 , but increased steadily in the wild‐type embryos to around 1 µmol g −1 min −1 at 32 DAP ( Figure 2a). This time course of AGP depression reflects the activity of the LeB4 promoter ( Pickardt et al ., 1998 ). Accordingly, the starch content of as1 and wild‐type embryos was similar up to 100 mg per embryo, but was different during the second part of seed development, when it was decreased by around 40–50% on a fresh weight basis ( Figure 2b).…”
Section: Resultsmentioning
confidence: 99%
“…Both AGP‐mRNA levels and enzyme activity were similar to the wild type up to 20 DAP and thereafter dropped to very low values. The pattern reflects the activity of the LeB4 promoter, which is mostly active in the cotyledons during the mid‐ to late‐maturation phase ( Pickardt et al ., 1998 ; Weber et al ., 1998a ).…”
Section: Discussionmentioning
confidence: 99%
“…The antisense inhibition of VnGPT1 generated no obvious phenotypic abnormalities, either in the seed coat or in any of the non‐seed organs. The absence of such effects may be because of the negligible level of LeB4‐driven antisense expression in non‐seed tissues (Pickardt et al. , 1998; Zakharov et al.…”
Section: Discussionmentioning
confidence: 99%
“…Histochemical staining was performed as described [ 62 ]. Arabidopsis plants were grown in soil pots under normal conditions.…”
Section: Methodsmentioning
confidence: 99%