Developmental regulation of an acyl carrier protein gene promoter in vegetative and reproductive tissues

Baerson, Scott R.; Lamppa, Gayle K.

doi:10.1007/bf00014933

Cited by 22 publications

(14 citation statements)

References 33 publications

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“…Overall, these studies indicated that leaves and roots require different domains of the Acll.2 gene promoter for maximal expression. Deletion of the -520 to -321 region did not significantly alter Acl 1.2 promoter activity in developing leaves (P<0.5), which was comparable to median activity levels observed in transformant populations containing the -840 to + 74 promoter region found previously [2]. In contrast, removal of the -320 to -236 region resulted in a significant decrease in GUS activity, with median levels observed in A5 transformants about 9-fold lower than those observed in A4-transformed plants.…”

Section: ' Deletion Analysis Of the Acyl Carrier Protein Acll2 Genesupporting

confidence: 66%

“…Previously we showed that Acll.2 gene promoter activity declines approximately 16-fold during leaf maturation in transformed tobacco plants [2]. Interestingly, deletion of the -320 to -236 region resulted in GUS activity levels in young leaves that were nearly the same as those observed in mature leaves containing the fulllength Acll.2 GUS construct (shown for comparison in Fig.…”

Section: ' Deletion Analysis Of the Acyl Carrier Protein Acll2 Genementioning

confidence: 80%

“…5' deletion derivatives of the ACP Ac11.2 gene promoter were prepared from an Ac11.2 promoter-fi-glucuronidase (GUS) chimeric gene containing the -840 to + 74 region positioned upstream of the GUS coding region [2]. The Acll.2-GUS fusion construct was cloned into the Eco RI site of the pIBI31 (International Biotechnologies, New Haven, CT) polylinker, and then digested with Sph I and Barn HI for digestion with Exonuclease III, followed by S 1 nuclease, as described by Ausubel et al [1].…”

Section: Plasmid Constructionmentioning

confidence: 99%

“…To begin to understand how FAS genes are regulated in plants, we previously examined the expression of the promoter from the Arabidopsis ACP Acll.2 gene (originally, the ACP A1 gene [21]; renamed in accordance with the ISPMB Commission on Plant Gene Nomenclature) fused to a reporter gene coding for ~-glucuronidase (GUS) in transgenic tobacco plants [2]. GUS activity was highest in seeds, and increased during their development, de Silva et al [8] showed that one Brassica ACP gene, which is seedspecific, also becomes most active coincident with lipid synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Identification of domains in an Arabidopsis acyl carrier protein gene promoter required for maximal organ-specific expression

1994

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Deletions were made in the promoter of the acyl carrier protein (ACP) Acll.2 gene from Arabidopsis to investigate the nature of the cis-acting elements that direct its expression. These constructs, which included the untranslated leader region, were fused to a reporter gene coding for beta-glucuronidase (GUS) and transformed into tobacco. Quantitative fluorometric analysis of GUS activity in transgenic plants showed that expression in young leaves drops to a basal level when a 85 bp domain, from -320 to -236 relative to transcription initiation, is deleted. Maximum promoter activity in roots also depends on this domain, but two other regions are also important. In total, deletion of the sequences from -466 to -55 caused an ca. 80-fold reduction in Acl1.2 promoter activity in roots. The -320 to -236 domain forms a complex with a protein factor found in leaves and roots, which was not detectable in seeds. The formation of this protein-DNA complex was abolished by mutation of a bZIP core motif, ACGT, found within the context AAGACGTAG, which is dissimilar to the other bZIP-binding sites thus far characterized in plants. Previously we showed that Acl1.2 promoter activity is highest in seeds [2]. Here we find, in contrast to leaves and roots, that deletion to position -236 has no effect on GUS levels in seeds. However, nearly a 100-fold drop was observed when the -235 to -55 region was removed. Hence, this 180 bp domain contains all the cis-acting information necessary for Acl1.2 promoter activity in seeds. The same region is necessary for Acl1.2 activity in the receptacle, stigma, tapetum and pollen of the flower, as demonstrated by histochemical staining.

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Section: ' Deletion Analysis Of the Acyl Carrier Protein Acll2 Genesupporting

confidence: 66%

Section: ' Deletion Analysis Of the Acyl Carrier Protein Acll2 Genementioning

confidence: 80%

Section: Plasmid Constructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of domains in an Arabidopsis acyl carrier protein gene promoter required for maximal organ-specific expression

1994

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“…The segregation (18,28) and chimerism (2,3,4) in the dihaploid lines reveal only part of the reasons for this situatuion. On the other hand, we achieved considerable results with non-segregating DH (8,10,33 (11,12,44,123) etc. To explain the depression of the agronomic performance of the dihaploid lines several hypothesis were raised: -inbreeding depression and residual heterozygosity which seem similar to the phetype suppression after long-term self-fertilization in many cultivated species (5,17,18,19,20,23,114,115,116,117); -cytoplasmic and/or nuclear genes modifications (17,28,51), gross chromosomal changes ( 4 2, 117) or organ ells replication (113) as a result of the in vitro culture; -DNA amplification in comparison to the source genotype (29,30,114 the yield loss recovery could be achieved after several recurrent full-sib selections (148).…”

Section: Introductionmentioning

confidence: 94%

Androgenesis in Vitro in Tobacco

Atanassov¹,

Djilianov²

1997

Biotechnology & Biotechnological Equipment

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Control and Silencing of Transgene Expression

Müller

Wassenegger

2004

Handbook of Plant Biotechnology

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The expression of a gene is a fundamental biological process with both immediate effects on an individual organism as well as long‐term consequences for the evolution of the living world. As such, gene expression is regulated by elaborate control mechanisms that act or interact to ensure a fine context‐specific tuning. Transgenes are subject to the same range of gene regulatory mechanisms that control endogenes. In addition, transgenes, like other newly acquired foreign or ‘invasive’ DNA elements in the genome, appear particularly susceptible to control mechanisms that detect and act upon ‘abnormalities’ encoded by the transgene sequence or structure, the transgene's positional and compositional relation to its genomic environment, the transgene's expression pattern, and the intrinsic and extrinsic characteristics of transgenic RNA intermediates and gene products. A thorough understanding of the pathway of gene expression, from transcriptional regulation by genetic and epigenetic mechanisms, through to RNA and protein degradation, and the targets this pathway provides for gene regulatory processes to intervene, is essential for the successful application of transgenic tools in today's plant biotechnology.

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Developmental regulation of an acyl carrier protein gene promoter in vegetative and reproductive tissues

Cited by 22 publications

References 33 publications

Identification of domains in an Arabidopsis acyl carrier protein gene promoter required for maximal organ-specific expression

Identification of domains in an Arabidopsis acyl carrier protein gene promoter required for maximal organ-specific expression

Androgenesis in Vitro in Tobacco

Control and Silencing of Transgene Expression

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