Developmental regulation of CRD-BP, an RNA-binding protein that stabilizes c-myc mRNA in vitro

Leeds, Peter; Kren, Betsy T.; Boylan, Joan M.; Betz, Natalie A.; Steer, Clifford J.; Gruppuso, Philip A.; Ross, Jeffrey

doi:10.1038/sj.onc.1201093

Cited by 107 publications

(110 citation statements)

References 20 publications

(29 reference statements)

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“…This region of c-myc mRNA interacts with CRD-BP, a protein that shields it from endonucleolytic attack in vitro (Bernstein et al, 1992;Herrick and Ross, 1994). Like AUF1 and HuR, CRD-BP is expressed during liver development but absent in both quiescent adult liver and regenerating liver (Leeds et al, 1997). Both coding region determinants in c-myc mRNA were shown to act in vivo independently of the 3'-UTR instability determinant (Lavenu et al, 1995;Pistoi et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins

et al. 1998

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Several proteins that may regulate c-myc mRNA posttranscriptionally were previously isolated and characterized. Two of them, HuR and AUF1, bind speci®cally to the 3' untranslated region (UTR) of c-myc mRNA. Because c-myc is regulated post-transcriptionally in various mouse tissues, including quiescent tissues, fetal liver and regenerating liver, we investigated whether HuR and AUF1 expression was also regulated in these tissues. Concerning AUF1, we analysed the expression of various mRNA and protein isoforms. We discovered a new AUF1 mRNA variant with a long AU-rich 3' UTR. We show that AUF1 expression, regardless of the RNA isoform considered, and HuR mRNA expression parallel c-myc expression in quiescent tissues and during liver development; their expression is high in lymphoid tissues and fetal liver and low in adult liver. However, no upregulation of HuR or AUF1 accompanies the upregulation of c-myc mRNA following partial hepatectomy. We discuss our results in relation to the current hypothesis that HuR and AUF1 act as mRNA destabilizing factors.

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Section: Discussionmentioning

confidence: 99%

Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins

et al. 1998

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“…However, CRD-BP is also detected in established cell lines of neoplastic and preneoplastic origin, implying an oncofetal pattern of expression. 4,5 Mouse CRD-BP is orthologous to human IGF-II mRNA-binding protein-1 (hIMP-1), which binds IGF-II leader 3 mRNA 6 and is believed to control its translation and subcytoplasmic localization. IGF-II belongs to the family of insulin-like peptides; it promotes cell-cycle progression and differentiation and inhibits apoptosis.…”

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confidence: 99%

C‐MYC and IGF‐II mRNA‐binding protein (CRD‐BP/IMP‐1) in benign and malignant mesenchymal tumors

et al. 2001

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Mouse coding region determinant-binding (mCRD-BP)and human IGF-II mRNA-binding 1 (hIMP-1) proteins are orthologous mRNA-binding proteins that recognize c-myc and IGF-II mRNA, respectively, and regulate their expression posttranscriptionally. Here, we confirm that human CRD-BP/IMP-1 binds to c-myc mRNA and that it is predominantly expressed in fetal tissues. Moreover, hCRD-BP/IMP-1 expression was detected in cell lines of neoplastic origin and in selected primary tumors. In a series of 33 malignant and 10 benign mesenchymal tumors, 73% and 40%, respectively, were found to express hCRD-BP/IMP-1. In particular, expression was significant in 14 Ewing's sarcomas, all of which were positive. The data suggest that hCRD-BP/IMP-1 plays a role in abnormal cell proliferation in mesenchymal tumors.

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“…In this way, they are involved in the temporal and spatial control of gene expression at the level of mRNA rather than at the level of gene transcription. The three mammalian VICKZ proteins (IMP1/CRD-BP, IMP2, and IMP3/KOC) are primarily expressed during embryonic development, and their expression is generally not detected in adult tissues, with the exception of placenta and testes (1)(2)(3)(4). In contrast, they are overexpressed in various cancers (reviewed in refs.…”

Section: Introductionmentioning

confidence: 99%

HMGA2 Regulates Transcription of the Imp2 Gene via an Intronic Regulatory Element in Cooperation with Nuclear Factor-κB

Cleynen

Brants

Peeters

et al. 2007

Molecular Cancer Research

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IMP2 (insulin-like growth factor-II mRNA binding protein 2) is an oncofetal protein that is aberrantly expressed in several types of cancer. We recently identified the Imp2 gene as a target gene of the architectural transcription factor HMGA2 (high mobility group A2) and its tumor-specific truncated form HMGA2Tr. In this study, we investigated the mechanism via which HMGA2 regulates Imp2 gene expression. We show that HMGA2 and HMGA2Tr directly regulate transcription of the Imp2 gene by binding to an AT-rich regulatory region located in the first intron.In reporter experiments, we show that this AT-rich regulatory region mimics the response of the endogenous Imp2 gene to HMGA2 and HMGA2Tr. Furthermore, we show that a consensus nuclear factor-KB (NF-KB) binding site located immediately adjacent to the AT-rich regulatory region binds NF-KB and that NF-KB and HMGA2 cooperate to regulate Imp2 gene expression. Finally, we provide evidence that there is a strong and statistically significant correlation between HMGA2 and IMP2 gene expression in human liposarcomas.

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Developmental regulation of CRD-BP, an RNA-binding protein that stabilizes c-myc mRNA in vitro

Cited by 107 publications

References 20 publications

Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins

Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins

C‐MYC and IGF‐II mRNA‐binding protein (CRD‐BP/IMP‐1) in benign and malignant mesenchymal tumors

HMGA2 Regulates Transcription of the Imp2 Gene via an Intronic Regulatory Element in Cooperation with Nuclear Factor-κB

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