Developmental Regulation of Elastin Synthesis

Davidson, Jeffrey M.; Shibahara, Shigeki; Smith, Kitty P.; Crystal, Ronald G.

doi:10.3109/03008208109152377

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…These sections were first incubated for 30 min at room temperature with rabbit antibody against sheep elastin at a dilution of 1:40 with phosphate-buffered saline (PBS), and washed three times with PBS. This antibody has been previously shown to be specific for elastin according to a number of criteria, which have been given in detail elsewhere (Shibahara et al, 1981;Davidson et al, 1981). The sections were then incubated for 30 min with peroxidase-labeled anti-rabbit-IgG goat IgG (Cappel Laboratories) a t 1:20 dilution, and washed three times with PBS.…”

Section: Light Microscopic Immunoperoxidase Methodsmentioning

confidence: 99%

The development of alveolar septa in fetal sheep lung. An ultrastructural and immunohistochemical study

1983

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The morphogenesis of pulmonary alveolar septa in the sheep was studied by light microscopy, transmission electron microscopy and light microscopic immunohistochemistry for the detection of elastin. The primordia of alveolar septa developed in the glandular stage in areas subjacent to the epithelium, and formed alveolar septa by protruding into the glandular lumina. In their earliest stage, the primordia consisted of groups of fibroblasts, which were associated with elastic fibers and unit collagen fibrils and were surrounded by epithelial basement membrane and by more immature fibroblasts. The fibroblasts in the primordia subsequently became myofibroblasts or smooth muscle cells. In the alveolar zone of the glands, elastic fibers were exclusively found in the primordia of alveolar septa in early developing lung. In early developing lung, wavy, thickened epithelial basement membranes were found in the regions of the glands, which eventually underwent considerable expansion of their surface areas, especially in the primordia of alveolar septa and the bifurcations in the alveolar zones. Areas of fusion of the basement membranes of capillary endothelial cells and epithelial cells in the alveolar zone were found after the formation of the primordia of alveolar septa was accomplished. These areas of fusion were not found in the primordia themselves, but in regions between the primordia. Epithelial cell flattening and differentiation occurred after the formation of the primordia of alveolar septa, and flattening was first observed in the areas of the primordia and the bifurcations of the alveolar zones.

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Section: Light Microscopic Immunoperoxidase Methodsmentioning

confidence: 99%

The development of alveolar septa in fetal sheep lung. An ultrastructural and immunohistochemical study

1983

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“…As noted, skin also contains a small mount of elastin but which is very thermally stable [151], for example elastin can survive boiling for several hours with no apparent change, and does not need attention here.…”

Section: Skin Biothermomechanicsmentioning

confidence: 98%

Biothermomechanical behavior of skin tissue

Seffen

2008

Acta Mech. Sin.

132

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Advances in laser, microwave and similar technologies have led to recent developments of thermal treatments involving skin tissue. The effectiveness of these treatments is governed by the coupled thermal, mechanical, biological and neural responses of the affected tissue: a favorable interaction results in a procedure with relatively little pain and no lasting side effects. Currently, even though each behavioral facet is to a certain extent established and understood, none exists to date in the interdisciplinary area. A highly interdisciplinary approach is required for studying the biothermomechanical behavior of skin, involving bioheat transfer, biomechanics and physiology. A comprehensive literature review pertinent to the subject is presented in this paper, covering four subject areas: (a) skin structure, (b) skin bioheat transfer and thermal damage, (c) skin biomechanics, and (d) skin biothermomechanics. The major problems, issues, and topics for further studies are also outlined. This review finds that significant advances in each of these aspects have been achieved in recent years. Although focus is placed upon the biothermomechanical behavior of skin tissue, the fundamental concepts and methodologies reviewed in this paper may also be applicable for studying other soft tissues.

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“…In old animals, independent of the parameters used, elastin was significantly (2/?<0.001) reduced compared with that in younger ages. Incomplete knowledge of the mechanisms involved in the regulation of elastin synthesis 38 hinders the interpretation of these phenomena. The age-related modifications of the elastic component might be related to altered ratios among extracellular matrix proteins, such as collagen or glycosylated molecules 41 ; to intrinsic rearrangements of elastic fiber constituents as glycosaminogrycans, 42 lysyl oxidase, 43 and vitronectin 44 ; and finally, to the dramatic reduction of elastin mRNA expression.…”

Section: Old Rats Note That Elastin (E) Is Abundantly Deposited In Tmentioning

confidence: 99%

Role of the extracellular matrix in age-related modifications of the rat aorta. Ultrastructural, morphometric, and enzymatic evaluations.

Fornieri

Quaglino

Mori

1992

Arterioscler Thromb

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Connective tissues such as blood vessels are known to be greatly affected by age because of impaired functional properties and increased susceptibility to diseases. With the aim of providing further information on the role of the extracellular matrix in age-related modifications, we investigated the aorta in the rat model from birth to senescence by means of morphological and morphometric observations and by evaluation of lysyl oxidase activity. Results focused on the dramatic vascular rearrangements due to progressive fibrosis of the extracellular matrix and on prominent elastin modifications. The presence of lysyl oxidase activity, even in the oldest animals, might be at least partly responsible for the increased stiffness of the aging extracellular matrix. The striking age-related remodeling of the aortic architecture and the alterations of the interactions between cellular and extracellular compartments might greatly influence the functional properties of the arterial wall in senescence, at least contributing to the consequences of some apparently age-related vascular disorders. ( integrated system composed of collagen fibrils, elastic lamellae, proteoglycans, minor collagens, and structural glycoproteins that, together with cells, guarantees the major properties of the vascular compartment, i.e., the diffusion at proper rates of nutrients and other metabolic factors through optimal blood flow. -4Apart from calcifications, fibrosis, and atheromatous lesions, which are frequently observed in the vessel wall of several species 5 " 9 and which are at least partly responsible for the failure of the cardiovascular system with age, 7 little is known about the pathogenesis of the age-related modifications of the vessel wall, which may affect vascular properties such as elasticity and resilience and which may be of paramount importance in understanding specific pathological conditions. The present work was undertaken with the aim of focusing attention on the age-related modifications of the aortic arch in rats from birth to senescence. Qualitative and quantitative morphological data concerning the structure and distribution of the cellular and extracellular components were correlated with the activity of lysyl oxidase, the enzyme that catalyzes the formation of collagen and elastin cross-links and therefore contributes to the stabilization of these two polymers. Supported by Fondi (40%) and Ministero Universita e Ricerca Scientifica e Technologica (60%).Address for correspondence: Prof. C. Fornieri, 1st. Patologia Generale, Via Campi 287, 41100 Modena, Italy.Received April 21, 1992; accepted May 13, 1992. In the light of our results, we suggest that the interactions of the cellular and the extracellular compartments and the ratio between collagen and elastin play key roles in conditioning the morphological-functional properties of the arterial wall and, therefore, explain at least some of the consequences (although not the pathogenesis) of several pathological processes affecting the vascular compartment with inc...

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Developmental Regulation of Elastin Synthesis

Cited by 16 publications

References 18 publications

The development of alveolar septa in fetal sheep lung. An ultrastructural and immunohistochemical study

The development of alveolar septa in fetal sheep lung. An ultrastructural and immunohistochemical study

Biothermomechanical behavior of skin tissue

Role of the extracellular matrix in age-related modifications of the rat aorta. Ultrastructural, morphometric, and enzymatic evaluations.

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