Developmental role of fatty acid-binding proteins in mouse brain

Sellner, Peggy A.; Chu, Wenjiang; Glatz, Jan F. C.; Berman, Nancy E.J.

doi:10.1016/0165-3806(95)00099-y

Cited by 46 publications

(38 citation statements)

References 44 publications

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“…Comparisons of 2-DE maps showed two distinct spots highly expressed in the CSF of deceased patients, both of which being definitely identified by various MS techniques as two forms of FABP. The FABP family encompasses at least eight different proteins (14) functionally involved in intracellular transport systems, oxidation of fatty acids, and membrane lipids trafficking (15,16). Brain-FABP (B-FABP) is localized exclusively in neuronal and glial cells (17), whereas H-FABP has a more widespread distribution including, in addition to brain, myocardial muscle, endothelial cells, lung, and kidney (18).…”

Section: Discussionmentioning

confidence: 99%

Fatty Acid Binding Protein as a Serum Marker for the Early Diagnosis of Stroke

Zimmermann‐Ivol

Burkhard

Floch-Rohr

et al. 2004

Molecular & Cellular Proteomics

134

103

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No biological marker is currently available for the routine diagnosis of stroke. The aim of this pilot study was to determine whether heart-fatty acid binding protein (H-FABP) could be used as a valid diagnostic biomarker for stroke, as compared with neuron-specific enolase (NSE) and S100B proteins. Using two-dimensional gel electrophoresis separation of cerebrospinal fluid proteins and mass spectrometry techniques, FABP was found elevated in the cerebrospinal fluid of deceased patients, used as a model of massive brain damage. Because H-FABP, a FABP form present in many organs, is also localized in the brain, an enzyme-linked immunosorbant assay was developed to detect H-FABP in stroke versus control plasma samples. However, H-FABP being also a marker of acute myocardial infarction (AMI), troponin-I and creatine kinase-MB levels were assayed at the same time in order to exclude any concomitant heart damage. NSE and S100B levels were assayed simultaneously. These assays were assessed in serial plasma samples from 22 control patients with no AMI or stroke, 20 patients with AMI but no stroke, and 22 patients with an acute stroke but no AMI. Twenty-two out of the 22 control patients and 15 out of the 22 stroke patients were correctly classified, figures much better than those obtained with NSE or S100B, in the same study's population. H-FABP appears to be a valid serum biomarker for the early diagnosis of stroke. Further studies on large cohorts of patients are warranted.

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Section: Discussionmentioning

confidence: 99%

Fatty Acid Binding Protein as a Serum Marker for the Early Diagnosis of Stroke

Zimmermann‐Ivol

Burkhard

Floch-Rohr

et al. 2004

Molecular & Cellular Proteomics

134

103

View full text Add to dashboard Cite

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“…Although several studies have described the occurrence of B-FABP and H-FABP in specific cells in the brain (31,32 ), because of a lack of quantitative data the total tissue concentrations of these proteins were unknown, as was their possible clinical utility. We therefore developed a B-FABP ELISA that measures the concentrations of both B-FABP and H-FABP in the brain and investigated whether brain injury in MTBI and in ECT can be detected by the release of these proteins into the blood serum.…”

Section: Discussionmentioning

confidence: 99%

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

Pelsers¹,

Hanhoff

Voort³

et al. 2004

Clinical Chemistry

109

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“…Membrane specialization involves not only the synthesis and transport of proteins, but also the synthesis and concentration of lipids (Sellner et al, 1995). Cluster 4 contains Elovl2 (elongation of very long chain fatty acids-like 2) and Dhcr7 (7-dehydrocholesterol reductase), while Elovl4 and Plaa (Phospholipase A2, activating protein) are found in cluster 7.…”

Section: Plasma Membrane Specializationmentioning

confidence: 99%

Microarray analysis of the developing cortex

et al. 2006

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Abnormal development of the prefrontal cortex (PFC) is associated with a number of neuropsychiatric disorders that have an onset in childhood or adolescence. Although the basic laminar structure of the PFC is established in utero, extensive remodeling continues into adolescence. To map the overall pattern of changes in cortical gene transcripts during postnatal development, we made serial measurements of mRNA levels in mouse PFC using oligonucleotide microarrays. We observed changes in mRNA transcripts consistent with known postnatal morphological and biochemical events. Overall, most transcripts that changed significantly showed a progressive decrease in abundance after birth, with the majority of change between postnatal weeks 2 and 4. Genes with cell proliferative, cytoskeletal, extracellular matrix, plasma membrane lipid/transport, protein folding, and regulatory functions had decreases in mRNA levels. Quantitative PCR verified the microarray results for six selected genes: DNA methyltransferase 3A (Dnmt3a), procollagen, type III, alpha 1 (Col3a1), solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1), MARCKS-like 1 (Marcksl1), nidogen 1 (Nid1) and 3-hydroxybutyrate dehydrogenase (heart, mitochondrial) (Bdh).

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Developmental role of fatty acid-binding proteins in mouse brain

Cited by 46 publications

References 44 publications

Fatty Acid Binding Protein as a Serum Marker for the Early Diagnosis of Stroke

Fatty Acid Binding Protein as a Serum Marker for the Early Diagnosis of Stroke

Brain- and Heart-Type Fatty Acid-Binding Proteins in the Brain: Tissue Distribution and Clinical Utility

Microarray analysis of the developing cortex

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