Developmental role of the tomato Mediator complex subunit <scp>MED</scp>18 in pollen ontogeny

Pérez-Martín, Fernando; Yuste‐Lisbona, Fernando J.; Pineda, Benito; García‐Sogo, Begoña; Olmo, Iván del; Alché, Juan de Dios; Egea, Isabel; Flores, Francisco B.; Piñeiro, Manuel; Jarillo, José A.; Angosto, Trinidad; Capel, Juan; Moreno, Vicente; Lozano, Rafael

doi:10.1111/tpj.14031

Cited by 23 publications

(19 citation statements)

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“…9f). In a recent study we characterized a tomato mutant ( pollen deficient1 , pod1 ), which also displayed a significant reduction of pollen viability and yielded parthenocarpic fruits [75]. We also reported that the loss-of-function of MEDIATOR COMPLEX SUBUNIT 18 ( POD1 / SlMED18 ) is responsible for the alterations in reproductive development observed in pod1 .…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits

et al. 2019

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Background Tomato mutants altered in leaf morphology are usually identified in the greenhouse, which demands considerable time and space and can only be performed in adequate periods. For a faster but equally reliable scrutiny method we addressed the screening in vitro of 971 T-DNA lines. Leaf development was evaluated in vitro in seedlings and shoot-derived axenic plants. New mutants were characterized in the greenhouse to establish the relationship between in vitro and in vivo leaf morphology, and to shed light on possible links between leaf development and agronomic traits, a promising field in which much remains to be discovered. Results Following the screening in vitro of tomato T-DNA lines, putative mutants altered in leaf morphology were evaluated in the greenhouse. The comparison of results in both conditions indicated a general phenotypic correspondence, showing that in vitro culture is a reliable system for finding mutants altered in leaf development. Apart from providing homogeneous conditions, the main advantage of screening in vitro lies in the enormous time and space saving. Studies on the association between phenotype and nptII gene expression showed co-segregation in two lines ( P > 99%). The use of an enhancer trap also allowed identifying gain-of-function mutants through reporter expression analysis. These studies suggested that genes altered in three other mutants were T-DNA tagged. New mutants putatively altered in brassinosteroid synthesis or perception, mutations determining multiple pleiotropic effects, lines affected in organ curvature, and the first tomato mutant with helical growth were discovered. Results also revealed new possible links between leaf development and agronomic traits, such as axillary branching, flower abscission, fruit development and fruit cracking. Furthermore, we found that the gene tagged in mutant 2635-MM encodes a Sterol 3-beta-glucosyltransferase. Expression analysis suggested that abnormal leaf development might be due to the lack-off-function of this gene. Conclusion In vitro culture is a quick, efficient and reliable tool for identifying tomato mutants altered in leaf morphology. The characterization of new mutants in vivo revealed new links between leaf development and some agronomic traits. Moreover, the possible implication of a gene encoding a Sterol 3-beta-glucosyltransferase in tomato leaf development is reported. Electronic supplementary material The online version of this article (10.1186/s12870-019-1735-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits

et al. 2019

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“…To silence the VIPP1 gene, an interference RNA (RNAi) construct was generated as described in Perez-Martin et al (2018) , with some modifications. A 326-bp fragment of Solyc11g008990 cDNA was amplified using the VIPP1-RNAi_F/R primers and cloned in sense and antisense orientations into the pKannibal vector, as described in Wesley et al (2001) .…”

Section: Methodsmentioning

confidence: 99%

The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane

et al. 2020

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Since membranes play essential roles in all living beings, all cells have developed mechanisms for efficient and fast repair of membrane damage. In Escherichia coli , the Phage shock stress A (PspA) protein is involved in the maintenance of the integrity of its inner membrane in response to the damage produced by exposure to stress conditions. A role in thylakoid membrane maintenance and reorganization has been proposed for Vesicle Inducing Protein in Plastid 1 (VIPP1), the putative PspA ortholog in Arabidopsis thaliana . While some membranes of plant cells have been extensively studied, the biosynthesis and maintenance of chloroplast thylakoid membrane remains poorly known. Here, we report the cloning and functional characterization of the tomato ( Solanum lycopersicum L.) ortholog of Escherichia coli PspA and Arabidopsis thaliana VIPP1 , which we dubbed SlVIPP1 . Our genetic and molecular characterization of slvipp1 , an insertional mutant, allowed us to conclude that the tomato SlVIPP1 gene is needed for development, as Arabidopsis VIPP1 , but not Escherichia coli PspA . Homozygous slvipp1 tomato plants are albino and exhibit early lethality and highly aberrant chloroplast development with almost complete absence of thylakoids. The phenotype of tomato RNAi lines and that of additional slvipp1 alleles generated by CRISPR/Cas9 gene editing technology confirmed that the morphological and histological aberrations shown by slvipp1 homozygotes are caused by VIPP1 lack of function. We also found that tomato SlVIPP1 overexpression does not cause any visible effect on plant morphology and viability. Our work with slvipp1 plants evidences that SlVIPP1 is an essential gene required for tomato survival, since its function is crucial for the proper formation and/or maintenance of thylakoid membranes.

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“…Previous elegant studies have demonstrated that the temporal action of PCD in tapetal cells is critical for guarding the development of pollens and plant fertility (Chen & Liu, ; Tang et al , ). Although the timing of tapetal cell death initiation varies in different plant species, delayed or premature occurrence of PCD in tapetal cells in Arabidopsis, tomato ( Solanum lycopersicum ) and rice can cause strong defects in pollen development and thereby result in male sterility (Kawanabe et al , ; Ji et al , ; Pérez‐Martín et al , ).…”

Section: The Roles Of Autophagy In Key Processes Of Plant Reproductionmentioning

confidence: 99%

Boosting autophagy in sexual reproduction: a plant perspective

Mei

et al. 2020

New Phytologist

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Summary The key process of sexual reproduction is the successful fusion of the sperm and egg cell. Distinct from dynamic and flagellated animal sperm cells, higher flowering plant sperm cells are immotile. Therefore, plants have evolved a novel reproductive system to achieve fertilization and generate progenies. Plant sexual reproduction consists of multiple steps, mainly including gametophyte development, pollen–pistil recognition, pollen germination, double fertilization and postfertilization. During reproduction, active production, consumption and recycling of cellular components and energy are critically required to achieve fertilization. However, the underlying machinery of cellular degradation and turnover remains largely unexplored. Autophagy, the major catabolic pathway in eukaryotic cells, participates in regulating multiple aspects of plant activities, including abiotic and biotic stress resistance, pathogen response, senescence, nutrient remobilization and plant development. Nevertheless, a key unanswered question is how autophagy regulates plant fertilization and reproduction. Here, we focus on comparing and contrasting autophagy in several key reproductive processes of plant and animal systems to feature important distinctions and highlight future research directions of autophagy in angiosperm reproduction. We further discuss the potential crosstalk between autophagy and programmed cell death, which are often considered as two disconnected events in plant sexual reproduction.

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Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny

Cited by 23 publications

References 71 publications

Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits

Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits

The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane

Boosting autophagy in sexual reproduction: a plant perspective

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