Developmental SEM observations on an extracellular matrix in embryogenic calli ofDrosera rotundifolia andZea mays

Šamaj, Jozef; Bobák, M.; Blehová, Alžbeta; Krištín, J.; Auxtová-Šamajová, Olga

doi:10.1007/bf01276934

Cited by 48 publications

(28 citation statements)

References 12 publications

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“…The role of ECMSN cannot be generalized but it is evident that it is formed on the surface of cultured in vitro tissues regardless of its morphogenetic competence. Therefore, we agree with the suggestion that has been made by several authors [26,30] that ECMSN formation could be a stress response of plant tissues that is triggered by specific culture conditions and that covering callus with extracellular material could provide protection against external factors.…”

Section: Discussioncontrasting

confidence: 30%

“…A similar heterogeneous ECMSN was reported in oilseed rape where osmiophilic granules were associated with fibers extending from the outer cell wall of embryogenic callus cells [29]. ECMSN was primarily linked to the acquisition of embryogenic competence [8,30] and in several species can serve as an early structural marker of somatic embryogenesis [29,31,32]. The presence of extracellular material also accompanied the induction of organogenesis [12,33].…”

Section: Discussionmentioning

confidence: 69%

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Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

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Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

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Section: Discussioncontrasting

confidence: 30%

Section: Discussionmentioning

confidence: 69%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

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“…1 Šamaj et al, 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al, 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations.…”

mentioning

confidence: 99%

Are extracellular matrix surface network components involved in signalling and protective function?

Popielarska-Konieczna

Kozieradzka-Kiszkurno²,

Swierczynska³

et al. 2008

Plant Signaling & Behavior

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Endosperm is an interesting model for in vitro experiments, because of its unique origin, development and ploidy level. Here we used Actinidia deliciosa endosperm-derived callus to investigate morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM and TEM analysis showed that ECM is a heterogenous layer which consists of amorphous, dark-staining material, osmiophilic granules and reticulated fibres outside the outer callus cell wall. This structure may serve as a structural marker of morphogenic competence in endosperm-derived callus, because of its presence on the surface of callus forming morphogenic domains and its disappearance during organ growth. Based on immunolabelling, histochemistry, solvent and enzyme treatments, we suggest that pectins and lipids are components of the ECM layer. These results might indicate protective, water retention and/or cell communication functions for this ECM layer.In several plants cultured in vitro, SEM analysis revealed that induction of morphogenesis is linked to the appearance of a fibrillar network referred to as the extracellular matrix surface network (ECMSN). 1 Šamaj et al., 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al., 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations. There is not a large body of data confirming the occurrence of ECM during in vitro organogenesis. 5,6 Most data concern the formation, structure and chemical composition and function of ECM in somatic and androgenic embryogenesis (reviewed in refs. 6-10). In previous histological and SEM analysis of morphogenic endosperm-derived callus of A. deliciosa, we described the presence of a membranous layer covering the callus surface, which we termed extracellular matrix (ECM). 11 The present experiments employed SEM, TEM and histochemical analysis to study the structure and composition of material coating the callus surface.SEM revealed the presence of heterogeneous material covering the callus surface. A smooth membranous layer coated some parts of the callus surface (Fig. 1A), while other regions were covered by fibrillar structures and granules of mucilage-like secretion forming a network at site of cell-cell adhesion. Similar structures were observed during in vitro induction of somatic embryogenesis, androgenesis and organogenesis in different species (reviewed in refs. 7-13).TEM showed a layer composed of amorphous material with fibrillar and spherical components covering morphogenic cell clusters. The cell wall of non-embryogenic and senescent cells was also covered with ECM, but it differed in appearance: the layer was granular, with amo...

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“…The staining of this material with AzurI I/methylene blue suggested the presence of a mucilage/polysaccharide component (Namasivayam et al, 2006). A similar substance at the surface of the pre-embryogenic tissues was present in Coffea arabica (Sondahl et al, 1979), Cichorium (Chapman et al, 2000a(Chapman et al, , 2000bDubois et al 1991Dubois et al , 1992, Camellia japonica (Pedroso & Pais, 1992, 1995, Drosera (Bobák et al, 2003;Šamaj et al, 1995), Zea mays (Šamaj et al, 1995), Papaver (Ovečka et al, 1997;Šamaj et al, 1994), Pinus (Jasik et al, 1995), Citrus (Chapman et al, 2000a) and coconut (Verdeil et al, 2001). The detected material was present only up to the globular stage of embryo development.…”

Section: Changes In Apoplast As a Markers For Sementioning

confidence: 97%

Cellular Markers for Somatic Embryogenesis

Kurczyńska¹,

Potocka²,

Dobrowolska³

et al. 2012

Embryogenesis

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Developmental SEM observations on an extracellular matrix in embryogenic calli ofDrosera rotundifolia andZea mays

Cited by 48 publications

References 12 publications

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Are extracellular matrix surface network components involved in signalling and protective function?

Cellular Markers for Somatic Embryogenesis

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