Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Coveñas, Rafael; González-Fuentes, J.; Infante, Eloy Rivas; Lagartos-Donate, M.J.; Mangas, A.; Geffard, M.; Arroyo-Jiménez, M.M.; Cebada‐Sánchez, Sandra; Insausti, Ricardo; Marcos, Pilar

doi:10.1016/j.aanat.2015.06.001

Cited by 5 publications

(19 citation statements)

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“…Thick and smooth immunoreactive axons containing thiamine (vitamin B1), riboflavin (vitamin B2), pyridoxal (vitamin B6) or folic acid (vitamin B9) have been observed in the mesencephalon, thalamus, anterior commissure and/or cerebral cortex of the monkey (Macaca fasci cularis) [1][2][3][4][5], whereas cell bodies containing pyridoxal or vitamin C have been respectively visualized in the www.fhc.viamedica.pl hypothalamus and in the somatosensorial cortex (precentral gyrus) of the same species [4,5]. Moreover, the distribution of immunoreactive structures containing vitamin C has been recently reported in the brainstem of children [8] and direct visualization of retinoic acid, in perikarya located in the rat hypothalamus, was also reported [6]. Most of vitamins studied using IHC techniques belong to the B series (B1, B2, B6 and B9) [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 96%

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Mangas

Yajeya

González³

et al. 2017

Folia Histochem Cytobiol.

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Introduction. The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain. Material and methods. According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10 -8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain. Results. Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2-3 long (50-100 µm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected. Conclusions. The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.

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Section: Introductionmentioning

confidence: 96%

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Mangas

Yajeya

González³

et al. 2017

Folia Histochem Cytobiol.

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“…), whereas in a immunohistochemical study carried out in children, VitC was observed in several brainstem regions (Covenas et al. ). Our results are partially in agreement with these reports, although the distribution of SVCT2 described in the present study does not totally match the pattern described for VitC in children's brainstem.…”

Section: Discussionmentioning

confidence: 97%

“…; Covenas et al. ). VitC is involved in numerous functions, including its role as a co‐enzyme in noradrenalin synthesis and an agent in glutamate‐ and dopamine‐mediated transmission (Rebec & Pierce, ; Coveñas et al.…”

Section: Introductionmentioning

confidence: 98%

“…; Covenas et al. ). Moreover, several animal models have been developed to study the effects of VitC deprivation or SVCT2 mutation (for review, see Harrison et al.…”

Section: Introductionmentioning

confidence: 98%

“…; Covenas et al. ), liberation of gonadotropins (Figueroa‐Mendez & Rivas‐Arancibia, ) or neuronal maturation and myelin formation (Hansen et al. ).…”

Section: Introductionmentioning

confidence: 99%

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Vitamin transporters in mice brain with aging

et al. 2018

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Its high metabolic rate and high polyunsaturated fatty acid content make the brain very sensitive to oxidative damage. In the brain, neuronal metabolism occurs at a very high rate and generates considerable amounts of reactive oxygen species and free radicals, which accumulate inside neurons, leading to altered cellular homeostasis and integrity and eventually irreversible damage and cell death. A misbalance in redox metabolism and the subsequent neurodegeneration increase throughout the course of normal aging, leading to several age-related changes in learning and memory as well as motor functions. The neuroprotective function of antioxidants is crucial to maintain good brain homeostasis and adequate neuronal functions. Vitamins E and C are two important antioxidants that are taken up by brain cells via the specific carriers αTTP and SVCT2, respectively. The aim of this study was to use immunohistochemistry to determine the distribution pattern of these vitamin transporters in the brain in a mouse model that shows fewer signs of brain aging and a higher resistance to oxidative damage. Both carriers were distributed widely throughout the entire brain in a pattern that remained similar in 4-, 12-, 18- and 24-month-old mice. In general, αTTP and SVCT2 were located in the same regions, but they seemed to have complementary distribution patterns. Double-labeled cell bodies were detected only in the inferior colliculus, entorhinal cortex, dorsal subiculum, and several cortical areas. In addition, the presence of αTTP and SVCT2 in neurons was analyzed using double immunohistochemistry for NeuN and the results showed that αTTP but not SVCT2 was present in Bergmann's glia. The presence of these transporters in brain regions implicated in learning, memory and motor control provides an anatomical basis that may explain the higher resistance of this animal model to brain oxidative stress, which is associated with better motor performance and learning abilities in old age.

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Neurobiology of vitamin C: Expanding the focus from antioxidant to endogenous neuromodulator

Ballaz

Rebec

2019

Pharmacological Research

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Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Cited by 5 publications

References 43 publications

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody

Vitamin transporters in mice brain with aging

Neurobiology of vitamin C: Expanding the focus from antioxidant to endogenous neuromodulator

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