Developmental toxicity of glyphosate on embryo-larval zebrafish (Danio rerio)

Liu, Zhihui; Shangguan, Yingying; Zhu, Penglin; Sultan, Yousef; Feng, Yiyi; Li, Xiaoyü; Ma, Jing

doi:10.1016/j.ecoenv.2022.113493

Cited by 56 publications

(16 citation statements)

References 80 publications

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“…Studies have shown that when IGF-1 can be used as a marker of abortion [ 33 ]. It was also found that IGF-1 could be regulated to inhibit growth and development [ 34 ]. In conclusion, the above targets were strongly associated with the occurrence of embryotoxicity.…”

Section: Discussionmentioning

confidence: 99%

The Mechanism of Houttuynia cordata Embryotoxicity Was Explored in Combination with an Experimental Model and Network Pharmacology

Liu

Yang

Yang³

et al. 2023

Toxins

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Houttuynia cordata (H. cordata) is the most common herb as a food and traditional Chinese medicine. Currently, studies on its toxicity have mainly focused on hepatotoxicity. However, its potential embryotoxicity by long-term exposure is often overlooked. Objective: To investigate the effects of H. cordata on embryonic development and its toxicity mechanism by combining network pharmacology, molecular docking, and in vitro experimental methods. Methods: The effects of H. cordata on embryos were evaluated. Zebrafish embryos and embryoid bodies were administered to observe the effects of H. cordata on embryonic development. Based on network pharmacological analysis, it was found that the main active agents producing toxicity in H. cordata were oleanolic acid, lignan, and aristolactam AII. H. cordata can affect PI3K-Akt, MAPK, and Ras signaling pathways by regulating targets, such as AKT1, EGFR, CASP3, and IGF-1. RT-PCR and immunohistochemistry results showed that the expression of AKT1 and PI3K in the embryoid body was significantly reduced after drug administration (p < 0.05). Conclusions: The results of network pharmacology and in vitro experiments suggest that H. cordata may affect embryonic development by influencing the PI3K-Akt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

The Mechanism of Houttuynia cordata Embryotoxicity Was Explored in Combination with an Experimental Model and Network Pharmacology

Liu

Yang

Yang³

et al. 2023

Toxins

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“…The dead embryos were removed and counted daily. The hatching rate was recorded at 48 h and 72 h. The heartbeat rate for 30 s and the length of the larvae were also recorded using a stereoscopic microscope (Olympus SZ61, Tokyo, Japan) by an accessory software according to a previously described experimental method [ 21 ]…”

Section: Methodsmentioning

confidence: 99%

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Ding

Chen

Sultan

et al. 2022

Animals

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Previous studies have shown that ILs can induce toxicity in animals, plants, and cells. However, the effect of imidazolium-based ILs on the hypothalamus–pituitary–thyroid (HPT) axis of fish remains unknown. The present study aimed to evaluate the acute effect of [C8mim]Cl on the embryonic development and thyroid-controlled internal secretion system of zebrafish by determining the thyroid hormone level and the expression of HPT-related genes. The results obtained for embryonic developmental toxicity showed the survival rate, heart beats, and body length of fish had decreased 96 h after exposure to [C8mim]Cl, but the hatching rate had increased by the 48 h time point. The transcription levels of HTP-related genes showed that the genes dio3, tg, ttr, tsh, trhrα, trhrβ, trhr2, and tpo were up-regulated, while the expression levels of dio1, trh, tshr, and nis were significantly suppressed. Furthermore, we found that exposure to [C8mim]Cl induced an alteration in the levels of thyroid hormones that increased the T3 but decreased the T4 content. In conclusion, our study indicated that acute exposure to [C8mim]Cl altered the expression of HTP-related genes and disturbed the thyroid hormone level, suggesting that the ionic liquid [C8mim]Cl might pose an aquatic environmental threat to fish.

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“…The liver is a major site of vertebrate material metabolism, and the zebrafish liver contains enzymes capable of metabolizing multiple endogenous and exogenous compounds similar to the human liver. Because some drugs or chemicals can cause liver damage in zebrafish, including the occurrence of vacuolization in the liver (Duan et al, 2022;Hu et al, 2022;Huang, Liu, et al, 2022;Wang, Huang, Liu, et al, 2022), liver size changes and liver steatosis , apoptosis and necrotic Siqueira et al, 2022), liver inflammation, dysregulation of lipid metabolism and glycolysis in the liver (Li et al, 2022), and hepatic lipid peroxidation (Song et al, 2022). See Table 4 for the details.…”

Section: Hepatotoxicity In Zebrafishmentioning

confidence: 99%

Frontier of toxicology studies in zebrafish model

Shao,

Xiao,

Yang

et al. 2023

J of Applied Toxicology

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Based on the 87 original publications only from quartiles 1 and 2 of Journal Citation Report (JCR) collected by the major academic databases (Science Direct, Web of Science, PubMed, and Wiley) in 2022, the frontier of toxicology studies in zebrafish model is summarized. Herewith, a total of six aspects is covered such as developmental, neurological, cardiovascular, hepatic, reproductive, and immunizing toxicities. The tested samples involve chemicals, drugs, new environmental pollutants, nanomaterials, and its derivatives, along with those related mechanisms. This report may provide a frontier focus benefit to researchers engaging in a zebrafish model for environment, medicine, food, and other fields.

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Developmental toxicity of glyphosate on embryo-larval zebrafish (Danio rerio)

Cited by 56 publications

References 80 publications

The Mechanism of Houttuynia cordata Embryotoxicity Was Explored in Combination with an Experimental Model and Network Pharmacology

The Mechanism of Houttuynia cordata Embryotoxicity Was Explored in Combination with an Experimental Model and Network Pharmacology

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Frontier of toxicology studies in zebrafish model

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