Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application

Zhu, Bofeng; Zhang, Yu-Dang; Shen, Chunmei; Du, Weian; Liu, Wenjuan; Meng, Hao-Tian; Wang, Hongdan; Yang, Guang; Jin, Rui; Yang, Chunhua; Yan, Jiangwei; Bie, Xiaohua

doi:10.1002/elps.201400333

Cited by 50 publications

(18 citation statements)

References 26 publications

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“…In total, 57 Han samples and Control DNA 007 were typed in parallel with the GlobalFiler PCR Amplification Kit (Thermo Fisher Scientific) to compare the profiles across overlapping loci (the expanded CODIS 20 core loci) obtained by CE–STR and MPS–STR methods. The 58 Tibetan samples and Control DNA 9947A were genotyped with the Huaxia Platinum System (Thermo Fisher Scientific) and the AGCU 21 + 1 STR kit (AGCU) to obtain the CE profiles of all 31 autosomal STRs in the Precision ID GlobalFiler NGS STR Panel version 2. All the CE experiments were carried out according to the manufacturer's protocol, and every plate included Control DNA 007 as a positive control and a nuclease‐free water sample as a negative control sample.…”

Section: Methodsmentioning

confidence: 99%

Characterization of sequence variation at 30 autosomal STRs in Chinese Han and Tibetan populations

Wang

Liu

et al. 2020

Electrophoresis

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Massively parallel sequencing (MPS) technologies have the ability to reveal sequence variations within STR alleles as well as their nominal allele lengths, which have traditionally been detected by CE instruments. Recently, Thermo Fisher Scientific has updated the MPS-STR panel, named the Precision ID GlobalFiler next-generation sequencing (NGS) STR Panel version 2, with primers redesigned to add two pentanucleotide tandem repeat loci and profile interpretation supported by the Converge software. Using the Ion Chef System, the Ion S5XL System, and the Converge software, genetic variations were characterized within STR repeat and flanking regions of 30 autosomal STR markers in 115 unrelated individuals from two Chinese population groups (58 Tibetans and 57 Hans). Nineteen STRs demonstrated a relative increase in diversity with the variant sequence alleles compared with those of traditional nominal length alleles. In total, 390 alleles were identified by their sequences compared with 258 alleles that were identified by length. Of these 92 sequence variants found within the STR repeat regions, 40 variants were located in STR flanking regions. Additionally, the agreement of the results with CE data was evaluated, as was the ability of this new MPS panel to analyze case-type (11 samples) and artificially degraded samples (seven samples in triplicate). The results generated from this study illustrate that extensive sequence variation exists in commonly used STR markers in the selected population samples and indicate that this NGS STR panel has the potential to be used as an effective tool for human forensics.

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Section: Methodsmentioning

confidence: 99%

Characterization of sequence variation at 30 autosomal STRs in Chinese Han and Tibetan populations

Wang

Liu

et al. 2020

Electrophoresis

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“…Short tandem repeats (STRs) are being widely used in forensic DNA analysis, considering that they are diversely distributed in the human genome and present the high discrimination power among the individuals (Yao & Wang, ; Zhu et al, ). STR, as the common genetic markers in forensic DNA analysis, can provide a great powerful discrimination capability.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Population genetics of 24 Y‐STR loci in Chinese Han population from Jilin Province, Northeast China

Zheng

Lou

et al. 2019

Molec Gen & Gen Med

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BackgroundIt is meaningful to expand the available population information on forensic medicine and to investigate the genetic characteristics of Han population from Jilin Province, Northeast China.MethodsIn this study, we investigated the genetic characteristics of 24 Y‐chromosomal short tandem repeat (STR) loci in 1,088 unrelated Chinese Han male individuals from Jilin Province, using DNATyperTM Y24 amplification kit. Additionally, we performed the population comparison between the Jilin population and the other nine reported populations based on the Y‐STR genotyping haplotypes.ResultsA total of 1,067 different haplotypes were found from 1,088 unrelated individuals, of which 1,046 were unique and 21 were shared by two individuals. The gene diversity values of 22 loci ranged from 0.3870 (DYS391) to 0.9668 (DYS385ab). The random match probability was 0.0010 with the discrimination capacity of 0.9807. Population comparison showed that there were minor differences compared to Beijing Chinese Han, China Manchu, Gansu Chinese Han, and Jiangsu Chinese Han, but major differences with respect to the populations of Guangdong Chinese Han, Yunnan Chinese Han, China Hui, China Korean, and China Tibetan.ConclusionOur results showed that the 24 Y‐STR loci in Jilin Han population are valuable for forensic application and human genetics.

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“…The AGCU 21 + 1 System (AGCU ScienTech Incorporation) was specifically developed for forensic application in Chinese populations 20 . This system is a 22-locus, five-dye, multiplex that allows co-amplification and fluorescent detection of the 21 non-CODIS STRs (D10S1248, D10S1435, D11S4463, D12ATA63, D14S1434, D17S1301, D18S853, D19S433, D1GATA113, D1S1627, D1S1677, D20S482, D22S1045, D2S1776, D2S441, D3S4529, D4S2408, D5S2500, D6S1017, D6S474, and D9S1122) as well as Amelogenin for gender determination.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Phylogenetic Differentiation of Southwestern Chinese Han: a comprehensive and comparative analysis on 21 non-CODIS STRs

Wang

et al. 2017

Sci Rep

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Short tandem repeats (STRs), with high polymorphism and complex evolution information, play a significant role in genetic association studies like population genetics, molecular anthropology and human forensics. However, human genetic diversity has only been partially sampled and available for Southwest Chinese Han population, as well as the genetic architecture of this population remains uncharacterized. In this work, 368 unrelated Han individuals from Sichuan province were firstly genotyped with 21 non-CODIS autosomal STRs, and phylogenetic relationships along administrative (Han Chinese from different regions) and ethnic divisions (minority ethnic groups) were subsequently investigated. The CMP and CPE were 6.2796 × 10−20 and 0.9999999, respectively. Analysis of molecular variance (AMOVA), principal component analysis (PCA), multidimensional scaling plots (MDS) and phylogenetic analysis consistently demonstrated that the Southwest Han population had a close genetic relationship with the geographically close population (Hunan Han) and kept a distant genetic relationship with some ethnic groups, most prominently for Gansu Yugu and Fujian She. Furthermore, no significant genetic distinction between the Northern Han and Southern Han was observed. Aforementioned results suggested that these 21 STRs are highly polymorphic and informative, which are suitable for human identification and population genetics.

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Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application

Cited by 50 publications

References 26 publications

Characterization of sequence variation at 30 autosomal STRs in Chinese Han and Tibetan populations

Characterization of sequence variation at 30 autosomal STRs in Chinese Han and Tibetan populations

RETRACTED: Population genetics of 24 Y‐STR loci in Chinese Han population from Jilin Province, Northeast China

Genetic Diversity and Phylogenetic Differentiation of Southwestern Chinese Han: a comprehensive and comparative analysis on 21 non-CODIS STRs

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