Developmental validation of the Microreader™ RM-Y ID System: a new rapidly mutating Y-STR 17-plex system for forensic application

Zhou, Yuxiang; Song, Feng; Dai, Hao; Wang, Shuangshuang; Zhang, Ke; Wei, Xiaowen; Wang, Xindi; Luo, Haibo

doi:10.1007/s00414-021-02632-w

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…All the mutation events were one‐step mutations and were found at RM Y‐STRs except DYS439 [15, 16] as the previous studies suggested [8]. DYS627 was the locus with the most mutation events (9/29), which was consistent with Zhou [17], ignoring DYF399S1 and DYS612 that were not contained in the Microreader Group Y Direct ID System. Losing one repeat was observed more than gaining one in these 44 pairs at most of the RM Y‐STR, except DYS449 (losses/gains: 0/3) or DYF387S1 (losses/gains: 1/3).…”

Section: Resultssupporting

confidence: 80%

Developmental validation of the Microreader Group Y Direct ID System: A novel six‐dye typing system with 54 Y‐chromosomal loci for forensic application

Song

Jiang

Lyu³

et al. 2022

Electrophoresis

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Y-chromosome, as a gender-determined biological marker, is inherited only between fathers and sons. The Y-chromosome short tandem repeats (Y-STRs) play an essential role in paternity lineage tracing as well as sexual assault cases. The Microreader Group Y Direct ID System as a six-dye multiplex amplification kit, including 53 Y-STR and one Y-Indel locus, would improve performance and aid in obtaining more information through a greater number of loci with high polymorphism. In the present study, to verify the accuracy and efficiency of the kit, developmental validation was conducted by investigating sensitivity, species specificity, PCR inhibition, male-male and male-female mixtures, and reproducibility. The kit was tested using 311 male samples from Han and Qiang populations in Sichuan Province. The results showed that this kit had fairly high power for forensic discrimination (Han: haplotype diversity [HD] = 1, Qiang: HD = 0.999944). Additionally, 44 confirmed father-son pairs were also genotyped, among which 69 distinct haplotypes could be obtained. These father-son pairs cannot be distinguished by commonly used Y-STR panels, indicating that adding these extra Y-STRs to a single panel can achieve better discrimination performance. Collectively, the Microreader Group Y Direct ID System is robust and informative for forensic applications.

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Section: Resultssupporting

confidence: 80%

Developmental validation of the Microreader Group Y Direct ID System: A novel six‐dye typing system with 54 Y‐chromosomal loci for forensic application

Song

Jiang

Lyu³

et al. 2022

Electrophoresis

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“…For the remaining loci (DYS446, DYS458, DYS464, DYS516, DYS534, DYS630, DYS713, and Y-GATA-A10), Feng et al (2020) found that DYS446, DYS458, DYS630, and Y-GATA-A10 displayed relatively high GD values (>0.5) in four minority groups from China. Zhou et al (2021) discovered that mutation rates of DYS464 and DYS713 loci in the Sichuan Han population were 4 × 10 –3 and 2.4 × 10 –2 , respectively. Fan et al (2021b) reported that mutation rates of DYS516 and DYS534 loci in the coastal southeastern Han populations were 3.89 × 10 –3 and 1.07 × 10 –2 , respectively.…”

Section: Resultsmentioning

confidence: 99%

“… Zhang et al (2017) designed a multiplex panel of 13 RM Y-STRs and evaluated their mutation rates in Chinese Han populations, showing that nearly 20% of father–son pairs could be differentiated by these loci. Zhou et al (2021) reported a validation study of a multiplex amplification system including 17 RM Y-STRs with high DC in Chinese male individuals. These RM Y-STR loci could expand the power of male identification if commercially available as a kit.…”

Section: Introductionmentioning

confidence: 99%

Developmental Validation of a Rapidly Mutating Y-STR Panel Labeled by Six Fluoresceins for Forensic Research

et al. 2022

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The male-specific region of the human Y chromosome is a useful genetic marker for genealogical searching, male inheritance testing, and male DNA mixture deconvolution in forensic studies. However, the Y chromosomal short tandem repeats (Y-STRs) are difficult to distinguish among related males due to their low/medium mutation rate. In contrast, rapidly mutating (RM) Y-STRs exhibit unusually high mutation rates and possess great potential for differentiating male lineages. In this study, we developed a novel Y-STRs multiplex amplification assay of 32 RM Y-STRs by fragment analysis using six dye-labeled technologies (FAM, HEX, TAMRA, ROX, VIG, and SIZ). The development and the validation of the kit were carried out in accordance with the Scientific Working Group guidelines on DNA Analysis Methods. Identical allelic profiles of the 32 RM Y-STRs using a DNA 9948 sample as the positive control could be observed at different concentrations of PCR reagents. Further, the RM Y-STRs did not show cross-reactions with other common animal species, and the developed assay could tolerate interferences from common PCR inhibitors and mixed DNA samples. More importantly, the kit showed relatively high sensitivity and could detect trace DNA samples. Genetic distributions of 32 RM Y-STRs in the Guizhou Han population revealed that these RM Y-STRs showed relatively high genetic diversities. In conclusion, the RM Y-STR assay developed here showed good species specificity, high sensitivity, tolerance to inhibitors, and sample compatibility, which can be viewed as a highly efficient tool with high discrimination capacity for forensic male differentiation.

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“…A number of Y-STR typing systems have been described along with validation studies, such as a 36plex [ 453 ], a 41plex [ 454 ], a 29plex [ 455 ], a 17plex [ 456 ], a 24plex [ 457 ], the Microreader 40Y ID System [ 458 ], the 24 Y-STRs in the AGCU Y SUPP STR kit [ 459 ], the DNATyper Y26 PCR amplification kit [ 460 ], a multiplex with 12 multicopy Y-STR loci [ 461 ], the Yfiler Platinum PCR Amplification Kit [ 462 ], a 45plex [ 463 ], the Microreader 29Y Prime ID system [ 464 ], an assay with 30 slow and moderate mutation Y-STR markers [ 465 ], the 17plex Microreader RM-Y ID System [ 466 ], and a 26plex for rapidly mutating Y-STRs [ 467 ]. A machine learning program predicted Y haplogroups using two Y-STR multiplexes with 32 Y-STRs [ 468 ].…”

Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Developmental validation of the Microreader™ RM-Y ID System: a new rapidly mutating Y-STR 17-plex system for forensic application

Cited by 7 publications

References 33 publications

Developmental validation of the Microreader Group Y Direct ID System: A novel six‐dye typing system with 54 Y‐chromosomal loci for forensic application

Developmental validation of the Microreader Group Y Direct ID System: A novel six‐dye typing system with 54 Y‐chromosomal loci for forensic application

Developmental Validation of a Rapidly Mutating Y-STR Panel Labeled by Six Fluoresceins for Forensic Research

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

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