Developmental validation of the Yfiler® Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications

Gopinath, Siddhita; Zhong, Chang; Nguyen, Vivian T.; Ge, Jianye; Lagacé, Robert; Short, Marc L.; Mulero, Julio J.

doi:10.1016/j.fsigen.2016.07.006

Cited by 109 publications

(71 citation statements)

References 20 publications

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“…Starting in 1992 with the publication of the first polymorphic STR discovered on the non-recombining part of the Y-chromosome (Roewer et al 1992), and its immediate application to forensic casework (Roewer and Epplen 1992), more and more Y-STR markers were subsequently developed for forensic Y-STR haplotyping (Gopinath et al 2016; Hall and Ballantyne 2003; Hanson and Ballantyne 2004, 2007; Kayser et al 1997; Krenke et al 2005; Lim et al 2007; Mulero et al 2006; Rodig et al 2008; Thompson et al 2013; Vermeulen et al 2009). Up to 27 markers are currently included in commercial Y-STR kits [Yfiler Plus, Thermo Fisher Scientific (Gopinath et al 2016)] (Table 1).…”

Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

“…Up to 27 markers are currently included in commercial Y-STR kits [Yfiler Plus, Thermo Fisher Scientific (Gopinath et al 2016)] (Table 1). Due to the achieved high haplotype diversity, these tools allow for the characterization of a paternal lineage with high, albeit not maximal, degree of certainty, especially when the tested sample donor comes from an outbred population (Purps et al 2014; Vermeulen et al 2009).…”

Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

“…Moreover, these commercial Y-STR kits allow the detection and characterization of DNA from males in mixed stains with high excess of DNA from females, also in cases with very low quantities of DNA from the minor male contributor as typical in material from sexual assault (Purps et al 2015). Recommendations on forensic analysis of Y-STRs have been established by the DNA Commission of the International Society of Forensic Genetics (Gill et al 2001; Gusmao et al 2006), and the Y-STR kits have forensically been validated (Gopinath et al 2016; Krenke et al 2005; Mulero et al 2006; Thompson et al 2013). This allows forensic practitioners not only to exclude male suspects from being involved in a crime via non-matching Y-STR haplotypes, but also to identify the paternal lineage that a trace donor belongs to via matching Y-STR haplotypes (Roewer 2009).…”

Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

See 2 more Smart Citations

Forensic use of Y-chromosome DNA: a general overview

2017

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The male-specific part of the human Y chromosome is widely used in forensic DNA analysis, particularly in cases where standard autosomal DNA profiling is not informative. A Y-chromosomal gene fragment is applied for inferring the biological sex of a crime scene trace donor. Haplotypes composed of Y-chromosomal short tandem repeat polymorphisms (Y-STRs) are used to characterise paternal lineages of unknown male trace donors, especially suitable when males and females have contributed to the same trace, such as in sexual assault cases. Y-STR haplotyping applied in crime scene investigation can (i) exclude male suspects from involvement in crime, (ii) identify the paternal lineage of male perpetrators, (iii) highlight multiple male contributors to a trace, and (iv) provide investigative leads for finding unknown male perpetrators. Y-STR haplotype analysis is employed in paternity disputes of male offspring and other types of paternal kinship testing, including historical cases, as well as in special cases of missing person and disaster victim identification involving men. Y-chromosome polymorphisms are applied for inferring the paternal bio-geographic ancestry of unknown trace donors or missing persons, in cases where autosomal DNA profiling is uninformative. In this overview, all different forensic applications of Y-chromosome DNA are described. To illustrate the necessity of forensic Y-chromosome analysis, the investigation of a prominent murder case is described, which initiated two changes in national forensic DNA legislation both covering Y-chromosome use, and was finally solved via an innovative Y-STR dragnet involving thousands of volunteers after 14 years. Finally, expectations for the future of forensic Y-chromosome DNA analysis are discussed.

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Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

Section: Y-strs For Paternal Lineage Identificationmentioning

confidence: 99%

See 1 more Smart Citation

Forensic use of Y-chromosome DNA: a general overview

2017

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“…Although, the analysis of Ychromosomal markers can provide complementary information in addition to an autosomal genetic profile [168], the most common application of Y-STRs is in cases of sexual assault, when the female component can greatly overshadow the male component, making autosomal STR profiling frequently difficult, unclear or impossible. Examination of Y-chromosome markers is available in a wide range of commercial kits [12, 169,170] which perform adequately for identifying male lineages.…”

Section: Brief History Of Uniparental Lineage Markersmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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“…Among the various markers in Y chromosome, short tandem repeats (STRs), are widely employed due to their hypervariability and hypermutability, thus it is available to perform multiplex amplifications at Y-STRs. So far, several commercial kits have been developed, such as PowerPlex Y (Promega, USA) [9], Yfiler™ Plus (Thermo Fisher Scientific, USA) [10], and PowerPlex ® Y23 [11]. The population data of Y-STRs now is being enriched to explore the genetic diversity of global male lineages [12–15].…”

Section: Introductionmentioning

confidence: 99%

Inner and inter population structure construction of Chinese Jiangsu Han population based on Y23 STR system

HuiPin¹,

Ba²,

Yang³

et al. 2017

PLoS ONE

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In this study, we analyzed the genetic polymorphisms of 23 Y-STR loci from PowerPlex® Y23 system in 916 unrelated healthy male individuals from Chinese Jiangsu Han, and observed 912 different haplotypes including 908 unique haplotypes and 4 duplicate haplotypes. The haplotype diversity reached 0.99999 and the discrimination capacity and match probability were 0.9956 and 0.0011, respectively. The gene diversity values ranged from 0.3942 at DYS438 to 0.9607 at DYS385a/b. Population differentiation within 10 Jiangsu Han subpopulations were evaluated by RST values and visualized in Neighbor-Joining trees and Multi-Dimensional Scaling plots as well as population relationships between the Jiangsu Han population and other 18 Eastern Asian populations. Such results indicated that the 23 Y-STR loci were highly polymorphic in Jiangsu Han population and played crucial roles in forensic application as well as population genetics. For the first time, we reported the genetic diversity of male lineages in Jiangsu Han population at a high-resolution level of 23 Y-STR set and consequently contributed to familial searching, offender tracking, and anthropology analysis of Jiangsu Han population.

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Developmental validation of the Yfiler® Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications

Cited by 109 publications

References 20 publications

Forensic use of Y-chromosome DNA: a general overview

Forensic use of Y-chromosome DNA: a general overview

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Inner and inter population structure construction of Chinese Jiangsu Han population based on Y23 STR system

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