Developmentally and stress‐induced small heat shock proteins in cork oak somatic embryos

Puigderrajols, Pere; Jofré, Anna; Mir, Gisela; Pla, María; Verdaguer, Dolors; Huguet, Gemma; Molinas, Marisa

doi:10.1093/jxb/53.373.1445

Cited by 10 publications

(14 citation statements)

References 30 publications

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“…As shown by the two-dimensional immunodetection patterns, in the holm oak, the anti-QsHsp17.4-CI antibody recognized two sets of immunospots in the 17 and approximately 10 kDa regions, respectively. Similar twodimwnsional patterns have been detected in Q. suber from cork and wood tissues and also in mature somatic embryos and plantlets (Puigderrajols et al 2002;Jofré et al 2003). Polypeptides in the 17 kDa region were induced by heat stress, whereas polypeptides in the 10 kDa region were specifically induced by oxidative stress (Puigderrajols et al 2002;Jofré et al 2003).…”

Section: Expression Of Different Shsps In Leaves and Stemssupporting

confidence: 74%

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Expression of low molecular weight heat‐shock proteins and total antioxidant activity in the Mediterranean tree Quercus ilex L. in relation to seasonal and diurnal changes in physiological parameters

Verdaguer

Aranda

Jofré

et al. 2003

Plant Cell & Environment

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The total antioxidant activity (TAA) and the accumulation of small heat shock proteins (sHsps) were analysed under field conditions in Quercus ilex with regard to organ ontogeny and the physiological state of the plant. The results point toward the participation of sHsps and an increase of TAA in the general adaptation syndrome (GAS) of woody Mediterranean evergreens. In leaves and stems, there was a definite TAA seasonal pattern but no effect from diurnal variations or from the current stage of organ ontogeny. TAA was about twice as large in summer as in spring and winter and the hydrophilic antioxidant content was about 16 times greater than that of lipophilic antioxidants. The accumulation of sHsps in leaves also showed a seasonal pattern, but no effect from diurnal variations or from leaf ontogeny. In summer days, the sHsps content remained invariable even during the daylight hours in which the leaves were physiologically recovered. However, the accumulation of sHsps in stems did vary in relation to organ ontogeny. Old stems had a high accumulation of sHsps throughout the year, whereas in young stems, accumulation of sHsps was detected only in summer. This is probably due to a higher quantity of lignified and suberized tissues in the older stems. Using two-dimensional immunodetection for leaves and stems, two sets of Hs protein species (17 and 10 kDa regions) were observed. In stems, there was an increase in 10 kDa proteins from winter to summer. These results are discussed and the possible role of the two types of polypeptides in the face of environmental and endogenous oxidative stress are considered.

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Section: Expression Of Different Shsps In Leaves and Stemssupporting

confidence: 74%

“…1998) and to certain development cues, such as embryo development (e.g. Wehmeyer & Vierling 2000;Puigderrajols et al . 2002) and seed maturation (Hernandez & Vierling 1993).…”

Section: Introductionmentioning

confidence: 99%

Expression of low molecular weight heat‐shock proteins and total antioxidant activity in the Mediterranean tree Quercus ilex L. in relation to seasonal and diurnal changes in physiological parameters

Verdaguer

Aranda

Jofré

et al. 2003

Plant Cell & Environment

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“…Developmental induction of Ha HSP17.7 G4 during zygotic embryogenesis was faithfully reproduced in the transgenic plants (Coca et al, 1996). Puigderrajols et al (2002) reported a transient accumulation of class I sHSPs during cork oak somatic embryo maturation and germination. This was localized to early differentiating, excluding the highly dividing regions of the root and shoot apical meristems.…”

Section: Shsp and Developmentmentioning

confidence: 85%

An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

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In the last 25 years, a huge amount of literature has been accumulated describing the cell's response to different kinds of environmental stress conditions, such as high temperatures, altered pH, exposure of the cell to toxins, starvation, oxygen, and water deprivation, among others. Heat shock proteins (HSPs) are one of the main expressed products of the cell in response to stresses. HSPs can be classified into six structurally conserved classes according to their molecular weight namely, HSP100, HSP90, HSP70, HSP60, small heat shock proteins (sHSPs) and ubiquitin (8.5 kDa). In eukaryotes, different heat shock genes are expressed uncoordinatedly, whereas in prokaryote, heat shock genes form a regulon and appear simultaneously. sHSPs are associated with nuclei, cytoskeleton and membranes. They bind partially to denatured proteins, preventing irreversible protein aggregation during stress. In animals, only one sHSP gene has been located in yeast cells, ten in mammalian, two in birds and four genes have been found in Drosophila. However, in plants more than 20 sHSPs have been reported and they can be divided into 6 classes, of which, 3 classes (CI, CII and CIII) are in the cytosole or in the nucleus and the other three (CIV, CV and CVI) in the plastids, endoplasmic reticulum and mitochondria. Mitochondrial and chloroplast sHSPs protect electron transport chain. During development in animals, sHSP genes are normally regulated at late neurula and early tailbud stage and in plants during pollen development, seed maturation, seed imbibition and germination. Transcriptional regulation of sHSPs depends on particular activation of heat shock factors (HSF) which recognize the highly conserved heat-shock elements (HSEs). After the heat stress has been released, the sHSPs are quite stable, suggesting that sHSPs may be important for recovery as well.

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“…There have been numerous studies concerning the hormonal induction of SE in a wide range of species. A significant amount of literature on auxin biosynthesis, metabolism, and transport in embryos has grown out of extensive analysis which shows that auxin plays important roles both in induction of embryo formation in culture and in the subsequent elaboration of proper morphogenesis during embryo development [42,149]. One of the well-established functions of the plant ubiquitin/ proteasome pathway is in auxin signaling as illustrated in Fig.…”

Section: Pgrsmentioning

confidence: 99%

Molecular aspects of somatic-to-embryogenic transition in plants

2009

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Somatic embryogenesis (SE) is a model system for understanding the physiological, biochemical, and molecular biological events occurring during plant embryo development. Plant somatic cells have the ability to undergo sustained divisions and give rise to an entire organism. This remarkable feature is called plant cell totipotency. SE is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Plant growth regularities, especially auxins, are key components as their exogenous application recapitulates the embryogenic potential of the mitotically quiescent somatic cells. It has been observed that there are genetic and also physiological factors that trigger in vitro embryogenesis in various types of plant somatic cells. Analysis of the proteome and transcriptome has led to the identification and characterization of certain genes involved in SE. Most of these genes, however, are upregulated only in the late developmental stages, suggesting that they do not play a direct role in the vegetative-to-embryogenic transition. However, the molecular bases of those triggering factors and the genetic and biochemical mechanisms leading to in vitro embryogenesis are still unknown. Here, we describe the plant factors that participate in the vegetative-to-embryogenic transition and discuss their possible roles in this process.

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Developmentally and stress‐induced small heat shock proteins in cork oak somatic embryos

Cited by 10 publications

References 30 publications

Expression of low molecular weight heat‐shock proteins and total antioxidant activity in the Mediterranean tree Quercus ilex L. in relation to seasonal and diurnal changes in physiological parameters

Expression of low molecular weight heat‐shock proteins and total antioxidant activity in the Mediterranean tree Quercus ilex L. in relation to seasonal and diurnal changes in physiological parameters

An overview on the small heat shock proteins

Molecular aspects of somatic-to-embryogenic transition in plants

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