Developmentofin-vitroSensitivityTestingforPathogenicBacteria

Abstract

Anewmethod developed for in vitrosusceptibility test in medicall aboratoriesconsistofmicrotubesorglovescontainingdehydratedtrypticsoyabroth,5%glucose,0.1%bromothymolblueandonetypeofantibiotics(ampicillin,tetracyclineandchloramphenicol)withcriticalconcentrationMIC(minimuminhibitoryconcentration)forsusceptibility.Standardqualitycontrolstrainsofbacterial(Escherichiacoli,StaphylococcusaureusandPseudomonasaeruginosa)suspensionwereadjustedto0.5McFarlandturbiditystandard(1×106cell/mL)wereusedininoculationthemediaandincubatedtwohoursat37°C.TheMICofampicillinagainstE.coli,S.aureus,andP.aeruginosawere4,32,and256µg/mLofthemediaforthebacteriarespectively,whiletheMICoftetracyclineagainstbacteriawere512,512and32µg/mLrespectively,theMICofchloramphenicolwere512,32and512µg/mL,respectively.Where,theresistantbacteriatotheantibioticscouldgrowandfermentglucosesugarproducingacolorchangeofthemediafrombluetoyellow,whilethesensitivebacteriadonotgroworshownochangeincolor.Ourstudyresultcomparedwithcommonusedantibioticdiskmethodobtainingsimilarresults.Thisdevelopedmethodcharacterizedbyfast(onlytwohours)andlesscostincomparisontoconventionaltechnique.Thenewmicrotubestripishighlystable(morethanoneyear)withmoresensitiveindetectionofvariablepathogenicbacteriaincludingstandardbacteriastrainscomparedwithconventionaltechnique.

Study of Ashes from Pachystroma longifolium Leaf as Stabilizer For PVC Exposed to γ‐Irradiation

“…In the last years, natural fillers such as wood flour, coconut shell, kenaf bast, pineapple leaf, sansevieria leaf, abaca leaf, flax, sisal, jute, banana bamboo, date, palm, and sugarcane fibers have been used as reinforcement in polymer composites that are competitive with synthetic composites. [1,2] Natural fillers have attracted the interest of researchers and have experienced a rapid expansion over the last decade, due to the advantageous features, i.e., abundance, environmental friendliness, biodegradability, nontoxicity, low cost and density, flexibility during processing, and fairly good mechanical properties. [3,4] Poly(vinyl chloride) (PVC) is a common thermoplastic that has been used as polymer matrix in reinforced composites with natural fillers.…”