Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides: 2. Field Research on Bioremediation of Metals and Radionuclides

Hazen, Terry C.; Tabak, Henry H.

doi:10.1007/s11157-005-2170-y

Cited by 55 publications

(45 citation statements)

References 51 publications

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“…The concentration of complexed Cu was shown to increase and the free Cu 2+ decreased in the soil solution following the mulch treatment. Hazen & Tabak (2005) give a detailed presentation of field study reporting metals immobilization with biosolids.…”

Section: Complexationmentioning

confidence: 99%

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides. 3. Influence of Chemical Speciation and Bioavailability on Contaminants Immobilization/Mobilization Bio-processes

Hullebusch

Lens

Tabak

2005

Rev Environ Sci Biotechnol

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The biotransformation of metals is an exciting, developing strategy to treat metal contamination, especially in environments that are not accessible to other remediation technologies. However, our ability to benefit from these strategies hinges on our ability to monitor these transformations in the environment. That's why remediation of contaminated sediments and soil requires detailed in situ characterization of the speciation of the toxic substances and their transformations with respect to time and spatial distribution. The present paper gives an overview of the literature regarding research performed in the laboratory as well as in the field.

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Section: Complexationmentioning

confidence: 99%

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides. 3. Influence of Chemical Speciation and Bioavailability on Contaminants Immobilization/Mobilization Bio-processes

Hullebusch

Lens

Tabak

2005

Rev Environ Sci Biotechnol

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“…Uranium contamination in subsurface environments is a widespread problem at numerous US Department of Energy (DOE) sites (Hazen et al 2005;Marshall et al 2009), and its fate and transport are largely influenced by its oxidation state (oxidized: U(VI) or reduced: U(IV)). Uranium can be immobilized in groundwater systems through the reduction of soluble U(VI) to insoluble U(IV) by indirect and direct (enzymatic) processes catalyzed by dissimilatory metal-and sulfate-reducing bacteria that are stimulated by the injection into the subsurface of an electron donor (Anderson et al 2003;Lloyd et al 2000;Lovley et al 1991;Lovley and Phillips 1992;Lovley 1995;Wall and Krumholz 2006;Gorby and Lovely 1992).…”

Section: Introductionmentioning

confidence: 99%

Uranium reduction and microbial community development in response to stimulation with different electron donors

Barlett

Moon

Peacock³

et al. 2012

Biodegradation

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Stimulating microbial reduction of soluble U(VI) to less soluble U(IV) shows promise as an in situ bioremediation strategy for uranium contaminated groundwater, but the optimal electron donors for promoting this process have yet to be identified. The purpose of this study was to better understand how the addition of various electron donors to uraniumcontaminated subsurface sediments affected U(VI) reduction and the composition of the microbial community. The simple electron donors, acetate or lactate, or the more complex donors, hydrogen-release compound (HRC) or vegetable oil, were added to the sediments incubated in flow-through columns. The composition of the microbial communities was evaluated with quantitative PCR probing specific 16S rRNA genes and functional genes, phospholipid fatty acid analysis, and clone libraries. All the electron donors promoted U(VI) removal, even though the composition of the microbial communities was different with each donor. In general, the overall biomass, rather than the specific bacterial species, was the factor most related to U(VI) removal. -Vegetable oil and HRC were more effective in stimulating U(VI) removal than acetate. These results suggest that the addition of more complex organic electron donors could be an excellent option for in situ bioremediation of uranium-contaminated groundwater.

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“…For example, lactate is a convenient electron donor for promoting anaerobic metabolism because it can be provided from onetime emplacements of slow-release formulations (Hazen and Tabak, 2005). In contrast to acetate, which is a major intermediate in carbon and electron flow in the subsurface, lactate is expected to be a minor constituent under conditions present during the stimulation of bioremediation in the subsurface (Lovley and Chapelle, 1995).…”

Section: Introductionmentioning

confidence: 99%

Laboratory evolution of Geobacter sulfurreducens for enhanced growth on lactate via a single-base-pair substitution in a transcriptional regulator

et al. 2011

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The addition of organic compounds to groundwater in order to promote bioremediation may represent a new selective pressure on subsurface microorganisms. The ability of Geobacter sulfurreducens, which serves as a model for the Geobacter species that are important in various types of anaerobic groundwater bioremediation, to adapt for rapid metabolism of lactate, a common bioremediation amendment, was evaluated. Serial transfer of five parallel cultures in a medium with lactate as the sole electron donor yielded five strains that could metabolize lactate faster than the wild-type strain. Genome sequencing revealed that all five strains had non-synonymous single-nucleotide polymorphisms in the same gene, GSU0514, a putative transcriptional regulator. Introducing the single-base-pair mutation from one of the five strains into the wild-type strain conferred rapid growth on lactate. This strain and the five adaptively evolved strains had four to eight-fold higher transcript abundance than wild-type cells for genes for the two subunits of succinyl-CoA synthase, an enzyme required for growth on lactate. DNA-binding assays demonstrated that the protein encoded by GSU0514 bound to the putative promoter of the succinyl-CoA synthase operon. The binding sequence was not apparent elsewhere in the genome. These results demonstrate that a single-base-pair mutation in a transcriptional regulator can have a significant impact on the capacity for substrate utilization and suggest that adaptive evolution should be considered as a potential response of microorganisms to environmental change(s) imposed during bioremediation.

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Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides: 2. Field Research on Bioremediation of Metals and Radionuclides

Cited by 55 publications

References 51 publications

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides. 3. Influence of Chemical Speciation and Bioavailability on Contaminants Immobilization/Mobilization Bio-processes

Developments in Bioremediation of Soils and Sediments Polluted with Metals and Radionuclides. 3. Influence of Chemical Speciation and Bioavailability on Contaminants Immobilization/Mobilization Bio-processes

Uranium reduction and microbial community development in response to stimulation with different electron donors

Laboratory evolution of Geobacter sulfurreducens for enhanced growth on lactate via a single-base-pair substitution in a transcriptional regulator

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