Developments in mycotoxin analysis: an update for 2013-2014

Berthiller, Franz; Brera, Carlo; Crews, Colin; Iha, Maria Helena; Krsha, R.; Lattanzio, Veronica M.T.; MacDonald, Susan; Malone, R.J.; Maragos, Chris M.; Solfrizzo, Michele; Stroka, J; Whitaker, T. B.

doi:10.3920/wmj2014.1840

Cited by 41 publications

(24 citation statements)

References 205 publications

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“…Due to its prevalence and importance, several analytical methods have been developed and extensively reviewed for the quantitative determination of OTA in foodstuffs [6,7,8,9]. These methods, which allow the detection of OTA alone or in combination with other mycotoxins, are mainly based on high-performance liquid chromatography (HPLC) coupled to fluorescence or mass spectrometry detection.…”

Section: Introductionmentioning

confidence: 99%

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

McKeague

Velu

Girolamo

et al. 2016

Toxins

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Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.

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Section: Introductionmentioning

confidence: 99%

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

McKeague

Velu

Girolamo

et al. 2016

Toxins

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“…The enormous advances of analytical methods and the development of multi-toxin methods have however enabled the detection of a large number of other fungal metabolites. The potential toxicological relevance of these metabolites must therefore be established, and if necessary, appropriate legislative regulations must be put in place [3,4]. The sensitive simultaneous detection of multiple fungal metabolites via liquid chromatography-tandem mass spectroscopy (LC-MS/MS) [5], has aided the discovery of new fungal secondary metabolites, so called “emerging mycotoxins”.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Springler

Vrubel²,

Mayer³

et al. 2016

Toxins

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The human, animal and plant pathogen Fusarium, which contaminates agricultural commodities worldwide, produces numerous secondary metabolites. An example is the thoroughly-investigated deoxynivalenol (DON), which severely impairs gastrointestinal barrier integrity. However, to date, the toxicological profile of other Fusarium-derived metabolites, such as enniatins, beauvericin, moniliformin, apicidin, aurofusarin, rubrofusarin, equisetin and bikaverin, are poorly characterized. Thus we examined their effects—as metabolites alone and as metabolites in combination with DON—on the intestinal barrier function of differentiated intestinal porcine epithelial cells (IPEC-J2) over 72 h. Transepithelial electrical resistance (TEER) was measured at 24-h intervals, followed by evaluation of cell viability using neutral red (NR) assay. Enniatins A, A1, B and B1, apicidin, aurofusarin and beauvericin significantly reduced TEER. Moniliformin, equisetin, bikaverin and rubrofusarin had no effect on TEER. In the case of apicidin, aurofusarin and beauvericin, TEER reductions were further substantiated by the addition of otherwise no-effect DON concentrations. In all cases, viability was unaffected, confirming that TEER reductions were not due to compromised viability. Considering the prevalence of mycotoxin contamination and the diseases associated with intestinal barrier disruption, consumption of contaminated food or feed may have substantial health implications.

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“…Moreover, new developments and updates in this field are covered on a yearly basis in World Mycotoxin Journal [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

130

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Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

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Developments in mycotoxin analysis: an update for 2013-2014

Cited by 41 publications

References 205 publications

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

Effect of Fusarium-Derived Metabolites on the Barrier Integrity of Differentiated Intestinal Porcine Epithelial Cells (IPEC-J2)

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

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