Developments in our understanding of the effects of growth hormone on white adipose tissue from mice: implications to the clinic

Berryman, Darlene E.; Henry, Brooke E.; Hjortebjerg, Rikke; List, Edward O.; Kopchick, John J.

doi:10.1586/17446651.2016.1147950

Cited by 8 publications

(2 citation statements)

References 90 publications

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“…GH, IGF-1, and insulin signaling are essential for AT development and function (26,37). Thus, we investigated the RNA levels of Igf1, Igf1r, Ir, and Ghr in AT as well as levels of IGF-1R, IR, and GHR protein in Sc in WT, bGH, and GHR-/-mice.…”

Section: Discussionmentioning

confidence: 99%

Insulin, IGF-1, and GH Receptors Are Altered in an Adipose Tissue Depot–Specific Manner in Male Mice With Modified GH Action

et al. 2017

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Growth hormone (GH) is a determinant of glucose homeostasis and adipose tissue (AT) function. Using 7-month-old transgenic mice expressing the bovine growth hormone (bGH) gene and growth hormone receptor knockout (GHR-/-) mice, we examined whether changes in GH action affect glucose, insulin, and pyruvate tolerance and AT expression of proteins involved in the interrelated signaling pathways of GH, insulinlike growth factor 1 (IGF-1), and insulin. Furthermore, we searched for AT depot-specific differences in control mice. Glycated hemoglobin levels were reduced in bGH and GHR-/- mice, and bGH mice displayed impaired gluconeogenesis as judged by pyruvate tolerance testing. Serum IGF-1 was elevated by 90% in bGH mice, whereas IGF-1 and insulin were reduced by 97% and 61% in GHR-/- mice, respectively. Igf1 RNA was increased in subcutaneous, epididymal, retroperitoneal, and brown adipose tissue (BAT) depots in bGH mice (mean increase ± standard error of the mean in all five depots, 153% ± 27%) and decreased in all depots in GHR-/- mice (mean decrease, 62% ± 4%). IGF-1 receptor expression was decreased in all AT depots of bGH mice (mean decrease, 49% ± 6%) and increased in all AT depots of GHR-/- mice (mean increase, 94% ± 8%). Insulin receptor expression was reduced in retroperitoneal, mesenteric, and BAT depots in bGH mice (mean decrease in all depots, 56% ± 4%) and augmented in subcutaneous, retroperitoneal, mesenteric, and BAT depots in GHR-/- mice (mean increase: 51% ± 1%). Collectively, our findings indicate a role for GH in influencing hormone signaling in AT in a depot-dependent manner.

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Section: Discussionmentioning

confidence: 99%

Insulin, IGF-1, and GH Receptors Are Altered in an Adipose Tissue Depot–Specific Manner in Male Mice With Modified GH Action

et al. 2017

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“…In terms of lipolytic effects there are several studies supporting the concept that GH stimulates lipolysis by increasing the activity of the adipose tissue lipases HSL and ATGL [27][28][29][30]. The characterization of MGL revealed its specific mode of action as an enzyme for the hydrolysis of monoacylglycerol in adipose tissue and the occurrence in other tissues, e. g. testis or kidney [21,24].…”

Section: Gh and Lipolysismentioning

confidence: 96%

Short-Term Effects of Growth Hormone on Lipolysis, Glucose and Amino Acid Metabolism Assessed in Serum and Microdialysate of Healthy Young Men

Krebs

Baum

Doerfer

et al. 2019

Exp Clin Endocrinol Diabetes

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Objective We investigated direct effects of a therapeutic growth hormone dose on lipolysis, glucose and amino acid metabolism. Methods This crossover microdialysis trial involved six healthy male volunteers receiving single subcutaneous injections of both growth hormone (0.035 mg/kg) and placebo (0.9% sodium chloride). The investigation comprised three test days with standard diet. The first day served for adaptation, the second and third one for determining study data during 9 night hours with or without growth hormone. Abdominal subcutaneous microdialysate and blood were continuously collected and forwarded to a separate room next door where hourly taken samples were centrifuged and frozen until analysed. Results Growth hormone achieved the peak serum level after 3 h followed by a plateau-like course for the next 6 h. Glycerol in microdialysate started to rise 2 h following growth hormone injection achieving significance compared to placebo after 9 h (P<0.05). Serum glycerol increased 4 h after growth hormone administration achieving significance after 6 h (P<0.05). Glucose and amino acid concentrations showed neither in microdialysate nor in serum significant differences between growth hormone and placebo. Serum values of insulin and C-peptide revealed no significant difference between growth hormone and placebo. Summary and Conclusion As the result of a high single subcutaneous dose of GH, persistent lipolysis can be shown in continuously collected microdialysate and blood, but no indication for gluconeogenesis or protein anabolism.

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Growth Hormone (GH)

Hjortebjerg¹,

Baumann²,

Kopchick³

2017

Encyclopedia of Endocrine Diseases

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Developments in our understanding of the effects of growth hormone on white adipose tissue from mice: implications to the clinic

Cited by 8 publications

References 90 publications

Insulin, IGF-1, and GH Receptors Are Altered in an Adipose Tissue Depot–Specific Manner in Male Mice With Modified GH Action

Insulin, IGF-1, and GH Receptors Are Altered in an Adipose Tissue Depot–Specific Manner in Male Mice With Modified GH Action

Short-Term Effects of Growth Hormone on Lipolysis, Glucose and Amino Acid Metabolism Assessed in Serum and Microdialysate of Healthy Young Men

Growth Hormone (GH)

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