Developments of pulse laser assist optical tweezers (PLAT) for in vivo manipulation

Maeda, Saki; Sugiura, T.; Minato, Kotaro

doi:10.1117/12.874647

Cited by 2 publications

(4 citation statements)

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“…According to this, we may suppress eruption of laser induced breakdown by adjusting the laser power to appropriate value. Also pulse elongation has been confirmed to be efficient to prevent LIB due to reduction of maximum power of pulse laser [9] . Here we discuss relationships between pulse width and prevention of LIB, according to a series of experiments on LIB eruption with a pulse-duration variable long pulse laser, and we reveal relationship between laser power and eruption of LIB.…”

Section: Improvement Of Plat Experimentsmentioning

confidence: 97%

“…We have already developed pulse laser assist optical tweezers (PLAT) system to realize in vivo manipulation of a cell by generating strong instantaneous force with a pulse laser beam [9,10] . In our PLAT system a beam from a continuous wave (CW) laser (Nd:YVO 4 , 1064-nm wavelength, 2-W max power, Spectra-Physics, Inc.) is irradiated for conventional optical tweezers, and also a beam from a pulse laser (Q-switched pulse Nd: YAG laser, 1064-nm wavelength, 6 ns or 150 μs pulse durations, Spectron) is introduced for pulse assist.…”

Section: Experimental Setup Of Plat and Plat Experimentsmentioning

confidence: 99%

“…We have estimated a suitable pulse duration as 100 ns to 200 ns in case of 1-micron diameter particle in water according to the movement of particle in strong instantaneous force [9] .…”

Section: 4)mentioning

confidence: 99%

“…This method is also applied to cell manipulation experiment. We have performed to extract particles adhered on mouse fibroblast cell Balb 3T3 and have succeeded at scission of lipid tether formed between the particle and cell membrane [9,11] . (a) A CW Nd: YAG laser beam is introduced onto a particle adsorbed on glass substrate.…”

Section: Particle Extraction Experimentsmentioning

confidence: 99%

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Pulse laser assist optical tweezers (PLAT) with long-duration pulse laser

2011

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Optical tweezers is a technique to trap and to manipulate micron sized objects under a microscope by radiation pressure force exerted by a laser beam. Optical tweezers has been utilized for single-molecular measurements of force exerted by molecular interactions and for cell palpation. To extend applications of optical tweezers we have developed a novel optical tweezers system combined with a pulse laser. We utilize a pulse laser (Q-switched Nd: YAG laser, wavelength of 1064 nm) to assist manipulations by conventional optical tweezers with a continuous wave (CW) laser. The pulse laser beam is introduced into the same optics for conventional optical tweezers. In principle, instantaneous radiation force is proportional to instantaneous power of laser beam. As a result, pulse laser beam generates strong instantaneous force on an object to be manipulated. If the radiation force becomes strong enough to get over an obstacle structure and/or to be released from adhesion, the object will be free from these difficulties. We investigate the effect of pulse laser assistance with changing pulse duration of the laser. We report optimum pulse duration of 100 ns to 200 ns deduced from motion analysis of a particle in a beam spot. Our goal is to realize in-vivo manipulation and operation of a cell. For this purpose we need to reduce light energy of pulse laser beam and to avoid laser induced breakdown caused by strong light field. So we have developed a pulse laser with 160-ns pulse duration and have confirmed that availability on manipulation of living cells.

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Section: Improvement Of Plat Experimentsmentioning

confidence: 97%

Section: Experimental Setup Of Plat and Plat Experimentsmentioning

confidence: 99%

“…We have estimated a suitable pulse duration as 100 ns to 200 ns in case of 1-micron diameter particle in water according to the movement of particle in strong instantaneous force [9] .…”

Section: 4)mentioning

confidence: 99%

Section: Particle Extraction Experimentsmentioning

confidence: 99%

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