Développement de la Filaire<i>Monanema martini</i>dans l’épiderme des tiques Ixodidae

Petit, G.; Bain, O.; Carrat, Christophe; Marval, F. de

doi:10.1051/parasite/198863154

Cited by 13 publications

(7 citation statements)

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“…Since the elevation of Cercopithifilaria Eberhard, 1980 to the genus rank by Bain et al (1982 a ), 28 species parasitizing primates, ungulates, rodents, carnivores and marsupials have been classified or described within this genus (Bain et al 2002). Cercopithifilaria adults are usually localized beneath cutaneous tissues, whereas microfilariae are always in the dermis (Bain et al 2002), the infestation being transmitted by ixodid ticks (Ixodida, Ixodidae) (Winkhardt, 1980; Bain et al 1986; Spratt and Haycock, 1988; Petit et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region

et al. 2012

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Following the recent description of microfilariae of a Cercopithifilaria sp. in a dog from Sicily, Italy, (herein after referred to as Cercopithifilaria sp. I), numerous skin samples were collected from dogs in the Mediterranean region. In addition to Cercopithifilaria sp. I (185·7 ± 7·2 μm long), microfilariae of 2 other species were identified, namely Cercopithifilaria grassii (651·7 ± 23·6 μm long) and a yet undescribed microfilaria, Cercopithifilaria sp. II (264·4 ± 20·2 μm long, with evident lateral alae). The morphological differentiation among the 3 species of dermal microfilariae was confirmed by differences in cytochrome c oxidase subunit 1 and ribosomal 12S sequences examined (mean level of interspecific pairwise distance of 11·4%, and 17·7%, respectively). Phylogenetic analyses were concordant in clustering these with other sequences of Cercopithifilaria spp. to the exclusion of Dirofilaria spp., Onchocerca spp. and Acanthocheilonema spp. Dermal microfilariae collected (n = 132) were morphologically identified as Cercopithifilaria sp. I (n = 108, 81·8%), Cercopithifilaria sp. II (n = 17, 12·9%), whereas only 7 (5·3%) were identified as C. grassii. Mixed infestations were detected in all sites examined. The great diversity of these neglected filarioids in dogs is of biological interest, considering the complex interactions occurring among hosts, ticks and Cercopithifilaria spp. in different environments.

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Section: Introductionmentioning

confidence: 99%

Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region

et al. 2012

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“…Monanema martini is a filarial nematode identified in Africa that infects at least two species of African rodents, the typical striped grass mouse ( Lemniscomys striatus ), and the African grass rat ( Arvicanthis niloticus ) with Hyalomma and Rhipicephalus ticks implicated as vectors [ 7 , 8 ]. We used consensus PCR to obtain sequences of a 455 nt fragment of the ISN 12S rRNA gene that is often used for phylogenetic assignment of Filarioidea.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Monanema nematode in Ixodes scapularis

et al. 2020

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Background: Metagenomic studies have revealed the presence of a filarial nematode in Ixodes scapularis. The phylogeny of this agent, and its potential for human infection, are unknown. Methods: We used existing metagenomic data from I. scapularis to determine the phylogeny of this tick-associated nematode and employed quantitative PCR to determine if the presence of this agent had an effect on the burden of Borrelia burgdorferi. We also developed a Luciferase Immunoprecipitation System assay using the Av33 antigen as a target to investigate the presence of antibodies against this nematode in 128 serum specimens from patients with Lyme disease and babesiosis. To demonstrate assay utility, we used 15 sera from patients with onchocerciasis as controls. Results: We show that this agent is a new species in the genus Monanema and its presence in vector ticks does not impact the burden of B. burgdorferi. We did not detect IgG antibodies to this agent in 127 of 128 sera from patients with Lyme disease or babesiosis. One sample had reactivity above the threshold, but at the low-level equivalent to the least reactive onchocerciasis sera. This low positive signal could be a result of cross-reacting antibodies, antibodies from a previous infection with a filarial nematode, or, less likely, a exposure to the Ixodes scapularis-associated nematode. Conclusions: We found no evidence that this nematode contributes to the spectrum of human tick-borne infections.

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“…Homology searches of the 18S rRNA gene (accession number MK868471) revealed 100% and 98.9% matches to sequences KP760148 and KP760149 that correspond to a 665-nucleotide fragment of 18S rRNA from Monanema martini. M. martini is a filarial nematode identified in Africa that infects at least two species of African rodents, the typical grass striped mouse (Lemniscomys striatus), and the African grass rat (Arvicanthis niloticus) with Hyalomma and Rhipicephalus ticks implicated as vectors [7,8]. We also used consensus PCR to obtain sequences of a 455 nt fragment of the ISN 12S rRNA gene that is often used for phylogenetic assignment of Filarioidea.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Monanema nematode in Ixodes scapularis

Tokarz

Tagliafierro

Lipkin

et al. 2020

Preprint

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Background Metagenomic studies have revealed the presence of a filarial nematode in Ixodes scapularis. The phylogeny of this agent, and its potential for human infection, are unknown.Methods We used existing metagenomic data from I. scapularis to determine the phylogeny of this tick-associated nematode and employed quantitative PCR to determine if the presence of this agent had an effect on the burden of Borrelia burgdorferi. We also developed a Luciferase Immunoprecipitation System assay using the Av33 antigen as a target to investigate the presence of antibodies against this nematode in 128 serum specimens from patients with Lyme disease and babesiosis. To demonstrate assay utility, we used 15 sera from patients with onchocerciasis as controls.Results We show that this agent is a new species in the genus Monanema and its presence in vector ticks does not impact the burden of B. burgdorferi. We did not detect IgG antibodies to this agent in 127 of 128 sera from patients with Lyme disease or babesiosis. One sample had reactivity above the threshold, but at the low-level equivalent to the least reactive onchocerciasis sera. This low positive signal could be a result of cross-reacting antibodies, antibodies from a previous infection with a filarial nematode, or, less likely, a exposure to the Ixodes scapularis-associated nematode.Conclusions We found no evidence that this nematode contributes to the spectrum of human tick-borne infections.

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Développement de la FilaireMonanema martinidans l’épiderme des tiques Ixodidae

Cited by 13 publications

References 4 publications

Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region

Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region

Characterization of a Monanema nematode in Ixodes scapularis

Characterization of a Monanema nematode in Ixodes scapularis

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