Devising a multi epitope vaccine toward the dengue virus using the computational method in Bangladesh

Abstract

Dengue is one of the major mosquito-borne diseases that still threaten humans and kill countless people. It is caused by a positive-stranded RNA virus that included the family of Flaviviridae. Dengue fever is an exquisite feverish viral disease dispatched by Aedes mosquitoes' sting, posing any one of the four dengue viral serotypes. This virus transmits through a vertical transmission way using a full unique system. Unfortunately, there is no still effective developed vaccine to eradicate this disease. Computational methods were used in this work to build and we suggest a multi-epitope vaccination for the dengue virus in Asia. This work employed different immunoinformatics and bioinformatics databases to predict powerful epitopes on the dengue virus envelope protein utilizing in silico techniques. We identified a total of 14 epitopes from the target envelope protein by evaluating their ability to induce both innate and acquired immunity via T and B lymphocyte-mediated reactions. Epitope conservation was taken into account because Dengue virus is an RNA virus, and all selected epitopes were 100% conserved. Antigenicity of the final multi-epitope vaccine component was 0.7055. Disulfide engineering was conducted at an area of high mobility to improve vaccine protein stability. In addition, codon adaptation and in silico cloning were used to guarantee that the planned subunit vaccine in E. coli was expressed at a greater level. Lastly, a molecular docking and simulation analysis was carried out for the vaccination protein and the TLR-4 receptor in order to assess the binding free energy and stability of the combination for this reason, the suggested in silico vaccine has to be tested for safety and immunogenicity in order to guarantee an active immunity against the Dengue virus.

In vitro pharmacological activities of methanol extract of Acmella oleracea leaves: A variety grown in Dhaka, Bangladesh

“…1 Free radical cell damage has been linked to many different diseases, including cancer, arthritis, atherosclerosis, Alzheimer's, and diabetes. 2 Some evidence shows that cell death mechanisms within the body, such as apoptosis and in severe cases necrosis, are initiated and enhanced by reactive nitrogen species and free radicals. 3 Targeted antioxidants, like mitochondria-targeted ubiquinone, may reduce the damage to the liver caused by drinking too much alcohol and lead to better therapeutic effects.…”