Devising Assisted Reproductive Technologies for Wild-Derived Strains of Mice: 37 Strains from Five Subspecies of Mus musculus

Mochida, Keiichi; Hasegawa, Ayumi; Otaka, Naoki; Hama, Daiki; Furuya, Takashi; Ichikawa, Eri; Ijuin, Maiko; Taguma, Kyuichi; Hashimoto, Michiko; Takashima, Rika; Kadota, Masayo; Hiraiwa, Noriko; Mekada, Kazuyuki; Yoshiki, Atsushi; Ogura, Atsuo

doi:10.1371/journal.pone.0114305

Cited by 35 publications

(36 citation statements)

References 30 publications

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“…Previous studies have compared fertilization success between different strains of mice, but none have included the BTBR strain (Byers et al 2006; Mochida et al 2014; Sztein et al 2000; Vasudevan et al 2010). Although good spontaneous breeders, reproductive success is low in BTBR mice after IVF, manipulation of the genome, and embryo transfer.…”

Section: Discussionmentioning

confidence: 99%

“…Reproductive parameters such as IVF capacity have been examined for several other strains of mice, including the widely used C57BL/6J mouse (Bath 2010; Byers et al 2006; Guan et al 2014; Mochida et al 2014; Ostermeier et al 2008; Takeo et al 2008; Takeo and Nakagata 2011; Vasudevan et al 2010; Vasudevan and Sztein 2011; Vergara et al 1997). However, despite its unique suitability as a model for T2DM and autism, the BTBR mouse has been relatively underutilized for embryo manipulations, and to our knowledge, there are no published data on transgenic model development or IVF success rates in BTBR mice.…”

Section: Introductionmentioning

confidence: 99%

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Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice

et al. 2016

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The Black and Tan Brachyury (BTBR) mouse strain is a valuable model for the study of long-term complications from obesity-induced type 2 diabetes mellitus and autism spectrum disorder. Due to technical difficulties with assisted reproduction, genetically modified animals on this background have previously been generated through extensive backcrossing, which is expensive and time-consuming. We successfully generated two separate transgenic mouse lines after direct zygote microinjection into this background strain. Additionally, we developed in vitro fertilization (IVF) methods for the BTBR mouse. We found low rates of fertilization and implantation in this strain, and identified the BTBR oocyte as the primary culprit of low success with BTBR IVF. We achieved an increase in live born pups from 5.9% to 35.6% with IVF in the BTBR strain by use of BTBR females at a younger age (18–25 days), collection of oocytes 15–17 hours after superovulation, and the use of supplemented fertilization media. This method eliminates the need for time consuming assisted embryo manipulations that are otherwise required for success with BTBR oocytes. This advancement provides an exciting opportunity to directly generate BTBR transgenics and gene-edited mice using both traditional and emerging genomic editing techniques, such as CRISPR/Cas9. These methods also allow effective colony preservation and rederivation with these strains. To our knowledge, this is the first report describing embryo manipulations in BTBR mice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice

et al. 2016

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“…Nonetheless, healthy pups in at least 14 wild-derived strains were obtained for the first time using our improved ET method (Fig. 4) [25]. It is likely that cotransfer with ICR embryos rescues a small number of surviving wild-derived mouse embryos by maintaining pregnancy to term.…”

Section: Towards the Goals Of Artsmentioning

confidence: 94%

“…We also applied this protocol to other wild-derived strains and classified them into AIS-hCG-susceptive and eCG-hCG-susceptive strains. Accordingly, we optimized the superovulation protocols for each wild-derived strain and successfully cryopreserved embryos from 37 of them [25]. Among them, 20 strains were more effectively superovulated with AIS-hCG than with eCG-hCG injections.…”

Section: Superovulationmentioning

confidence: 99%

“…Through our cumulative ART advancements, we have significantly improved the final yields of pups in standard inbred strains as well as in wild-derived strains (Fig. 3) [25]. Generally, the number of offspring produced from one female can be estimated by multiplying the number of oocytes obtained per female, fertilization rates, survival rates after vitrification at the 2-cell stage, and birthrates after ET into the oviducts of pseudopregnant females at Day 1.…”

Section: Towards the Goals Of Artsmentioning

confidence: 99%

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Development of assisted reproductive technologies in small animal species for their efficient preservation and production

Mochida

2020

J. Reprod. Dev.

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Assisted reproductive technologies (ARTs) are widely used in the animal industry, human clinics, and for basic research. In small laboratory animal species such as mice, ARTs are essential for the production of animals for experiments, the preservation of genetic resources, and for the generation of new strains of genetically modified animals. The RIKEN BioResource Research Center (BRC) is one of the largest repositories of such animal bioresources, and maintains approximately 9,500 strains of mice with a variety of genetic backgrounds. We have sought to devise ARTs specific to the reproductive and physiological characteristics of each strain. Such ARTs include superovulation, in vitro fertilization (IVF), the cryopreservation of embryos and spermatozoa, transportation of cryopreserved materials and embryo transfer (ET). Of these, superovulation likely has the most influence on animal production because it determines the quantity of starting material for other ARTs. Superovulation using anti-inhibin serum combined with estrous synchronization has resulted in approximately a threefold increase in production efficiency with IVF-ET in the C57BL/6J strain. Wild-derived strains are important as genetically diverse resources for murine rodents (Genus Mus), and many are unique to the BRC. We have also successfully developed ARTs for more than 50 wild-derived strains, which have been cryopreserved for future use. Our work to improve and develop ARTs for mice and other small laboratory species will contribute to the cost-effectiveness of routine operations at repository centers, and to the provision of high quality animals for research use.

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The History of Transgenesis

Saunders

2019

Methods in Molecular Biology

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Devising Assisted Reproductive Technologies for Wild-Derived Strains of Mice: 37 Strains from Five Subspecies of Mus musculus

Cited by 35 publications

References 30 publications

Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice

Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice

Development of assisted reproductive technologies in small animal species for their efficient preservation and production

The History of Transgenesis

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