Devosia honganensis sp. nov., isolated from the soil of a chemical factory

Abstract

A Gram-negative, strictly aerobic, yellow-pigmented and rod-shaped bacterium, designated strain NSL10(T), was isolated from the waste surface soil of a chemical factory in Hongan, China. Strain NSL10(T) was found to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in modified LB medium. Cells were found to be positive for catalase and oxidase. The G+C content of the total DNA was determined to be 66.8 mol%. The 16S rRNA gene sequence of strain NSL10(T) showed the highest similarity to that of Devosia albogilva IPL15(T) (96.80 %), followed by Devosia geojensis BD-c194(T) (96.46 %) and Devosia chinhatensis IPL18(T) (96.27 %). The major cellular fatty acids of strain NSL10(T) were identified as C18:1 ω7c/C18:1 ω6c (48.2 %) and C16:0 (17.7 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and an unidentified compound. Minor amounts of unidentified glycolipids and unidentified polar lipids were also detected. These chemotaxonomic data supported the affiliation of strain NSL10(T) to the genus Devosia. In conclusion, on the basis of biochemical, physiological characteristics and molecular properties, strain NSL10(T) represents a novel species within the genus Devosia, for which the name Devosia honganensis sp. nov., is proposed. The type strain is NSL10(T) (=KCTC 42281(T) = ACCC 19737(T)).

Dynamics of bacterial and archaeal communities during horse bedding and green waste composting

“…Moreover, two of the three species identified here as having highest relative abundance across all samples, Thermobifida fusca and Thermobifida bifida , agree with the dominant genera, Thermobifida and Thermopolyspora, found by Zhang et al. (2015a) and Zhang et al. (2015b) within maize straw compost .…”

“…Although a range of biotic and abiotic interactions could underlie these microbial shifts, the cellulose degradation associated species increasing in thermophilic Young and Middle phases were largely those with characterized optimal temperatures of 55−60 °C, such as T. composti , B. thermoruber ( Yildiz et al, 2015 ), Paenibacillus barengoltzii , T. curvata and T. chromogena ( Padden et al, 1999 ; Satyanarayana, Kawarabayasi & Littlechild, 2013 ; Watanabe et al, 2007 ; Zainudin et al, 2013 ). Widespread reductions in cellulose degradation associated species occurred from Middle to Aged phase and mature Compost, but still a few species had a higher relative abundance, such as Actinotalea ferrariae , Flavobacterium degerlachei , Lapillicoccus jejuensis, Nocardioides aestuarii and Sorangium cellulosum as well as the Rhizobiales species Devosia honganensis ( Lee & Lee, 2007 ; Li et al, 2013 ; Schneiker et al, 2007 ; Van Trappen et al., 2004 ; Yi & Chun, 2004 ; Zhang et al., 2015b ), some of which have characterized optimal temperatures of <35−40 °C ( A. ferrariae and N. aestuarii ).…”

Devosia ginsengisoli sp. nov., isolated from ginseng cultivation soil

“…Members of the genus are characterized as Gram-stain negative, rod-shaped, aerobic, oxidase-positive bacteria containing ubiquinone-10 (Q-10) or Q-11 as the predominant respiratory quinone with DNA G+C contents ranging from 59 to 65 mol% [3,4]. Various isolation sources have been reported for the genus Devosia including soil root nodule, hexachlorocyclohexan dumpsite, deep-sea sediment and beach sediment [5][6][7][8]. A single study shows that Devosia neptuniae nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India [9].…”

Description of Devosia faecipullorum sp. nov., harboring antibiotic- and toxic compound-resistant genes, isolated from poultry manure

“…[1] in 1996 with Devosia riboflavina as the type species. Species of the genus Devosia have been examined for their bioremediation potential and are known for their presence in habitats contaminated with various toxins, such as diesel-contaminated soil [2], hexachlorocyclohexane dump site [3], soil of a chemical factory [4], surface of a medical leech ( Hirudo verbena ) [5] and other environmental samples. At the time of writing, there are about 28 species of the genus Devosia with validly published names and four members with invalid names have been listed (LPSN; https://lpsn.dsmz.de/genus/devosia).…”