Devosia rhodophyticola sp. nov. and Devosia algicola sp. nov., isolated from a marine red alga

Abstract

Two Gram-stain-negative, obligately aerobic, motile rod bacteria, designated as G2-5T and G20-9T, exhibiting catalase- and oxidase-positive activities, were isolated from the phycosphere of a Chondrus species, a marine red alga. Strain G2-5T exhibited optimal growth at 30 °C and pH 5.0–6.0 and in the presence of 0.5–1.0% NaCl. In contrast, strain G20-9T demonstrated optimal growth at 25 °C and pH 6.0 and in the presence of 0.5–1.5% NaCl. Both strains contained ubiquinone-10, summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C18 : 0 and 11-methyl-C18 : 1 ω7c, and diphosphatidylglycerol and phosphatidylglycerol as the major respiratory isoprenoid quinone, cellular fatty acids and polar lipids, respectively. The genomic DNA G+C contents were 57.2 mol% for strain G2-5T and 57.5 mol% for strain G20-9T. Strains G2-5T and G20-9T exhibited 98.2 % 16S rRNA gene sequence similarity, along with 82.3 % average nucleotide identity (ANI) and 25.0 % digital DNA–DNA hybridization (dDDH) values, indicating that they represent different species. Phylogenetic analyses based on both 16S rRNA gene and genome sequences revealed that strains G2-5T and G20-9T formed distinct phylogenic lineages within the genus Devosia . Strains G2-5T and G20-9T were most closely related to Devosia limi DSM 17137T and Devosia beringensis S02T with 97.7 and 96.9 % 16S rRNA gene sequence similarities, respectively. The ANI and dDDH values between strains G2-5T and G20-9T and other Devosia species were lower than 73.9 and 19.2 %, respectively, suggesting that they constitute novel species within the genus Devosia . Based on their distinct phenotypic, chemotaxonomic, and molecular characteristics, strains G2-5T and G20-9T represent two novel species of the genus Devosia , for which the names Devosia rhodophyticola sp. nov. (G2-5T=KACC 22601T=JCM 35404T) and Devosia algicola sp. nov. (G20-9T=KACC 22650T=JCM 35405T) are proposed, respectively.

Marinomonas rhodophyticola sp. nov. and Marinomonas phaeophyticola sp. nov., isolated from marine algae

“…As previously described [ 11 ], strains KJ51-3 T and 15G1-11 T were isolated in June 2021 from the marine red alga Ahnfeltiopsis flabelliformis and the marine brown alga Sargassum thunbergii , respectively, which were collected from Yangyang-gun (37° 57′ 51.4″ N 128° 45′ 57.6″ E) and Goseong-gun (38° 22′ 22.5″ N 128° 30′ 34.8″ E), respectively, both located in Gangwon Province, Republic of Korea. Briefly, the collected marine algae were gently washed via mechanical vortexing with artificial seawater (ASW; 20 g NaCl, 2.9 g MgSO 4 , 4.5 g MgCl 2 ·6H 2 O, 0.6 g KCl, and 1.8 g CaCl 2 ·2H 2 O per litre), followed by mechanical homogenization using an Ultra-Turrax homogenizer (IKA) for 10 s. The resulting homogenates were serially diluted in ASW, after which 100 µl aliquots from each dilution were spread on marine agar (MA; MBcell).…”

“…Briefly, the collected marine algae were gently washed via mechanical vortexing with artificial seawater (ASW; 20 g NaCl, 2.9 g MgSO 4 , 4.5 g MgCl 2 ·6H 2 O, 0.6 g KCl, and 1.8 g CaCl 2 ·2H 2 O per litre), followed by mechanical homogenization using an Ultra-Turrax homogenizer (IKA) for 10 s. The resulting homogenates were serially diluted in ASW, after which 100 µl aliquots from each dilution were spread on marine agar (MA; MBcell). The agar plates were aerobically incubated at 25 °C for 5 days, and the 16S rRNA genes of colonies grown on MA were amplified via PCR using the 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492R (5′-TACGGYTACCTTGTTACGACTT-3′) universal primers [ 11 ]. PCR amplicons were double-digested with Hae III and Hha I, followed by analysis via 2 % agarose gel electrophoresis.…”

“…PCR amplicons were double-digested with Hae III and Hha I, followed by analysis via 2 % agarose gel electrophoresis. PCR products displaying distinct fragment patterns were partially sequenced using the 340F (5′-CCTACGGGAGGCAGCAG-3′) universal primer [ 11 ], after which the sequences were compared with those of all type strains of validly published species on the EzBioCloud server ( www.ezbiocloud.net/identify ) [ 15 ]. Based on the results from this analysis, two potential novel strains, designated as KJ51-3 T and 15G1-11 T , were selected for further taxonomic characterizations.…”

“…Additionally, these bacteria usually require Na + for growth and possess C 16 : 0 , C 10 : 0 3-OH, summed feature 3 (comprising C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), and summed feature 8 (comprising C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) as their major cellular fatty acids and ubiquinone-8 (Q-8) as their major respiratory quinone [3–10]. In our previous studies on the interactions between marine algae and bacteria, we isolated numerous novel bacteria from marine algae [11–14]. In this study, we isolated and taxonomically characterized two putative novel bacterial strains, designated as strains KJ51-3 T and 15G1-11 T , belonging to the genus Marinomonas , using a polyphasic approach.…”

Coraliomargarita algicola sp. nov., isolated from a marine green alga

“…These bacteria are Gram-negative, non-flagellated cocci, containing menaquinone-7 (MK-7) as the major respiratory quinone, C 18 : 1 ω 9 c , iso-C 14 : 0 , and C 14 : 0 as the major fatty acids, genomic DNA G+C contents ranging from 53.6 to 56.0 mol%, and phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) as major polar lipids [1–4]. In our investigation of interactions between marine algae and bacteria, we have isolated numerous novel bacteria from the phycosphere of marine algae [5–10]. In this particular study, we isolated a strain designated as J2-16 T , belonging to the genus Coraliomargarita , from a marine green alga.…”