Dexamethasone and Dexamethasone Phosphate Detected by1H and19F NMR Spectroscopy in the Aqueous Humour

Midelfart, Anna; Dybdahl, Anne; Müller, Norbert; Sitter, Beathe; Gribbestad, Ingrid S.; Krane, Jostein

doi:10.1006/exer.1997.0429

Cited by 20 publications

(47 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A selective capillary zone electrophoresis (CZE) microassay has also been developed to determine dexamethasone phosphate and its major metabolite dexamethasone in tears after application as an anti-inflammatory drug [4]. The compound has also been detected in aqueous humor by 1 H and 19 F NMR spectroscopy [5]. Amin has worked on the colorimetric determination of corticosteroid drugs (including dexamethasone) using indophenol as a chromophoric reagent [6].…”

Section: Introductionmentioning

confidence: 99%

Trace determination of dexamethasone sodium phosphate in pharmaceutical formulations by differential pulse polarography

Jeyaseelan

Joshi

2002

Anal Bioanal Chem

View full text Add to dashboard Cite

A simple and rapid differential pulse polarographic method has been developed for the trace determination of dexamethasone sodium phosphate. A well-defined single peak with an Ep value of -1.14 V is obtained in acetate buffer (pH 5.0). The linearity is valid in the range 0.2-1.2 mg/25 mL ( r=0.9992) with minimum detection limit of 7.6x10(-6) M. The precision of the method developed is implied from the values of relative mean deviation, standard deviation and coefficient of variation, which are 2.44%, 0.014 and 3.5% respectively. Marketed formulations of dexamethasone sodium phosphate have been analysed by calibration and standard addition methods. Recovery experiments were found to be quantitative, and analysis to determine the mass per tablet was obtained within +/-0.2% of the expected market value. The studies have shown that the method is reproducible and accurate and can be used in the analysis of marketed formulations.

show abstract

Section: Introductionmentioning

confidence: 99%

Trace determination of dexamethasone sodium phosphate in pharmaceutical formulations by differential pulse polarography

Jeyaseelan

Joshi

2002

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…The 1 H spectra of the PCA extracts of the cornea and lens obtained after the treatment of the eyes with dexamethasone phosphate were systematically compared to the spectra of the same tissues from untreated animals [24,25] and to the spectrum of dexamethasone [26]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Hexaflourobenzene (HFB) or 5-fluorouracil (5-FU) was placed in an inner co-axial capillary in the 5 mm NMR tube before the measurement and used as external standards. The peaks were assigned in agreement with previous results [26]. In this article, the spectra presenting fluorine signals show the signal from dexamethasone at 0 ppm.…”

Section: Nmr Spectroscopymentioning

confidence: 94%

“…In this article, the spectra presenting fluorine signals show the signal from dexamethasone at 0 ppm. As demonstrated previously, dexamethasone resonates at 4.7 ppm relatively to 5-FU [26]. Some of the spectra were subjected to quantification.…”

Section: Nmr Spectroscopymentioning

confidence: 99%

“…So far, only limited information about the metabolic change has been obtained, mainly due to problems with suitable detection techniques. Recently, using NMR spectroscopy, both dexamethasone and dexamethasone phosphate have been detected in samples of aqueous humor from rabbit eyes after treatment with dexamethasone phosphate [26]. It has been shown that hydrolyzation occurs either in the cornea or in the aqueous humor.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations