Dexamethasone Attenuates Oncostatin M Production via Suppressing of PI3K/Akt/NF-κB Signaling in Neutrophil-like Differentiated HL-60 Cells

Abstract

Oncostatin M (OSM) plays a role in various inflammatory reactions, and neutrophils are the main source of OSM in pulmonary diseases. However, there is no evidence showing the mechanism of OSM production in neutrophils. While dexamethasone (Dex) has been known to exert anti-inflammatory activity in various fields, the precise mechanisms of OSM downregulation by Dex in neutrophils remain to be determined. Here, we examined how OSM is produced in neutrophil-like differentiated HL-60 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, and Western blot analysis were utilized to assess the potential of Dex. Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation resulted in OSM elevation in neutrophil-like dHL-60 cells. OSM elevation induced by GM-CSF is regulated by phosphatidylinositol 3-kinase (PI3K)/Akt/nuclear factor (NF)-kB signal cascades. GM-CSF stimulation upregulated phosphorylated levels of PI3K or Akt or NF-κB in neutrophil-like dHL-60 cells. Treatment with Dex decreased OSM levels as well as the phosphorylated levels of PI3K or Akt or NF-κB in neutrophil-like dHL-60 cells. Our findings show the potential of Dex in the treatment of inflammatory diseases via blocking of OSM.

Anti-Inflammatory Activity of Geraniol Isolated from Lemon Grass on Ox-LDL-Stimulated Endothelial Cells by Upregulation of Heme Oxygenase-1 via PI3K/Akt and Nrf-2 Signaling Pathways

“…During oxidative stress, in the nucleus, Nrf2 is released from the Keap1-Nrf2 complex. A number of studies have shown that activating the PI3K/AKT signaling pathway can facilitate nuclear translocation of Nrf2 [9,19,20]. Inflammation and induced Ox-LDL may be prevented by activating HO-1 and modulating it.…”

“…The cytoprotective enzyme HO-1 controls inflam have shown that activating Nrf2 can boost HO-1 act working, HO-1 expression is inhibited, and oxidative pressor, Keap1, Nrf2 is inactive in the cytoplasm unde oxidative stress, in the nucleus, Nrf2 is released from th of studies have shown that activating the PI3K/AKT s clear translocation of Nrf2 [9,19,20]. Inflammation and i by activating HO-1 and modulating it.…”

Correction: Han et al. Dexamethasone Attenuates Oncostatin M Production via Suppressing of PI3K/Akt/NF-κB Signaling in Neutrophil-like Differentiated HL-60 Cells. Molecules 2022, 27, 129

“…In the original article [ 1 ], there was a mistake in Figure 1 as published. The authors want to change the time in Figure 1b from 15 min, 30 min, 45 min, 1 h, 2 h, 3 h, and 4 h to 5 min, 10 min, 15 min, 30 min, 1 h, 2 h, and 3 h. The corrected Figure 1 appears below.…”