Dexamethasone-induced differentiation of pancreatic AR42J cell involves p21(waf1/cip1)and MAP kinase pathway

IL-4 inhibits proliferation of renal carcinoma cells by increasing the expression of p21WAF1 and IRF-1

“…WAF1 in response to a genotoxic stress is mediated by p53, cellular accumulation of p21 WAF1 upon TGF-β stimulation or during cellular differentiation and senescence is independent of p53 (Datto et al, 1995;Gartel et al, 1999;Eum et al, 2003). In both cases, the increase of p21 WAF1 correlates with growth arrest, suggesting that it may be the common mediator for different growth inhibitory signals.…”

p21Cip/WAF1 activation is an important factor for the ERK pathway dependent anti-proliferation of colorectal cancer cells

“…The ERK pathway is involved in growth arrest (Liu et al, 1996;Sewing et al, 1997;Woods et al, 1997;Mahyar-Roemer and Roemer, 2001;Park et al, 2002;Boucher et al, 2004) as well as its well known function of growth stimulation (Blenis, 1993;Chang et al, 2003). Activation of p21 Cip/W AF1 , represented by induction and nuclear localization, is an important marker for anti-proliferation and growth arrest that occurs by prolonged activation of the ERK pathway (Nakano et al, 1997;Archer et al, 1998;Kim et al, 2002;Park et al, 2002;Eum et al, 2003). On the other hand, p21 Cip/WAF1 was not induced by the transient and weak ERK activation that is associated with proliferation (Sewing et al, 1997;Woods et al, 1997;Park et al, 2002).…”

Transduction of yeast cytosine deaminase mediated by HIV-1 Tat basic domain into tumor cells induces chemosensitivity to 5-fluorocytosine

“…For detection of yCD fusion proteins, the membrane was probed with rabbit anti-tagged histidine polyclonal antibody (Clontech, dilution 1:2,000), followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit antibody (Sigma, dilution 1:10,000). The bound antibodies were visualized by enhanced chemiluminescence according to the manufacturer's instruction (ECL; Amersham) (Kwon et al, 2000;Eum et al, 2003). For detection of cellular proteins, primary antibodies against PARP (mouse monoclonal antibody, Biomol), Bcl-2 (mouse monoclonal antibody, Santa Cruz), p53 (mouse monoclonal antibody, Neomarker), p21 (mouse monoclonal antibody, Pharmingen) and actin (mouse monoclonal antibody, Oncogene research products) were used (Draus et al, 2001).…”