Dexamethasone treatment improves morphological and hematological parameters in chronic experimental schistosomiasis

Pyrrho, Alexandre dos Santos; Lenzi, Henrique Leonel; Ramos, Juliene Antonio; Moura-Neto, Roberto; Cachem, Fabio Christiane O. F.; Silva, Célia Santos da; Takiya, Christina Maeda; Gattass, Cerli Rocha

doi:10.1007/s00436-004-1078-8

Cited by 13 publications

(9 citation statements)

References 28 publications

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“…For the evaluation of granuloma density, stained slides were observed using bright field microscopy and all granulomas containing central viable eggs were quantified. All evaluations were blind performed by two different observers [27]. The area of hepatic granuloma was determined in histological sections from 20 to 30 granulomas per animal, containing central viable eggs, randomly chosen.…”

Section: Methodsmentioning

confidence: 99%

Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant SpeciesMitracarpus frigiduson AdultSchistosoma mansoniWorms

Fabri

Aragão

Florêncio

et al. 2014

BioMed Research International

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The aims of this work were to evaluate the in vitro and in vivo schistosomicidal properties of the methanolic extract of the aerial parts of Mitracarpus frigidus (MFM) and to determine its HPLC profile. For the in vitro experiment, four pairs of adult worms, obtained from infected mice, were exposed to different concentrations of MFM (100 to 400 μg/mL) for 24 and 48 h and analyzed under an inverted microscope. For the in vivo experiment, mice were inoculated with cercariae and, 20 days after infection, MFM (100 and 300 mg/kg) was administered orally for the following 25 days. Mice were euthanized after 60 days. MFM showed in vitro schistosomicidal activity, exhibiting the opening of the gynaecophoral canal of some male schistosomes, the presence of contorted muscles, vesicles, and the darkening of the paired worms skin. In vivo experiments showed that MFM treatments significantly reduced total worm count, as praziquantel, showing a decrease in liver and spleen weight. Also, a significant reduction in granuloma density was observed. MFM treatment did not cause alterations in the liver function of either infected or noninfected mice. The HPLC chromatogram profile showed the presence of kaempferol-O-rutinoside, rutin, kaempferol, psychorubrin, and ursolic acid.

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Section: Methodsmentioning

confidence: 99%

Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant SpeciesMitracarpus frigiduson AdultSchistosoma mansoniWorms

Fabri

Aragão

Florêncio

et al. 2014

BioMed Research International

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“…In a previous paper, we showed that treatment with anti-inflammatory drugs such as dexamethasone can cause a decrease in granuloma size, a reduction in periovular fibrosis deposition and improved morphological and hematological parameters (Pyrrho et al 2004;Pyrrho et al 2002). Herein, we investigated the effects of silymarin on egg burden, granuloma sizes, and fibrosis.…”

Section: Introductionmentioning

confidence: 94%

Silymarin treatment reduces granuloma and hepatic fibrosis in experimental schistosomiasis

et al. 2010

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The schistosomiasis is a parasitic infection with relevant social impact and an important health problem in many countries around world. The pathology of this infection is characterized by a granulomatous reaction around parasite eggs and by hepatic fibrosis. Silymarin, a complex compound isolated from Silybum marianum (L.) Gaertner, have been described as hepatoprotective, antioxidant, antifibrotic, immunomodulator, and anti-neoplastic agent. Some of these capacities could potentially protect against pathology in schistosomiasis. Herein, we evaluated the effects of silymarin on parasite burden, granuloma sizes, and liver fibrosis, which are associated with severity and morbidity of this disease. BALB/c mice treated intraperitoneally with 10, 20, or 25 doses of silymarin (10 mg kg(-1)) suspended in carboxymethylcellulose were analyzed at 55 days post-infection. Silymarin (1) did not affect parasite oviposition capacity; (2) reduced granulomatous peri-ovular reaction in the liver, and (3) decreased hepatic fibrosis in this infection. Taken together, these data suggest that treatment with silymarin at acute phase of schistosomiasis may result in a mild course of murine schistosomiasis and can be a promising complementary treatment reverting sequelae of this infection.

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“…17 More recently, Doenhoff et al, 18 stated that aqueous extracts of Schistosoma mansoni eggs have been shown to have fibrinolytic activity inhibitable by a serine protease inhibitor. Last but not least, Pyrrho et al 19 investigated the possible use of immunomodulators as coadjuvants in the treatment of chronic Schistosoma mansoni infection. The authors evaluated the effect of dexamethasone in chronic experimental schistosomiasis.…”

Section: Discussionmentioning

confidence: 99%

“…These data suggest that treatment with dexamethasone results in a mild course of murine schistosomiasis and point to this drug as a promising agent to complement Schistosoma mansoni-specific treatment. 19 Conflict of interest: No conflict of interest to declare.…”

Section: Discussionmentioning

confidence: 99%

Prophylactic effect of the anti-inflammatory drug diclofenac in experimental schistosomiasis mansoni

Nessim

Mahmoud

2007

International Journal of Infectious Diseases

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Dexamethasone treatment improves morphological and hematological parameters in chronic experimental schistosomiasis

Cited by 13 publications

References 28 publications

Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant SpeciesMitracarpus frigiduson AdultSchistosoma mansoniWorms

Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant SpeciesMitracarpus frigiduson AdultSchistosoma mansoniWorms

Silymarin treatment reduces granuloma and hepatic fibrosis in experimental schistosomiasis

Prophylactic effect of the anti-inflammatory drug diclofenac in experimental schistosomiasis mansoni

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