Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells

Abstract

Dexpanthenol (D-panthenol) is a precursor of vitamin B5 (pantothenic acid) and is widely used for dietary supplements and topical applications. D-panthenol has long been used in hair care products for the purpose of anti-hair loss, its effects and the underlying mechanisms, however, were barely reported. In this study, the effects of D-panthenol on human hair follicle cells, including dermal papilla cells (hDPCs) and outer root sheath cells (hORSCs), were investigated. D-panthenol enhanced the cell viability, increasing the cellular proliferation marker Ki67 in cultured hDPCs. The markers for apoptosis (Caspase3/9) and cell senescence (p21/p16), reported to be expressed in aged or resting phase follicles, were significantly reduced by D-panthenol. Anagen-inducing factors (ALP; β-catenin; versican), which trigger or elongate the anagen phase, were stimulated by D-panthenol. On the other hand, D-panthenol reduced TGF-β1 expressions in both mRNA and protein levels. The expression of VEGF, which is important for peripheral blood vessel activation; was up-regulated by D-panthenol treatment. In cultured hORSCs, cell proliferation and viability were enhanced, while the mRNA expression of cell senescence markers (p21/p16) was significantly down-regulated. The expressions of both VEGF and its receptor (VEGFR) were up-regulated by D-panthenol. In conclusion, our data suggest that the hair growth stimulating activity of D-panthenol was exerted by increasing the cell viability, suppressing the apoptotic markers, and elongating the anagen phase in hair follicles.

Efficacy of intramuscular injections of biotin and dexpanthenol in the treatment of diffuse hair loss: A randomized, double‐blind controlled study comparing two brands

“…Shin et al, through animal studies discovered that dexpanthenol could have a beneficial impact on hair loss through proliferative and antiaging effects on follicular dermal papilla and outer root sheath cells. 3 In Kutulu et al's study, dexpanthenol was administered in patients with androgenic alopecia, and patient satisfaction improved significantly, 4 but the evidence is lacking regarding more objective impact of dexpanthenol on hair characteristics.…”

“…Shin et al, through animal studies discovered that dexpanthenol could have a beneficial impact on hair loss through proliferative and antiaging effects on follicular dermal papilla and outer root sheath cells 3 …”

“…• Any adverse drug reactions, including dermatitis, pruritus, and burning sensation were reported and graded as mild [1] moderate [2] and severe [3]. 6 All the objective and subjective assessments were performed before treatment, as well as 1 and 8 weeks after the last treatment session.…”

“…Vitamin B5 has a crucial role in acetyl‐COA production in fatty acids metabolism, much like pantothenic acid, or the precursor, dexpanthenol. However, unlike its well‐known anti‐inflammatory, hydrating and wound healing effects, its role in preventing or treating hair loss is controversial and not well studied 3,4,5 …”

Dekspantenol, Sıçanlarda LPS'nin Neden Olduğu Akut Akciğer Hasarında Artan VCAM-1 ve Kaspaz-3 Ekspresyonlarını Azaltarak İnflamasyonu ve Apoptozu İnhibe Eder

“…Dexpanthenol (Dex) is a stable alcoholic analogue of D-pantothenic acid (Vitamin B5), which is used especially in wounds, irritations and wrinkles, is easily accessible due to its cheapness, and is frequently found in local pharmacies (Heise et al 2012, Stettler et al 2017). In previous studies; it has been proven to be effective against oxidative stress on heart tissue (Kose et al 2020), against nephrotoxicity (Pınar et al 2020), hair strengthening (Shin et al 2021), and prevention of hepatotoxicity (Ucar et al 2018). In this study, it was aimed to prove the preservative effect of Dex in the case of ALI in rats.…”