Dextran: effect of process parameters on production, purification and molecular weight and recent applications

Vettori, Mary Helen Palmuti Braga; Blanco, Kate Cristina; Cortezi, Mariana; Lima, Cristian Jacques Bolner de; Contiero, Jonas

doi:10.7447/dc.2012.018

Cited by 24 publications

(23 citation statements)

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“…(109, 110) Additionally, the commercial production of dextran remains inefficient, presently limiting its widespread use as a biomaterial for bone tissue engineering. (111)…”

Section: Spheroids In Biomaterials For Cell-based Tissue Engineeringmentioning

confidence: 99%

Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

Gionet-Gonzales

Leach

2018

Biomed. Mater.

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There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection. Despite promising early results achieved by freely injecting spheroids into damaged tissues, growing evidence demonstrates the advantages of entrapping spheroids within a biomaterial prior to implantation. This review will highlight the basic characteristics and qualities of spheroids, describe the underlying principles for how biomaterials influence spheroid behavior, with an emphasis on hydrogels, and provide examples of synergistic approaches using spheroids and biomaterials for tissue engineering applications.

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“…(109, 110) Additionally, the commercial production of dextran remains inefficient, presently limiting its widespread use as a biomaterial for bone tissue engineering. (111)…”

Section: Spheroids In Biomaterials For Cell-based Tissue Engineeringmentioning

confidence: 99%

Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

Gionet-Gonzales

Leach

2018

Biomed. Mater.

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“…Among them, dextransucrases synthesize α-glucans comprising more than 50% of α-(1→6) osidic linkages called dextrans, which usually display a very High Molar Mass (HMM) over 10 6 g/mol. These HMM dextrans can be used as texturing, thickening and emulsifying agents, especially in food and cosmetic fields [2][3][4]. However, most of the applications concern fractions of 1, 10, 40 or 70.…”

Section: Introductionmentioning

confidence: 99%

Processivity of dextransucrases synthesizing very-high-molar-mass dextran is mediated by sugar-binding pockets in domain V

Claverie

Cioci

Vuillemin

et al. 2020

Journal of Biological Chemistry

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The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

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“…Polymers of bacterial origin that are obtained in an environmentally friendly manner are called “green polymers.” The production of these polymers is generally carried out using renewable carbon sources as raw materials [1]. These polymers have attracted interest from several sectors, including pharmaceutical, chemical, and food industries, owing to their beneficial biotechnological and pharmacological properties [2].…”

Section: Introductionmentioning

confidence: 99%

“…Dextran has been gaining considerable interest owing to its ability to form viscous solutions and gels in the aqueous medium even at low concentrations, stability in a wide pH and temperature range, non-ionic nature, and good stability during various industrial processes [2]. These properties have extended the applications of dextran in industries in the form of an emulsifier, thickener, suspending agent, gelling agent, stabilizer, binder, coagulant, lubricant, and protective colloid [5].…”

Section: Introductionmentioning

confidence: 99%

Gallic Acid-Dextran Conjugate: Green Synthesis of a Novel Antioxidant Molecule

et al. 2019

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A novel derivative of dextran, dextran–gallic acid (Dex–Gal), obtained from simple conjugation with gallic acid, was synthesized by an efficient free radical-mediated method. To verify the synthesis of Dex–Gal, 1H-nuclear magnetic resonance (1H-NMR), Fourier transform infrared (FTIR) spectrometry, and high-performance size-exclusion chromatography (HPSEC) were employed. The results revealed the conjugation of gallic acid with the 15.5 kDa dextran from Leuconostoc mesenteroides. Dex–Gal had a molecular weight of 11.2 kDa, indicating that the conjugation reaction was accompanied by a minor degradation of Dex–Gal. In addition, Dex–Gal contained 36.8 ± 1.4 mg gallic acid per gram dextran. These molecules were also evaluated as antioxidants using total antioxidant capacity (TAC), reducing power, ferric chelation, and superoxide radical-scavenging assays. Both polysaccharides had no ferric chelation activity. In addition, Dex–Gal was more efficient as an antioxidant agent in TAC (13 times) and was more efficient than dextran in superoxide radical-scavenging (60 times) and reducing power (90 times) assays. These data demonstrate that Dex–Gal is a natural-compound-based antioxidant with potential applications in the pharmaceutical, cosmetic, and food industries.

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Dextran: effect of process parameters on production, purification and molecular weight and recent applications

Cited by 24 publications

References 0 publications

Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin

Processivity of dextransucrases synthesizing very-high-molar-mass dextran is mediated by sugar-binding pockets in domain V

Gallic Acid-Dextran Conjugate: Green Synthesis of a Novel Antioxidant Molecule

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