Dextran Fluorescent Probes Containing Sulfadiazine and Rhodamine B Groups

Abstract

Fluorescent imaging has been expanded, as a non-invasive diagnostic modality for cancers, in recent years. Fluorescent probes in the near-infrared window can provide high sensitivity, resolution, and signal-to-noise ratio, without the use of ionizing radiation. Some fluorescent compounds with low molecular weight, such as rhodamine B (RhB) and indocyanine green (ICG), have been used in fluorescent imaging to improve imaging contrast and sensitivity; however, since these probes are excreted from the body quickly, they possess significant restrictions for imaging. To find a potential solution to this, this work investigated the synthesis and properties of novel macromolecular fluorescent compounds. Herein, water-soluble dextran fluorescent compounds (SD-Dextran-RhB) were prepared by the attachment of RhB and sulfadiazine (SD) derivatives to dextran carrier. These fluorescent compounds were then characterized through IR, 1H NMR, 13C NMR, UV, GPC, and other methods. Assays of their cellular uptake and cell cytotoxicity and fluorescent imaging were also performed. Through this study, it was found that SD-Dextran-RhB is sensitive to acidic conditions and possesses low cell cytotoxicities compared to normal 293 cells and HepG2 and HeLa tumor cells. Moreover, SD-Dextran-RhB demonstrated good fluorescent imaging in HepG2 and HeLa cells. Therefore, SD-Dextran-RhB is suitable to be potentially applied as a probe in the fluorescent imaging of tumors.

In Vivo Pharmacokinetic Study of Polygonatum cyrtonema Polysaccharide DPC1 after Oral and Intraperitoneal Administration

“…Fluorescence labeling of DPC1 was performed as previously described [ 48 ]. DPC1-RhB was obtained by esterification of the active carboxyl group of RhB with the hydroxyl group of DPC1.…”

A novel layered double hydroxide-based ternary nanocomposite for the effective photocatalytic degradation of rhodamine B

“…Therefore, a suitable treatment should be established before discharging dye effluents to the water stream. To date, various techniques have been documented for eliminating RhB from aqueous solutions, [1][2][3] including advanced oxidation, 5 adsorption, 6 ionizing radiation, 7 ion exchange, 8 among other methods. However, achieving the removal of RhB at low concentrations is a difficult and challenging task.…”

Graphene Oxide Nanostructures as Nanoplatforms for Delivering Natural Therapeutic Agents: Applications in Cancer Treatment, Bacterial Infections, and Bone Regeneration Medicine

“…NGO-NH2 (aminated), NGOpolyacrylamide functionalization (NGO-PAM), NGO-polyacrylic acid functionalization (NGO-PAA), NGO-pegylated functionalization (NGO-PEG), NGO-chitosan, and other modifications are noteworthy among these functionalizations (Figure 2). [33], polyacrylamide (PAM) [34], dextran [35], and polycaprolactone (PCL) [36]. Polyacrylic acid (PAA), APTES, dextran, and other chemical structures were obtained from chemspider.com, the website of Royal Society of Chemistry (http://www.chemspider.…”

“…Chemical structures of molecules frequently used for functionalization of GO-based materials. Chemical structures of chitosan[33], polyacrylamide (PAM)[34], dextran[35], and polycaprolactone (PCL)[36]. Polyacrylic acid (PAA), APTES, dextran, and other chemical structures were obtained from chemspider.com, the website of Royal Society of Chemistry (http://www.chemspider.…”