Dextran sulfate-resistant A/Puerto Rico/8/34 influenza virus is associated with the emergence of specific mutations in the neuraminidase glycoprotein

Yamada, Hiroshi; Nagao, Chioko; Haredy, Ahmad M.; Mori, Yasuko; Mizuguchi, Kenji; Yamanishi, Koichi; Okamoto, Shigefumi

doi:10.1016/j.antiviral.2014.09.002

Cited by 6 publications

(10 citation statements)

References 39 publications

(50 reference statements)

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“…We previously reported that the 430-loop of PR8 NA was the main target for DS (Yamada et al, 2014), which was similar pattern of suppressive influenza virus replication by D35. Here we found that the K435E mutation in the 430-loop of D35-sensitive PR8 NA changed it to D35-resistant.…”

Section: Discussionsupporting

confidence: 57%

“…We reported that DS suppressed the replication of influenza A viruses by electrically interacting with viral proteins through its sulfate containing negative charge (Yamada et al, 2014). The result raised a hypothesis that ODNs, as well as DS, may suppress the viral replication, because it is well known that the existence of PO bond results in electrically negative charge of ODNs.…”

Section: Introductionmentioning

confidence: 94%

“…A/ Puerto Rico/8/34 (H1N1) (PR8) and A/WSN/33 (H1N1) (WSN) were generated by plasmid-based reverse genetics (Neumann et al, 1999;Horimoto et al, 2007), and plasmids (pHH21) each containing a genomic segment of PR8 and WSN and four WSN protein expression plasmids encoding PB1, PB2, PA and NP were kindly provided by Dr. Y. Kawaoka (University of Wisconsin, USA). Reassorted viruses between PR8 and WSN and recombinant viruses of PR8 with mutated NA genes were described previously (Yamada et al, 2012(Yamada et al, , 2014.…”

Section: Cells and Virusesmentioning

confidence: 99%

“…We also reported that the critical amino acids of PR8 NA for DSsensitivity were basic amino acids, R430, K432 and K435, in the 430-loop which corresponds to amino acids from R430 to T439 ( Supplementary Fig. 1) (Yamada et al, 2014). We examined the plaque formation of a series of recombinant viruses in which NA had a single mutation found in DS-resistant PR8 viruses.…”

Section: K432 and K435 Of Na Are Responsible For The Inhibition Of VImentioning

confidence: 99%

“…Recently we reported that DS also suppressed the NA activity and release from infected cells at the late stage of the replication, resulting in the suppressive viral replication (Yamada et al, 2012). Since DS is negatively charged, the change in the viral surface from a positive to negative charge by adding DS may play a role in the suppression of viral replication (Moulard et al, 2000;Yamada et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Toll-like receptor 9 ligand D-type oligodeoxynucleotide D35 as a broad inhibitor for influenza A virus replication that is associated with suppression of neuraminidase activity

et al. 2016

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The most effective drugs available to treat influenza are neuraminidase (NA) inhibitors, which provide important additional measures for the control of influenza virus infections. However, since the emergence of NA inhibitor-resistant viruses may compromise the clinical utility of this class of anti-influenza agents, it is very important to develop new anti-influenza agents which target a different region in NA responsible for its sensitivity from that for NA inhibitors and could be used to treat NA inhibitors-resistant isolates. The oligodeoxynucleotide D35, multimerized and aggregated, suppressed replication of influenza A viruses except A/WSN/33 (WSN). The suppressive viral replication by D35 depended on G-terad and multimer formation. The range of the suppressive viral replication at the late stage, including virus assembly and release from infected cells, was much larger than that at the initial stage, viral attachment and entry. D35 suppressed NA activity of influenza A viruses. Furthermore, replacing the NA gene of A/Puerto Rico/8/34 (PR8), in which viral replication was inhibited by D35 at the late stage, with the NA gene from WSN, in which viral replication was not inhibited, eliminated the D35-dependent suppression. D35 showed an additive anti-influenza effect with oseltamivir. It was also effective in vivo. These results suggest that the influenza virus NA mainly contributes to the D35-suppressible virus release from infected cells at the late stage. In addition, because administration of D35 into the virus-infected mice suppressed viral replication and weight loss, clinical application of D35 could be considered.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 94%

Section: Cells and Virusesmentioning

confidence: 99%

Section: K432 and K435 Of Na Are Responsible For The Inhibition Of VImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toll-like receptor 9 ligand D-type oligodeoxynucleotide D35 as a broad inhibitor for influenza A virus replication that is associated with suppression of neuraminidase activity

et al. 2016

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From Bulk to Nanoparticles: An Overview of Antiviral Materials, Its Mechanisms, and Applications

Rodrigues

Campo

Arns

et al. 2021

Part & Part Syst Charact

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Infectious diseases caused by viruses are a global health concern and have become prominent in light of the recent COVID‐19 pandemic. Considering the limitations of drugs and prophylactic methods used in current medicine, antiviral materials are a useful strategy in preventing the spread of viruses and enhancing treatment efficiency. Thus, this review highlights the state‐of‐the‐art antiviral materials, describes the scientific landscape of the primary antiviral materials used based on bibliometric analysis, presents their mechanisms of action, and discusses their clinical applications. The mechanisms of action underlying the broad‐spectrum antiviral properties of metals, ceramics, polymers, and composites are also discussed. Polyanions, polycations, oxides, and metal‐based materials, from bulk to nanoparticles, have good potential in antiviral applications that may help prepare the world for future viral breakouts.

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Emerging insights into the structure-activity relationship of water-soluble polysaccharides in antiviral therapy

Sharma,

Shahid,

Banerjee

et al. 2024

European Journal of Medicinal Chemistry Reports

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Dextran sulfate-resistant A/Puerto Rico/8/34 influenza virus is associated with the emergence of specific mutations in the neuraminidase glycoprotein

Cited by 6 publications

References 39 publications

Toll-like receptor 9 ligand D-type oligodeoxynucleotide D35 as a broad inhibitor for influenza A virus replication that is associated with suppression of neuraminidase activity

Toll-like receptor 9 ligand D-type oligodeoxynucleotide D35 as a broad inhibitor for influenza A virus replication that is associated with suppression of neuraminidase activity

From Bulk to Nanoparticles: An Overview of Antiviral Materials, Its Mechanisms, and Applications

Emerging insights into the structure-activity relationship of water-soluble polysaccharides in antiviral therapy

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