Dextran vitrification media prevents mucin coat and zona pellucida damage in rabbit embryo

Viudes-de-Castro, M.P.; Cortell, C.; Vicente, J.S.

doi:10.1016/j.theriogenology.2010.06.034

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…In several species as human or rabbits, cracked zona pellucida or mucin coat respectively is enough to reduce drastically in vivo development of cryopreserved embryos (Kasai et al, 1996). However, Viudes et al (2010) found that in vitro developmental ability of undamaged embryos was not affected by superovulation treatment nor vitrification media in the current experiment. However, further studies of the in vivo viability of cryopreservedsuperovulated rabbit embryos with dextran addition to the vitrification media must be done.…”

Section: Introductioncontrasting

confidence: 63%

Effect of Superovulation on Ovulatory Response, Embryo Recovery and Embryo Measurements in Baladi Red Rabbit Does

Dorra¹,

Sherif²,

Shamiah³

et al. 2013

Journal of Animal and Poultry Production

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Total of 24 rabbit does (5-7 mo of age, 3-4 kg LBW and 1-2 parities were used to study the effect of superovulation by PMSG on ovarian characteristics, quality and measurements of embryos at different stages (pronucli, morula and blastocyst) of Baladi Red (BR) rabbit does. Also, 3 fertile BR bucks were used for natural mating. All does and bucks were kept under the same conditions of feeding and management. Does in the 1 st group (n=12) were injected with 20 mg GnRH/doe (0.2 ml Receptal) immediately after natural mating (control, G1), while does in the 2 nd group (n=12) were superovulated by injection of 40 IU/kg LBW from PMSG (Foligon), followed by 0. 2 ml receptal immediately after natural mating (treatment, G2). Does in G1 and G2 were sub-divided into 3 sub-groups, 4 does in each. Ovarian characteristics were determined and embryos were recovered by flushing from each treated doe in each sub-group slaughtered after 40-46 h of mating for collection of embryos at pronucli stage (1-16 cell embryos), after 60-64 h of mating for embryo collection at morula stage and after 70-72 h of mating for those at blastocyst stage. Embryos were recovered from each uterine horn and oviduct per doe by flushing and morphologically measured for thickness of mucin coat (MC), zona pellucida (ZP) and interzonal (IZ), as well as total diameter of embryos (TDE) with or without MC at different stages. Results show that average ovarian weight (right and left) and ovarian weight relative to LBW were higher (P<0.05) in G2 than in G1 (0.26 and 0.22; 0.15 vs. 0.33 and 0.27 g/doe; 0.19 g/kg LBW, respectively). Ovulatory response in terms of average number of normal follicles (large and small), hemorrhagic follicles and total follicles and average number of corpora lutea (CLs) were greater (P<0.05) in G2 than in G1

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Section: Introductioncontrasting

confidence: 63%

Effect of Superovulation on Ovulatory Response, Embryo Recovery and Embryo Measurements in Baladi Red Rabbit Does

Dorra¹,

Sherif²,

Shamiah³

et al. 2013

Journal of Animal and Poultry Production

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show abstract

“…However, it fails significantly during in vivo development at term, where intact mucin coat is essential for implantation. Concordantly, there is known that alterations in the mucin coat diminish in vivo development drastically, being the primary factor of embryonic loss in cryopreserved rabbit embryos [18,23,26]. Typically, asynchronous oviductal embryo transfer allows to restore defects in the mucin coat after in vitro manipulation [7], but extensive disturbances on the embryo coverage could be irreversible.…”

Section: Discussionmentioning

confidence: 99%

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

et al. 2020

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During the last decades, many techniques have been developed to reduce sample volume and improve cooling and warming rates during embryo vitrification. The vast majority are based on the "minimum drop size" concept, in which the vitrification solution around embryos is reduced by aspiration, leaving a tiny part of volume surrounding embryos.However, novel cryodevices were aimed to remove the entire vitrification solution. This study was designed to compare the "minimum drop size" technique using Cryotop® with the nylon mesh as cryodevice on rabbit morula embryos. The outcomes assessed were the in vitro development rates (experiment 1) and the offspring rates at birth (experiment 2).Embryos were vitrified in a two-step procedure; equilibrium (10% EG + 10% Me2SO) for 2 min and vitrification (20% EG + 20% Me2SO) for 1 minutes. In experiment 1, embryos (n= 323) were warmed and subsequently in vitro cultured for 48 h to assess the embryo developmental capability to reach the hatching-hatched blastocyst stage. In experiment 2, embryos were transferred using the laparoscopic technique (n=369) to assess the offspring rate at birth. In this context, rates of in vitro embryo development were similar between vitrified groups (0.73±0.042% and 0.66±0.047% for Cryotop® and nylon mesh device, respectively), but lower than in the fresh group (0.97±0.016%, p<0.05). In experiment 2, there were no significant differences in survival rates (offspring born/total embryos transferred) among the Cryotop® device group and fresh group (0.41±0.049% and 0.49±0.050%, respectively). But significantly lower value was obtained in the nylon mesh device group (0.18±0.030%). These results indicate that nylon mesh is not suitable as cryodevice for rabbit morula vitrification, remaining those using the "minimum drop size" methodology as the best option.

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“…The present findings are consistent with those of Kuliková et al (2014) where the addition of a large molecular weight polymer (Ficoll 70) to the cryopreservation medium containing sucrose and Me2SO that led to a marked increase in the number of acrosome intact sperm. The possibility that dextran inclusion in rabbit semen extenders may contribute to protection of membranes is also supported by embryo cryopreservation research findings, in which a reduction in deleterious effects on embryonic membranes occurred when large molecular weight polymers were used as an extracellular cryoprotectant (Carroll et al, 1993;Shaw et al, 1997;Nowshari and Brem, 2000;Checura and Seidel, 2007;Viudes de Castro et al, 2010). Furthermore, considering that only sperm maintaining an intact acrosome have the capacity for fertilization of an oocyte, this seminal variable could be very important in fertility outcomes when there is use of frozen thawed semen for AI.…”

Section: Discussionmentioning

confidence: 92%

“…It has been reported that aqueous solutions of polymers reduce the crystallization temperatures and the equilibrium melting points and they can easily be supercooled (Kimizuka et al, 2008). In a previous study, the addition of 10% dextran to vitrification medium resulted in an improved preservation of the essential membranes of rabbit embryos at the 3-day developmental stage (Viudes de Castro et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen–thawed rabbit sperm quality variables and reproductive performance

Viudes-de-Castro

Talaván

Vicente

2021

Animal Reproduction Science

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Dextran vitrification media prevents mucin coat and zona pellucida damage in rabbit embryo

Cited by 8 publications

References 34 publications

Effect of Superovulation on Ovulatory Response, Embryo Recovery and Embryo Measurements in Baladi Red Rabbit Does

Effect of Superovulation on Ovulatory Response, Embryo Recovery and Embryo Measurements in Baladi Red Rabbit Does

The harmful effect of removing the extracellular vitrification medium during embryo cryopreservation using a nylon mesh device in rabbit

Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen–thawed rabbit sperm quality variables and reproductive performance

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