Dezenfektan Biyositler ve Proteaz ve Amilaz Enzimlerinin Biyofilmlerdeki Bakteriler Üzerine Aktivitesi

Kukhtyn, Mykola; Kozhyn, Vladislav; Horiuk, V. V.; Malimon, Z. V.; Horiuk, Yulia; Yashchuk, Tetyana; Kernychnyi, S. P.

doi:10.9775/kvfd.2021.25770

Cited by 2 publications

(2 citation statements)

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“…Попередні лабораторні дослідження (Kukhtyn et al, 2021;Kozhyn et al, 2021) показали, що створений деззасіб є високоактивним щодо бактерій у біоплівках та мікрофлори навіть за значного органічного забруднення. Тому проведення подальших лабораторних досліджень з визначення фізико-хімічних показників, які всебічно характеризують його дію, є обов'язковим на етапі оцінки в умовах invitro.…”

Section: вступunclassified

“…Саме наявність ензимів у складі деззасобу дозволяє руйнувати захисний пептидносахаридний матрикс мікробної біоплівки на поверхнях, що обробляються, і тим самим поліпшувати контакт дезінфектанта з мікробними клітинами. Адже успішна боротьба з мікроорганізмами, які перебувають у біоплівках, можлива за умови застосування дезінфікуючих засобів, які руйнують екзополісахаридний матрикс (Kukhtyn et al, 2021). Дослідження з визначення протеолітичної активності деззасобу "Ензидез" щодо білків молока виявило, що дезінфіктант розщеплював білки за концентрації розчинів 0,5-1,0 % за температури + 20 °С на 41,3-43,1 % відповідно протягом 15 хв експозиції.…”

Section: обговоренняunclassified

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Evaluation of disinfectant “Enzidez” according to physical and chemical parameters

et al. 2022

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The dependence of the national economy on disinfectants is constantly growing through prevention strategies and the development of resistance in microorganisms. Therefore, new disinfectants with different mechanisms of biocidal activity for a wide range of pathogens are appearing on the market. The aim of work has been to determine the physical and chemical parameters of the created disinfectant “Enzidez” active on biofilm forms of bacteria and the possible organic load. The magnitude of the surface tension of disinfectant “Enzidez” at different concentrations of solutions has been determined using a device with a Traube stalagmometer. The wettability of solutions has been determined by the method of measuring the wetting angle with a catheter. Washing properties and stability of foam by the method of washing animal fats, followed by application of indicator liquid. Proteolytic activity has been determined using milk and the effect of Enzidez solutions of different concentrations on it, followed by measurement of the optical density of the solutions spectrophotometrically at a wavelength of 600 nm. It is established that the disinfectant with enzymes – “Enzidez” for disinfection in veterinary clinics for pre-sterilization disinfection and treatment of products and instruments of surgical purpose (plastic, rubber, glass, stainless steel) and objects of veterinary supervision (tables, utensils, walls, floors, doors, windows, etc.) with organic load is well soluble in water and has a pH of 0.25–1.0 % solutions in the range of 8.2–8.0 units. At a solution temperature of +20 ± 1 °С it has had a surface tension not higher than 37.63 ± 0.50 mN/m and a wetting angle of 69.5 ± 0.7 degree. The disinfectant has provided an excellent detergent effect only at solutions with a concentration of 1.0 % and above. When assessing the corrosive action of Enzidez in the concentration of solutions from 0.25 to 1.0 %, the amount of corrosion on stainless steel has been hundreds of times lower than the allowable regulatory limit. The impact on galvanized steel is slightly higher than that of stainless steel, but also several tens below the norm. A study to determine the proteolytic activity of the disinfectant “Enzidez” on milk proteins has revealed that the disinfectant broke down proteins at a concentration of solutions 0.5–1.0 % at a temperature of + 20 °C at 41.3–43.1 %, respectively, for 15 min of exposure. However, increasing the temperature of solutions to +60 °C and exposure for 30 min has provided an increase in proteolytic activity up to 70 %. Thus, disinfectant “Enzidez” is a highly active disinfectant for disinfecting surfaces even in the case of organic contamination.

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Evaluation of disinfectant “Enzidez” according to physical and chemical parameters

et al. 2022

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The influence of different methods of decontamination of microbial biofilms formed on eggshells

Kukhtyn,

Sverhun,

Horiuk

et al. 2024

Potr. S. J. F. Sci.

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According to "food legislation" requirements, all eggs entering the production of egg products must be disinfected. Therefore, developing technologies for decontaminating chicken eggs before use for food purposes is a promising work direction in chicken egg production and storage. This research aimed to identify the microbiota of chicken eggs with varying degrees of shell contamination and determine the influence of different methods of decontaminating microbial biofilms formed on eggshells. It was set up that the quantitative content of microorganisms on the surface of chicken eggs ranged from 103 CFU to 106 CFU/ml of washing and depended on the contamination of the shell with droppings. Lactobacillus spp., Bacillus spp., Corynebacterium, Staphylococcus were among the genera of bacteria that prevailed on the clean chicken shell, which were isolated in 30-50% of cases, and gram-negative microbiota was practically absent. The constant release of gram-positive bacteria is noted on the contaminated eggshell, and the frequency of identification of gram-negative microbiota of the Enterobacteriaceae genus and non-fermenting genera Pseudomonas and Psychrobacter increases. That is, the microbial scape of the microbiota of the chicken shell depends on its cleanliness, and the presence of a dirty surface increases the frequency of allocation of the resident microflora of the gastrointestinal tract. It was found that the working solution of the disinfectant Vircon S destroyed planktonic bacteria applied to the eggshell in an average of 2 minutes of exposure, stabilised water ozone for 1 minute, gaseous ozone for 3 minutes, and the action of ultraviolet rays with a length of 253.7 nm for 25-30 min. At the same time, using these disinfection methods on bacteria formed in a biofilm on the eggshell did not cause a bactericidal action during this time. To significantly reduce bacteria in the biofilm using these methods, it is necessary to increase the exposure time of the biocide by 2-3 times. Therefore, the complex structure of the eggshell and the multi-layered matrix of biofilms provide better protection for bacteria against the influence of the investigated disinfection methods.

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Dezenfektan Biyositler ve Proteaz ve Amilaz Enzimlerinin Biyofilmlerdeki Bakteriler Üzerine Aktivitesi

Cited by 2 publications

References 0 publications

Evaluation of disinfectant “Enzidez” according to physical and chemical parameters

Evaluation of disinfectant “Enzidez” according to physical and chemical parameters

The influence of different methods of decontamination of microbial biofilms formed on eggshells

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