DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130

Nardi, Francesca; Franco, Omar E.; Fitchev, Philip; Morales, Alejandro; Vickman, Renee E.; Hayward, Simon W.; Crawford, Susan E.

doi:10.1038/s41598-019-39537-z

Cited by 41 publications

(33 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunofluorescence (IF) staining was performed as described previously 46,47 . Immunohistochemistry and semiquantitative scoring techniques were performed as described previously 48 .…”

Section: Methodsmentioning

confidence: 99%

ELTD1 facilitates glioma proliferation, migration and invasion by activating JAK/STAT3/HIF-1α signaling axis

Shen

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The upregulation of ELTD1 ([epidermal growth factor (EGF), latrophilin and seven transmembrane domain-containing 1] on chromosome 1) in tumor cells has been reported in several types of cancer and correlates with poor cancer prognosis. However, the role of ELTD1 in glioma progression remains unknown. In this study, we examined ELTD1 expression levels in human glioma cell lines and in sixteen human gliomas of different grades. The molecular effects of ELTD1 in glioma cells were measured using quantitative polymerase chain reaction (qRT-PCR), Western blotting, Cell proliferation assays, Matrigel migration and invasion assays and brain orthotopic xenografts. We found that high expression levels of ELTD1 were positively associated with cancer progression and poor prognosis in human glioma. Mechanistically, ELTD1 activated the JAK/STAT3/HIF-1α signaling axis and p-STAT3 bound with HIF-1α. Taken together, our data provide a plausible mechanism for ELTD1-modulated glioma progression and suggest that ELTD1 may represent a potential therapeutic target in the prevention and therapy of glioma.

show abstract

“…Immunofluorescence (IF) staining was performed as described previously 46,47 . Immunohistochemistry and semiquantitative scoring techniques were performed as described previously 48 .…”

Section: Methodsmentioning

confidence: 99%

ELTD1 facilitates glioma proliferation, migration and invasion by activating JAK/STAT3/HIF-1α signaling axis

Shen

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…We recently showed that pharmacological inhibition of DGAT1 in breast and prostate cancer cells blunted TG synthesis and influenced cell viability [ 3 , 4 ]. Likewise, knockdown of DGAT1 reduced lipid droplet number and cell proliferation and invasion of prostate cancer cells [ 135 , 247 ] and glioblastoma [ 246 ]. However, the protein levels of DGAT2 are reduced in HCC, and overexpression of DGAT2 inhibits cell proliferation and colony formation in vitro and tumor formation in vivo [ 248 ].…”

Section: Tumor Fatty Acid Metabolism Pathways and Their Role In Cancementioning

confidence: 99%

The diversity and breadth of cancer cell fatty acid metabolism

2021

View full text Add to dashboard Cite

Tumor cellular metabolism exhibits distinguishing features that collectively enhance biomass synthesis while maintaining redox balance and cellular homeostasis. These attributes reflect the complex interactions between cell-intrinsic factors such as genomic-transcriptomic regulation and cell-extrinsic influences, including growth factor and nutrient availability. Alongside glucose and amino acid metabolism, fatty acid metabolism supports tumorigenesis and disease progression through a range of processes including membrane biosynthesis, energy storage and production, and generation of signaling intermediates. Here, we highlight the complexity of cellular fatty acid metabolism in cancer, the various inputs and outputs of the intracellular free fatty acid pool, and the numerous ways that these pathways influence disease behavior.

show abstract

“…8-9). DGAT1 encodes a key enzyme in lipogenesis, which was found increased in prostate cancer cells and could facilitate cell proliferation and migration 34 . DGAT1 gene expression was increased in both SRCCs and STADs (TCGA cohort) than non-malignant pairs, suggesting its increased expression in stomach adenocarcinoma universally.…”

Section: Discussionmentioning

confidence: 99%

Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method

Shu

et al. 2020

Commun Biol

View full text Add to dashboard Cite

Human genes form a large variety of isoforms after transcription, encoding distinct transcripts to exert different functions. Single-molecule RNA sequencing facilitates accurate identification of the isoforms by extending nucleotide read length significantly. However, the gene or isoform diversity is lowly represented by the mRNA molecules captured by singlemolecule RNA sequencing. Here, we show that a cDNA normalization procedure before the library preparation for PacBio RS II sequencing captures 3.2-6.0 fold more full-length highquality isoform species for different human samples, as compared to the non-normalized capture procedure. Many lowly expressed, functionally important isoforms can be detected. In addition, normalized PacBio RNA sequencing also resolves more allele-specific haplotype transcripts. Finally, we apply the cDNA normalization based long-read RNA sequencing method to profile the transcriptome of human gastric signet-ring cell carcinomas, identify new cancer-specific transcriptome signatures, and thus, bring out the utility of the improved protocols in gene expression studies.

show abstract

DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130

Cited by 41 publications

References 55 publications

ELTD1 facilitates glioma proliferation, migration and invasion by activating JAK/STAT3/HIF-1α signaling axis

ELTD1 facilitates glioma proliferation, migration and invasion by activating JAK/STAT3/HIF-1α signaling axis

The diversity and breadth of cancer cell fatty acid metabolism

Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method

Contact Info

Product

Resources

About