DGGE analysis of buffalo manure eubacteria for hydrogen production: effect of pH, temperature and pretreatments

Carillo, Petronia; Carotenuto, Claudia; Cristofaro, F. Di; Kafantaris, Ioannis; Lubritto, C.; Minale, Mario; Morrone, Biagio; Papa, Stefania; Woodrow, Pasqualina

doi:10.1007/s11033-012-1894-3

Cited by 17 publications

(9 citation statements)

References 30 publications

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“…ShannoneWiener diversity index and Simpson diversity index both performed a decreasing trend with the increase of fermentation temperature (except 30 C for activated sludge), indicating that the higher the fermentation temperature, the lower the microbial community diversity. The trend was also reported by previous studies [22,45,46], and the reason might be possible susceptibility of many microbial species towards high temperatures. Pielou evenness index also showed similar variations with the other two diversity indexes.…”

Section: Microbial Community Structuresupporting

confidence: 87%

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

Zhang

Ren

Wang

2015

International Journal of Hydrogen Energy

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Temperature Seed sludgeCorn stover hydrolyzate Denaturing gradient gel electropho- resis (DGGE)Microbial diversity analysis a b s t r a c tThe temperature effect on fermentative hydrogen (H 2 ) production from corn stover hydrolyzate was investigated under mesophilic (37 and 30 C), thermophilic (55 C), and extreme thermophilic (70 C) conditions by using two typical seed sludges (activated sludge and anaerobic granular sludge). Among various temperatures, the fermentation at 55 C reached the optimal H 2 production with the values of 6.08 mmol-H 2 /g-utilized sugar for activated sludge and 7.74 mmol-H 2 /g-utilized sugar for anaerobic granular sludge, respectively. For the two seed sludges, the effectiveness of fermentation temperature on H 2 production both followed the order as 55 C > 70 C > 37 C z 30 C. The soluble metabolites composition at 55 C showed the highest acetate and butyrate concentrations, as well as the minimum ethanol production, coinciding with better H 2 -producing performances in these cases. Microbial community analysis indicated that microbial community diversity significantly decreased with increased fermentation temperature. Facultative anaerobes, such as Enterobacter spp., Klebsiella spp., and Citrobacter spp., were dominant in microbial community of the two seed sludges. As efficient H 2 producers, Bacillus sp. AB5283 in activated sludge and Thermoanaerobacterium sp. PO-2009 in anaerobic granular sludge might be mainly responsible for high H 2 yields under thermophilic condition.

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Section: Microbial Community Structuresupporting

confidence: 87%

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

Zhang

Ren

Wang

2015

International Journal of Hydrogen Energy

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“…The metabolic pathway can be schematically divided into four main phases: hydrolysis, acidogenesis, acetogenesis and methanogenesis and each step is performed by different consortia of microorganisms [7,8]. The microorganisms producing methane, during the last digestion phase, are called methanogens and they can be classified according to their optimal pH range [9]; in particular, to maximize the CH4 yield, pH typically varies from 6.5 to 8.2, with optimal values of 7.0-7.2 [10].…”

Section: Introductionmentioning

confidence: 99%

Biogas Production from Anaerobic Digestion of Manure at Different Operative Conditions

Carotenuto¹,

Guarino²,

Minale³

et al. 2016

IJHT

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Anaerobic digestion is an established technology to simultaneously produce biogas and treat different kinds of wastes. This work deals with water buffalo manure digested under mesophilic (37°C) and thermophilic (55°C) conditions with initial pH varying from 6.0 to 8.7. The digestion is investigated following the evolution of the biogas composition in time, in terms of the volume fraction of CH4, CO2 and their ratio. The growths of the biogas products are interpolated with a Gompertz equation and in all the investigated cases the methane productivity is above 63% with a CH4/CO2 ratio rather high and equal to about 2. In mesophilic conditions, an initial pH of 7.0 must be preferred to optimize the methane yield and to minimize the time required to attain it. The thermophilic conditions resulted very promising since they almost halved the time required to reach the final yield. The speed up of the process at 55°C, in our case, resulted to be due to the increase of the digestion kinetic more than to the selection of a new metabolic pathway.

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“…Hydrogen is an intermediate product of the anaerobic fermentation and thus, if the digestion process is accurately designed and controlled the H2 yield can be maximized so to produce an innovative biogas mainly made of CH4, H2 and CO2 [44]. Water buffalo manure naturally contains the eubacteria responsible of the hydrogen production [43] and thus it can be considered as a very promising substrate to produce the innovative biogas [45]. The authors then assumed water buffalo manure as a reference organic matter from which, as experimentally demonstrated, an innovative biogas with an H2/CO2 molar ratio of about 1 and a maximum H2/CH4 molar ratio of 0.25 can be produced [46].…”

Section: Biogasmentioning

confidence: 99%

EGR Strategy for NOx Emission Reduction in a CAI Engine Fuelled with Innovative Biogas

Mariani¹,

Unich²,

Minale³

2019

IJES

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Gaseous fuel utilization reduces pollutant emissions and makes thermal energetic systems less dependent on oil. Their carbon content is low, which means low CO2 emissions, and a lower cost with respect to conventional fuels. If gaseous fuels have a non-fossil origin, their adoption would have a beneficial impact on global CO2 balance and on the dependence of the energy sector from fossil fuels. Biogas is produced from the anaerobic digestion of organic materials, with the composition depending on the feedstock and on the production processes. The use of biogas in internal combustion engines (ICE) is very attractive. However, the presence in the biogas of an inert gas like CO2 has adverse effects on combustion, reducing combustion speed, narrowing flammability limits with harmful effects on combustion stability. A possible way for improving combustion performance of biogas is hydrogen addition. Innovative anaerobic digestion processes, which maximize the H2 yield, can end up with biogases made of CH4, CO2 and H2. The biogas, generally adopted as fuel in positive ignition ICEs, is also suitable for Controlled Auto Ignition (CAI) engines. Aim of this work is to investigate the biogas combustion in CAI ICE by means of numerical simulations. The effect of fuel composition and EGR on engine performance and exhaust emissions was evaluated. Authors compared conventional and innovative biogases composed by CH4, CO2 and H2 of different compositions. CAI systems are controlled with varying intake gas conditions: air-fuel ratio, boost pressure and charge temperature. The temperature depends on recycled exhaust gas content, which has an impact on the combustion process. Authors investigated the effect that EGR has on the in-cylinder gas temperature and reaction mechanism, focusing on NOx emission formations.

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DGGE analysis of buffalo manure eubacteria for hydrogen production: effect of pH, temperature and pretreatments

Cited by 17 publications

References 30 publications

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature

Biogas Production from Anaerobic Digestion of Manure at Different Operative Conditions

EGR Strategy for NOx Emission Reduction in a CAI Engine Fuelled with Innovative Biogas

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