DgMYB2 improves cold resistance in chrysanthemum by directly targeting <i>DgGPX1</i>

Yang, Xiaohan; Luo, Yunchen; Bai, Huiru; X, Li; Tang, Si; Liao, Xiaoqin; Zhang, Lei; Liu, Qinglin

doi:10.1093/hr/uhab028

Cited by 27 publications

(15 citation statements)

References 29 publications

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“…It mainly regulates the expression of related genes through the interaction between specific amino acids and target genes. NtbHLH123 transcription factor in tobacco enhances tolerance to cold stress through regulatory pathways and reactive oxygen homeostasis [ 65 ]. MYB and bHLH families are believed to regulate plant secondary metabolism and stress responses according to combinatorial interactions of MYB and bHLH proteins or multi-protein complexes formed of different subgroups of bHLH proteins [ 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

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Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Dong

Liu

et al. 2022

BMC Plant Biol

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Background Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severely damaged under extreme cold conditions. However, studies about the transcriptional and metabolic mechanisms underlying cold stresses in wheat are limited so far. Results In this study, 14,466 differentially expressed genes (DEGs) were obtained between wild-type and cold-sensitive mutants, of which 5278 DEGs were acquired after cold treatment. 88 differential accumulated metabolites (DAMs) were detected, including P-coumaroyl putrescine of alkaloids, D-proline betaine of mino acids and derivativ, Chlorogenic acid of the Phenolic acids. The comprehensive analysis of metabolomics and transcriptome showed that the cold resistance of wheat was closely related to 13 metabolites and 14 key enzymes in the flavonol biosynthesis pathway. The 7 enhanced energy metabolites and 8 up-regulation key enzymes were also compactly involved in the sucrose and amino acid biosynthesis pathway. Moreover, quantitative real-time PCR (qRT-PCR) revealed that twelve key genes were differentially expressed under cold, indicating that candidate genes POD, Tacr7, UGTs, and GSTU6 which were related to cold resistance of wheat. Conclusions In this study, we obtained the differentially expressed genes and differential accumulated metabolites in wheat under cold stress. Using the DEGs and DAMs, we plotted regulatory pathway maps of the flavonol biosynthesis pathway, sucrose and amino acid biosynthesis pathway related to cold resistance of wheat. It was found that candidate genes POD, Tacr7, UGTs and GSTU6 are related to cold resistance of wheat. This study provided valuable molecular information and new genetic engineering clues for the further study on plant resistance to cold stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Dong

Liu

et al. 2022

BMC Plant Biol

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“…Overexpression of DgTIL1 improved the tolerance of chrysanthemum to low-temperature stress, and the lysine crotonylation of DgTIL1 prevented the degradation of the non-specific lipid transfer protein DgnsLTP in chrysanthemum, thereby promoting the activity of peroxidase (POD) and reactive oxygen species (ROS) scavenging and therefore improving the tolerance of chrysanthemum to low-temperature stress [ 15 ]. DgMYB2 promotes increased glutathione peroxidase (GPX) activity by regulating the expression level of DgGPX1, reduces the accumulation of ROS in chrysanthemum, and improves the tolerance of chrysanthemum to low-temperature stress [ 16 ]. Decrotonylation of DgGPX1 at lysine K220 can promote the enhancement of GPX activity, thereby reducing the accumulation of ROS in chrysanthemum and improving cold resistance [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

DgbZIP3 interacts with DgbZIP2 to increase the expression ofDgPODfor cold stress tolerance in chrysanthemum

Bai

Liao

et al. 2022

Horticulture Research

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The bZIP transcription factor plays a very important role in abiotic stresses for instance drought, salt, and low-temperature stress, but the mechanism of action at low temperature is still unclear. In this study, overexpression of DgbZIP3 led to increased whereas antisense suppression of DgbZIP3 resulted in decreased tolerance of chrysanthemum (Chrysanthemum morifolium Ramat.) to cold stress. Electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP), luciferase complementary imaging analysis (LCI), and dual luciferase reporter gene detection (DLA) experiments indicated that DgbZIP3 directly bound to the promoter of DgPOD and activated it expression. DgbZIP2 was identified as a DgbZIP3-interacting protein using yeast two-hybrid (Y2H), co-immunoprecipitation (Co-IP), LCI, and bimolecular fluorescence complementation (BIFC) assays. Overexpression of DgbZIP2 led to increased whereas antisense suppression of DgbZIP2 resulted in decreased tolerance of chrysanthemum to cold stress. A ChIP–qPCR experiment showed that DgbZIP2 was highly enriched in the promoter of DgPOD, while DLA, EMSA, and LCI experiments further showed that DgbZIP2 could not directly regulate the expression of DgPOD. The above results show that DgbZIP3 interacts with DgbZIP2 to regulate the expression of DgPOD to promote an increase in peroxidase activity, thereby regulating the balance of ROS and improving the tolerance of chrysanthemum to low-temperature stress.

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“…As the largest transcription factor family, many reports have showed the role of MYB in plant cold resistance such as: MdMYB88/124 [55], MdMYB23 [56], and MdMYB308L [57] in apple; PbrMYB5 [58] in pear; and DgMYB2 [59] in Chrysanthemum. Studies of describing the role of MYB transcription factors in grape cold resistance have been reported [7].…”

Section: Discussionmentioning

confidence: 99%

Discovery of cold-resistance genes in Vitis amurensis using bud-based quantitative trait locus mapping and RNA-seq

Zhao

Lin

et al. 2022

BMC Genomics

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Background In cold regions, low temperature is the main limiting factor affecting grape production. As an important breeding resource, V. amurensis Rupr. has played a crucial role in the discovery of genes which confer cold resistance in grapes. Thus far, many cold-resistance genes have been reported based on the study of V. amurensis. In order to identify more candidate genes related to cold resistance in V. amurensis, QTL mapping and RNA-seq was conducted based on the hybrid population and different cold-resistance cultivars in this study. Results In this study, highly cold-resistant grape cultivar ‘Shuangyou’ (SY) which belongs to V. amurensis, and cold-sensitive cultivar ‘Red Globe’ (RG) which belongs to Vitis vinifera L. were used to identify cold resistance genes. Cold-resistance quantitative trait locus (QTL) mapping was performed based on genetic population construction through interspecific crossing of ‘Shuangyou’ and ‘Red Globe’. Additionally, transcriptome analysis was conducted for the dormant buds of these two cultivars at different periods. Based on transcriptome analysis and QTL mapping, many new structural genes and transcription factors which relate to V. amurensis cold resistance were discovered, including CORs (VaCOR413IM), GSTs (VaGST-APIC, VaGST-PARB, VaGSTF9 and VaGSTF13), ARFs (VaIAA27 and VaSAUR71), ERFs (VaAIL1), MYBs (VaMYBR2, VaMYBLL and VaMYB3R-1) and bHLHs (VaICE1 and VabHLH30). Conclusions This discovery of candidate cold-resistance genes will provide an important theoretical reference for grape cold-resistance mechanisms, research, and cold-resistant grape cultivar breeding in the future.

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DgMYB2 improves cold resistance in chrysanthemum by directly targeting DgGPX1

Cited by 27 publications

References 29 publications

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

DgbZIP3 interacts with DgbZIP2 to increase the expression ofDgPODfor cold stress tolerance in chrysanthemum

Discovery of cold-resistance genes in Vitis amurensis using bud-based quantitative trait locus mapping and RNA-seq

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