DHEA effects on myocardial Akt signaling modulation and oxidative stress changes in aged rats

Jacob, Maria; Janner, Daiane da Rocha; Jahn, Matheus Parmegiani; Kucharski, Luiz Carlos; Belló‐Klein, Adriane; Ribeiro, Maria Flávia Marques

doi:10.1016/j.steroids.2009.08.005

Cited by 17 publications

(21 citation statements)

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“…DHEA induced apoptosis by down-regulating the Akt signaling pathway in GT1-7 hypothalamic neurons, when deprived of trophic support [62]. Recently, we have reported that acute (50 mg/kg) and chronic (10 mg/kg) DHEA treatment to 3 and 18 m rats, respectively, resulted in an elevation of pAkt/Akt ratio in myocardial as compared to control with the same dose and pattern of administration [32,35].…”

Section: Discussionmentioning

confidence: 95%

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Dehydroepiandrosterone effects on Akt signaling modulation in central nervous system of young and aged healthy rats

Janner

Jacob

Jahn

et al. 2010

The Journal of Steroid Biochemistry and Molecular Biology

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Dehydroepiandrosterone (DHEA) is a steroid synthesized in adrenal cortex as well as in the nervous system. DHEA effects on central nervous system (CNS) have been associated with several brain functions such as marked neurotrophic and neuroprotective activity. DHEA plasma concentration decreases steadily with aging and studies have reported an inverse correlation between levels of DHEA and neurological diseases age-associated. Nonetheless, its mechanisms of action are not yet fully understood. Akt signaling pathway is one protein kinase which has been related to be DHEA modulated. The goal of this study was to investigate whether short-term (6 or 24h) or chronic (5 weeks) DHEA treatment modulates Akt in CNS of adult (3 months) and aged (18 and 24 months) healthy rats. Hypothalamus and hippocampus homogenates were prepared to quantify total-Akt and phosphorylated Akt at Ser(473) (pAkt). The results here presented have shown that acute (50mg/kg) and chronic (10mg/kg) DHEA injections modulate total and pAkt levels. This effect was dose and time-dependent as well as age and tissue-dependent. In addition, the age variable also intervenes on total and pAkt levels expression independently of DHEA treatment.

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Section: Discussionmentioning

confidence: 95%

“…Three, eighteen and twenty-four months-old rats were injected subcutaneously with DHEA (10 mg/kg) or vehicle (vegetal oil), once a week, for 5 weeks. DHEA chronic administration protocol was based on other studies [33][34][35]. Each age group experiment was performed using 3 months untreated rats as a control group for comparison.…”

Section: Chronic Treatmentmentioning

confidence: 99%

Dehydroepiandrosterone effects on Akt signaling modulation in central nervous system of young and aged healthy rats

Janner

Jacob

Jahn

et al. 2010

The Journal of Steroid Biochemistry and Molecular Biology

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“…These changes would be related to DHEA treatment once its effects over oxidative stress have been largely described in literature [16,24,32,37,39,40]. Mastrocola et al [37] demonstrated the dual effect of DHEA in the liver, depending on the dosage.…”

Section: Discussionmentioning

confidence: 99%

“…Control groups received vehicle (vegetal oil). DHEA protocol administration was based on other studies [16,21,23,24]. Animals were housed in plastic cages (four animals in each) and received water and pelleted food ad libitum.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

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Dehydroepiandrosterone improves hepatic antioxidant reserve and stimulates Akt signaling in young and old rats

Jacob

Janner

Araújo

et al. 2011

The Journal of Steroid Biochemistry and Molecular Biology

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Dehydroepiandrosterone rehabilitate BRL‐3A cells oxidative stress damage induced by hydrogen peroxide

Zhao

et al. 2018

Journal Cellular Physiology

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This study aimed to investigate the molecular mechanism of dehydroepiandrosterone (DHEA) rehabilitated BRL-3A cells oxidative stress damage induced by hydrogen peroxide (H O ). Results showed that DHEA reversed the decrease of cell viability and ameliorated nuclear chromatin damage in H O -induced BRL-3A cells. DHEA increased the activities of superoxide dismutase, catalase, peroxidase, and glutathione peroxidase, and decreased the reactive oxygen species (ROS) production in H O -induced BRL-3A cells. DHEA attenuated the protein damage and lipid peroxidation, and reduced the apoptosis in H O -induced BRL-3A cells. The mRNA levels of Bax, Caspase-9, and Caspase-3 were decreased, while the Bcl-2 mRNA level was increased in H O -induced BRL-3A cells treated with DHEA. Our results showed that DHEA treatment increased the PI3K and p-Akt protein levels, while decreased the Bax and capase-3 protein levels in H O -induced BRL-3A cells. However, the rise in PI3K and p-Akt protein levels, and the decrease in Bax and capase-3 protein levels induced by DHEA treatment were reversed when the cells pretreated with LY294002 (PI3K inhibitor). These results indicated that DHEA ameliorated H O -induced oxidative damage by increasing anti-oxidative enzyme activities and ameliorating the protein damage and lipid peroxidation in BRL-3A cells. In addition, DHEA decreased the apoptosis by inhibiting caspase-3 and Bax protein levels and this action mainly achieved via the activation of PI3K/Akt signaling pathways in H O -induced BRL-3A cells. These results provided substantial information for DHEA as a nutritional supplement to treat oxidative stress and it related diseases in animals and humans.

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DHEA effects on myocardial Akt signaling modulation and oxidative stress changes in aged rats

Cited by 17 publications

References 50 publications

Dehydroepiandrosterone effects on Akt signaling modulation in central nervous system of young and aged healthy rats

Dehydroepiandrosterone effects on Akt signaling modulation in central nervous system of young and aged healthy rats

Dehydroepiandrosterone improves hepatic antioxidant reserve and stimulates Akt signaling in young and old rats

Dehydroepiandrosterone rehabilitate BRL‐3A cells oxidative stress damage induced by hydrogen peroxide

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