Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Kumari, Arpna; Kaur, Ravdeep

doi:10.32615/bp.2019.095

Cited by 9 publications

(4 citation statements)

References 53 publications

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“…The activity of GR was also observed to increase under the exposure of DEP in S. polyrhiza ( Cheng & Cheng, 2012 ). The activity of GR was observed to be altered significantly under the exposure of phthalates ( Kumari & Kaur, 2020a ). The other pollutants are also reported to be aggravated the GR activity in different crop plants ( Katerova et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Uptake of a plasticizer (di-n-butyl phthalate) impacts the biochemical and physiological responses of barley

Kumari

Kaur

2022

PeerJ

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Background DBP is one of the most commonly used plasticizers for imparting desirable properties to polymers. The introduction of phthalates is reported to have occurred in the late 1920s, and there has been a significant rise in their release into the environment in past decades due to a lack of covalent bonding with the parent matrix. Because of their numerous applications in day-to-day life, phthalates have become ubiquitous and also classified as endocrine disruptors. Hence, several studies have been conducted to investigate the phthalate-mediated toxicities in animals; however, plants have not been explored to the same amount. Methods Therefore, in the present study, the accumulation and translocation along with morpho-physiological perturbations in barley plants after 15, 30, 60, and 120 days of exposure to di-n-butyl phthalate (DBP) are investigated using standard protocols. Results The maximal accumulation and translocation of DBP in the roots and shoots of barley plants was observed after 60 days of exposure. The exposure of DBP from 15 to 120 days was recorded to decline all the morphological indices (i.e., dry weight, net primary productivity, seed number per spike, and seed weight) of barley plants. The pigments content declined under DBP treatment for all exposure durations except 120 days exposure. Carbohydrate content increased after 15–30 days of exposure afterward it was observed to be decreased under 60 and 120 days of exposure. The protein content was declined in DBP stressed plants for 15–120 days. Proline content was increased in all exposure durations and maximal percent increase was recorded in 120 days of exposure. MDA content showed an increase at earlier exposure durations then followed by a decline in long-term exposure. Hydrogen peroxide content increased at all exposure durations. There were significant alterations observed in the activities of all antioxidative enzymes in comparison to the control. Furthermore, DBP stressed plants after 60 days were analyzed for the macromolecular variations using Fourier transform infrared spectroscopy (FTIR). Conclusion Thus, the outcomes of the current work provide an appraisal of phthalates’ uptake and translocation mediated phytotoxic responses in barley plants. These observations can help in developing genetically modified edible plants that are resistant to phthalates uptake, thereby ensuring food security.

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Section: Discussionmentioning

confidence: 99%

Uptake of a plasticizer (di-n-butyl phthalate) impacts the biochemical and physiological responses of barley

Kumari

Kaur

2022

PeerJ

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“…: 2147-4249 environment, and resistance to microbial degradation is the main factors for their ubiquitous presence in the air, water, and food (Liu et al, 2014;Kumari and Kaur, 2020). Humans get exposed to phthalates through different pathways like inhalation, ingestion, and direct contact through the skin which can cause developmental and reproductive toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…. Operating Conditions of HPLC for phthalate estimation(Kumari et al, 2020).PhthalatesDibutyl phthalate (DBP), Benzyl butyl phthalate (BBP) and Dimethyl phthalate (DMP), Diethyl phthalate (DEP), Diallyl phthalate (DAP) Column and column temperature C18 column and 40°C Flow rate and injection volume 0.9 ml/min and 20 µL Detection wavelength…”

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confidence: 99%

Seasonal fluctuations in phthalates’ contamination in pond water: A case study

Rajput

Kumari

Sharma

et al. 2023

EJSS

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Phthalates are endocrine disruptors, reported to cause deformities and reproductive damages in animals. Numerous studies reported the presence of phthalates in water samples of rivers, wetlands, and estuaries, while the scenario in case of ponds is different, however they are reported as an integral part of biosphere. In this study, the level of phthalates’ contamination in the water samples collected from the different ponds of Amritsar district for four consecutive seasons in two years was analysed. The maximal level of phthalate contamination was found in samples collected during the monsoon season (July 2015) of first year of sampling followed by post-monsoon (October 2015) and winter season (January 2016). S8 sampling site was found to be the most phthalate contaminated site followed by S1=S11>S2=S9=S4=S5=S7>S6=S3>S10. Benzyl butyl phthalate was most abundant (found in 32% water samples) followed by di-n-butyl and dimethyl phthalate, while diallyl phthalate and diethyl phthalate were not detected. The two main drivers for these seasonal variations were observed to be temperature and precipitation. Hence, this data will be useful to explain the temporal and spatial distributions of phthalates in aquatic ecosystem, as well as to devise cost-effective ways to reduce their ecological footprints.

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“…Other than physical properties like inhibition of root or shoot elongation, and reduction of biomass, or disruption of photosynthetic organ, PAE also induced activity of antioxidant enzymes. In barley plants (Horderum vulgare), DBP contamination cause reduction in superoxide dismutase, ascorbate peroxidase, glutathione reductase activity, while on the other hand increase guaiacol peroxidase and catalase activity (Kumari & Kaur, 2020).…”

Section: 12mentioning

confidence: 99%

Phthalate ester biodegradation in agricultural soil using exogenous bacterial consortium under fertilizer addition

Putri

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Phthalate esters (PAEs) have been classified as a priority contaminant due to their endocrine-disrupting properties and abundance in the environment. Previously, several PAE-degrading consortia were enriched from landfill soil. This study aimed to initially select a consortium with efficient degradation activity, to characterize based on degradation kinetics and community dynamics, and to evaluate bioaugmentation feasibility while maintaining activity through continuous transfer. It was obtained based on enriched consortium selection that LF-NK-DEHP could simultaneously degrade 92.9% 100 mg l-1 DBP and 63.4% 100 mg l-1 DEHP within seven days. Kinetic degradation revealed DBP degradation by LF-NK-DEHP to fit the modified Gompertz model (R2 = 0.92,� t1/2 =1.49 days, 500 mg l-1 DBP). On the other hand, DEHP degradation kinetics fit a zero-order kinetic model (R2 = 0.94, t1/2 =7.23 days, 500 mg l-1 DEHP). Community dynamics study revealed a significant correlation between the genus Microbacterium to DBP degradation and the genus Rhodococcus to DEHP degradation. Genus Pigmentiphaga was reported to correlate with both PAEs. In addition, continuous transfer of LF-NK-DEHP could maintain PAE degradation activity. Bioaugmentation of enriched consortium LF-NK-DEHP revealed insignificant degradation rate compared to natural attenuation. However, fertilizer addition was observed to improve the bioaugmentation performance. Bioaugmentation added with fertilizer could degrade 200 mg kg-1 DEHP up to 75.87% within 25 days while removing 200 mg kg-1 DBP altogether within three days. This was further confirmed through alleviation of PAE intermediates phytotoxicity. Upon completion of this research, new information was acquired, including methods to maintain enriched consortia degradation activity, potential novel PAE-degraders, and feasibility of bioaugmentation with exogenous enriched consortium for PAE bioremediation.

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Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense response

Cited by 9 publications

References 53 publications

Uptake of a plasticizer (di-n-butyl phthalate) impacts the biochemical and physiological responses of barley

Uptake of a plasticizer (di-n-butyl phthalate) impacts the biochemical and physiological responses of barley

Seasonal fluctuations in phthalates’ contamination in pond water: A case study

Phthalate ester biodegradation in agricultural soil using exogenous bacterial consortium under fertilizer addition

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