Hypoxia plays a major role in limiting the construction of the high-altitude mine. Understanding the effect and the distribution of diffused oxygen supply at different altitudes is the premise for the design of a mine with oxygen supply system and the full utilization of oxygen resources. For the optimal design of an oxygen supply mode and ventilation system, a multi-component fluid model of the diffused oxygen supply of a single-head tunnel was developed. This study reveals the variation law of the average oxygen mass fraction at different altitudes. The relationship between the distance from the heading face and the oxygen mass fraction at each altitude was fitted. The results show that the distribution of oxygen mass fraction in a single head tunnel at different altitudes presents a similar trend of increasing first and then decline. In addition, the change of the average oxygen mass fraction with the increase of altitude is not linear, and the dispersion loss is larger in high-altitude areas. The largest oxygen enrichment area is distributed at the altitudes of 4000–4500 m. This study provides theoretical support for improving the hypoxic environment of high-altitude metal mines excavation work.