Diabetes and the kidney

Chowdhury, Tahseen A; Ali, Omer

doi:10.7861/clinmed.2021-0144

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Renal injury is a serious microvascular complication of diabetes mellitus, a highly prevalent disease currently reaching epidemic proportions [1]. Diabetic nephropathy (DN) occurs in up to 40% of diabetic patients, contributing to a worse quality of life and representing a substantial economic burden [2,3].…”

Section: Introductionmentioning

confidence: 99%

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

Martínez-Arroyo

Flores-Chova

Sanchez-Garcia

et al. 2023

Biology

View full text Add to dashboard Cite

Diabetic nephropathy is a major complication in diabetic patients. Podocytes undergo loss and detachment from the basal membrane. Intra- and intercellular communication through exosomes are key processes for maintaining function, and the Rab3A/Rab27A system is an important counterpart. Previously, we observed significant changes in the Rab3A/Rab27A system in podocytes under glucose overload, demonstrating its important role in podocyte injury. We investigated the implication of silencing the Rab3A/Rab27A system in high glucose-treated podocytes and analysed the effect on differentiation, apoptosis, cytoskeletal organisation, vesicle distribution, and microRNA expression in cells and exosomes. For this, we subjected podocytes to high glucose and transfection through siRNAs, and we isolated extracellular vesicles and performed western blotting, transmission electron microscopy, RT-qPCR, immunofluorescence and flow cytometry assays. We found that silencing RAB3A and RAB27A generally leads to a decrease in podocyte differentiation and cytoskeleton organization and an increase in apoptosis. Moreover, CD63-positive vesicles experienced a pattern distribution change. Under high glucose, Rab3A/Rab27A silencing ameliorates some of these detrimental processes, suggesting a differential influence depending on the presence or absence of cellular stress. We also observed substantial expression changes in miRNAs that were relevant in diabetic nephropathy upon silencing and glucose treatment. Our findings highlight the Rab3A/Rab27A system as a key participant in podocyte injury and vesicular traffic regulation in diabetic nephropathy.

show abstract

Section: Introductionmentioning

confidence: 99%

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

Martínez-Arroyo

Flores-Chova

Sanchez-Garcia

et al. 2023

Biology

View full text Add to dashboard Cite

show abstract

“…Diabetic nephropathy (DN), also known as diabetic kidney disease (DKD) [ 1 , 2 , 3 ], is a severe complication of diabetes mellitus [ 4 , 5 ]. Approximately 30% of diabetic patients can develop DN [ 6 , 7 , 8 ], which is also a chronic kidney disease and can progress to end-stage renal failure [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

The Nicotinamide/Streptozotocin Rodent Model of Type 2 Diabetes: Renal Pathophysiology and Redox Imbalance Features

2022

Biomolecules

View full text Add to dashboard Cite

Diabetic nephropathy (DN) is a common complication of diabetes mellitus. While there has been a great advance in our understanding of the pathogenesis of DN, no effective managements of this chronic kidney disease are currently available. Therefore, continuing to elucidate the underlying biochemical and molecular mechanisms of DN remains a constant need. In this regard, animal models of diabetes are indispensable tools. This review article highlights a widely used rodent model of non-obese type 2 diabetes induced by nicotinamide (NA) and streptozotocin (STZ). The mechanism underlying diabetes induction by combining the two chemicals involves blunting the toxic effect of STZ by NA so that only a percentage of β cells are destroyed and the remaining viable β cells can still respond to glucose stimulation. This NA-STZ animal model, as a platform for the testing of numerous antidiabetic and renoprotective materials, is also discussed. In comparison with other type 2 diabetic animal models, such as high-fat-diet/STZ models and genetically engineered rodent models, the NA-STZ model is non-obese and is less time-consuming and less expensive to create. Given that this unique model mimics certain pathological features of human DN, this model should continue to find its applications in the field of diabetes research.

show abstract

Diabetes and the kidney

Cited by 2 publications

References 19 publications

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

The Nicotinamide/Streptozotocin Rodent Model of Type 2 Diabetes: Renal Pathophysiology and Redox Imbalance Features

Contact Info

Product

Resources

About