Diabetex: A novel approach for diabetic wound healing

El-Bahy, Alshaymaa Amin Zaki; Aboulmagd, Yara Mohamed; Zaki, Mohanad

doi:10.1016/j.lfs.2018.06.020

Cited by 19 publications

(25 citation statements)

References 23 publications

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“…It is postulated that a diabetes-like condition occurs and, as a result, wound healing is delayed. The results of the present study are supported by a previous report that demonstrated improved epithelial healing following the administration of metformin in a diabetic rat model presenting cleft wounds on the epithelium [33]. Moreover, there are various risk factors for the onset of MRONJ.…”

Section: Discussionsupporting

confidence: 90%

Effects of metformin on the prevention of bisphosphonate-related osteonecrosis of the jaw-like lesions in rats

et al. 2021

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In this study, we aimed to investigate the effect of glucose metabolism on bone healing after tooth extraction in an osteoporosis rat model administered zoledronic acid (ZA) and dexamethasone (DX). Methods: In total, 24 male Wistar rats (4 weeks old) were randomly assigned to four groups: Control (subcutaneous physiological saline), ZD (subcutaneous ZA and DX twice a week), Ins+ZD (subcutaneous insulin followed by ZD treatment), and Met+ZD (oral metformin followed by ZD treatment). Blood was collected every two weeks. Two weeks after treatment initiation, the first molar tooth on the right maxilla was extracted from all rats. Four weeks later, the rats were sacrificed, and bone healing was assessed. Maxillae samples were fixed and scanned using micro-computed tomography for quantifying areas of bone defects. Hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate bone apoptosis and osteoclast number. Results: In all experimental groups, body weight was statistically lower than that in the Control group, with no changes observed in uncarboxylated osteocalcin concentrations. The radiological analysis revealed that insulin or metformin administration improved healing in the tooth extraction socket (p < 0.01). Histological examination revealed that the osteonecrosis area was reduced in the Ins+ZD and Met+ZD groups (p < 0.01). TRAP staining presented increased osteoclast numbers in the ZD group when compared with that observed in the Control. Conclusions: Tooth extraction with long-term ZA and DX administration inhibited bone remodeling and induced bisphosphonate-related osteonecrosis of the jaw-like lesions. Metformin exerted protective effects against osteonecrosis of the jaw.

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Section: Discussionsupporting

confidence: 90%

Effects of metformin on the prevention of bisphosphonate-related osteonecrosis of the jaw-like lesions in rats

et al. 2021

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“…Diabetic patients present impairment in healing due to imbalance of the inflammatory response, with prolonged accumulation of inflammatory cells and excessive production of inflammatory cytokines, alteration of collagen synthesis and reduction of tensile strength 6,7,14,19,22 . These factors when associated result in reduced wound resistance and therefore increased dehiscence which, added to the larger risk of infections in this group, result in increased hospitalization time and mortality rate 3,6,7,14,22 .…”

Section: Introductionmentioning

confidence: 99%

“…Diabetic foot ulcer is the leading cause of hospitalization of DM patients in developed countries and is among the leading causes of morbidity and mortality, with an average healing time of six months and need for amputation in up to one third of the cases. This complication still demands studies addressing the complexity of its management 3,6,7,14 .…”

Section: Introductionmentioning

confidence: 99%

Effects of Probiotics Supplementation on Skin Wound Healing in Diabetic Rats

Campos

Tagliari

Casagrande

et al. 2020

ABCD, arq. bras. cir. dig.

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Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

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“…Wound healing is a complex process that requires an inflammatory reaction, wound clotting, re-epithelialization, remodeling regulation, and control of stem cells [4,5]. e epidermal stem cell (EpSC), as a specific stem cell of skin tissue, is increasingly recognized as a crucial component in diabetic wound healing.…”

Section: Introductionmentioning

confidence: 99%

The Potential Role of Cycloastragenol in Promoting Diabetic Wound Repair In Vitro

Cao

Yang

et al. 2019

BioMed Research International

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Background. Refractory wound healing is a severe complication of diabetes with a significant socioeconomic burden. Whereas current therapies are insufficient to accelerate repair, stem cell-based therapy is increasingly recognized as an alternative that improves healing outcomes. The aim of the present study is to explore the role of cycloastragenol (CAG), a naturally occurring compound in Astragali Radix, in ameliorating refractory cutaneous wound healing in vitro, which may provide a new insight into therapeutic strategy for diabetic wounds. Methods. Human epidermal stem cells (EpSCs) obtained from nine patients were exposed to CAG, with or without DKK1 (a Wnt signaling inhibitor). A lentiviral short hairpin RNA (shRNA) system was used to establish the telomerase reverse transcriptase (TERT) and β-catenin knockdown cell line. Cell counting kit-8, scratch wound healing, and transwell migration assay were used to determine the effects of CAG in cell growth and migration. The activation of TERT, β-catenin, and c-Myc was determined using real-time qPCR and western blot analysis. Chromatin immunoprecipitation (ChIP) was performed to evaluate the associations among CAG, TERT, and Wnt/β-catenin signals. Results. CAG not only promoted the proliferation and migration ability of EpSCs but also increased the expression levels of TERT, β-catenin, c-Myc. These effects of CAG were most pronounced at a dose of 0.3 μM. Notably, the CAG-promoted proliferative and migratory abilities of EpSCs were abrogated in TERT and β-catenin-silenced cells. In addition, the ChIP results strongly suggested that CAG-modulated TERT was closely associated with the activation of Wnt/β-catenin signaling. Conclusion. Our data indicate that CAG is a TERT activator of EpSCs and is associated with their proliferation and migration, a role it may play through the activation of Wnt/β-catenin signaling.

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Diabetex: A novel approach for diabetic wound healing

Cited by 19 publications

References 23 publications

Effects of metformin on the prevention of bisphosphonate-related osteonecrosis of the jaw-like lesions in rats

Effects of metformin on the prevention of bisphosphonate-related osteonecrosis of the jaw-like lesions in rats

Effects of Probiotics Supplementation on Skin Wound Healing in Diabetic Rats

The Potential Role of Cycloastragenol in Promoting Diabetic Wound Repair In Vitro

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